LOCUS       Exported     3410 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3410
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3376
                     /label="Mfl084"
                     /note="Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity."
                     /translation="MSRKDMSENNLHSVEKMVLAIAMHSPNALPDIMTGLVADDFLDSTHKIIFQGIIELHSQSKEVTISSVANQIEKKGNLESIGGIQRLQDIALDFFSDEGIENFIEDIFYASSSRKFDAALRRVQNLRREEQMDIESSINEIQKELLKIDLNAQKNDVQSIHASTESLIKKIQELEKRSETLTGIPTDLFELDEITSGLQEGDLIILAARPSMGKTAFALNLAYNAARHKNGVAVFSLEMPAEQLTQRIMTMVSKIDSKKLRTGKNISKAEWSNLLASKEIVSQLPIYIDDTPGITVQQIQSKLYKLKRDHDVKFCVVDYLQLISSTNGSADRQNEISNISRQLKRVARELRIPIVCLSQLSRSVEKREDKRPIMSDLRDSGAIEQDADIIMFLYRDDYYKHKDGNEEENKIVDQVSATDLILSKHRNGATGTINILFDKSHGKFMDKG"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATGCAAAG CTATTAGCGC GGGTCTCAAA TGAGCCGTAA
     2041 GGACATGAGC GAAAATAATC TGCATTCAGT TGAGAAAATG GTCCTGGCGA TCGCAATGCA
     2101 CAGCCCGAAT GCACTGCCGG ACATCATGAC GGGCCTGGTC GCAGACGACT TCCTGGACAG
     2161 CACTCACAAG ATCATCTTTC AGGGCATTAT TGAGTTGCAC AGCCAAAGCA AAGAAGTCAC
     2221 GATCAGCAGC GTCGCGAACC AAATTGAAAA GAAAGGTAAT CTGGAGAGCA TTGGTGGTAT
     2281 CCAACGTTTG CAAGATATTG CACTCGACTT CTTTAGCGAC GAAGGCATTG AGAACTTCAT
     2341 TGAGGACATC TTCTACGCGA GCAGCAGCCG TAAGTTCGAC GCAGCTCTGC GCCGTGTGCA
     2401 GAACTTAAGA CGTGAGGAGC AAATGGATAT TGAAAGCAGC ATTAACGAGA TTCAGAAAGA
     2461 GCTGCTGAAG ATCGATCTGA ACGCGCAAAA GAACGACGTA CAGTCCATCC ACGCTAGCAC
     2521 GGAGAGCCTG ATTAAAAAGA TTCAAGAGCT GGAGAAACGC TCCGAAACCC TGACCGGCAT
     2581 CCCGACTGAC CTGTTTGAGC TGGACGAAAT CACCTCGGGT CTGCAGGAAG GTGATCTGAT
     2641 TATTCTGGCG GCCCGTCCTT CTATGGGTAA AACGGCATTT GCCCTGAACC TGGCTTATAA
     2701 TGCTGCCCGC CACAAGAATG GTGTCGCGGT GTTCAGCCTG GAGATGCCGG CAGAGCAACT
     2761 GACGCAACGT ATTATGACCA TGGTTTCTAA GATCGACAGC AAAAAGCTGC GTACGGGCAA
     2821 AAATATTAGC AAAGCGGAAT GGTCTAACTT GCTGGCAAGC AAAGAAATTG TGAGCCAATT
     2881 GCCGATTTAC ATCGATGATA CGCCGGGCAT CACTGTGCAA CAGATCCAGA GCAAATTGTA
     2941 TAAGCTGAAA CGCGATCACG ACGTTAAGTT CTGCGTTGTT GATTATCTGC AACTGATCAG
     3001 CTCTACCAAC GGTAGCGCAG ATCGCCAAAA TGAGATCAGC AATATTAGCC GCCAACTGAA
     3061 ACGTGTTGCC CGCGAGCTGC GTATCCCGAT CGTGTGCCTG AGCCAGCTGA GCCGTAGCGT
     3121 GGAGAAGCGT GAGGACAAAC GCCCGATTAT GTCCGATCTG CGCGACAGCG GTGCGATTGA
     3181 GCAGGATGCC GATATCATCA TGTTTCTGTA TCGCGACGAC TACTATAAAC ACAAAGACGG
     3241 TAACGAAGAG GAGAATAAGA TCGTTGACCA GGTGAGCGCG ACCGATCTGA TTCTGAGCAA
     3301 GCATCGTAAC GGTGCAACCG GCACGATTAA TATCCTGTTC GATAAGAGCC ATGGTAAATT
     3361 CATGGATAAA GGCTGAAGAG CTTAGAGACC CCCCGTGAAT GCTATGTTCT
//