LOCUS Exported 3496 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3496 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1534..1581 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3429..3445 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3446..3477 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1462 /note="" /translation="MKKLLSIITALTVAASATSSLVSCKVITNGFDIEITEYKAAGNLKYTFDTNAGEYVPKTDYTTTEADVKKALDGATGTFASSLVTDMINSLFFNANLSVTQNSKDIWQYLFRTDKEGIYADGQYRTAGLNSSQNEEIDKYYSEIRNITSLLGLMQYSEWTNSNSKEVQSLDESGKPFVLSKETLDFINNETNMFESSNNQGITNVKINSIPKLYEGNIGLATKSDNESSDAEETVYEPINITSYEKKYISKETNYNAKTAKETEDSKGEKGYVDYISGTNLSANFFSQLLISSPQADSKPELDIQVQRVEKQSDTSKENFKKQGLVLNSKKDTDPIVFEYTEDEKGNRTSYAYGYSLIPLAPINLEMTYNNDRDLKTGEPKPGSQNYKINLTIDGLSAAFKPVLSFTSNLDLEGNKTDKDGTPYVGWRFMNYQFNSKNIIGYDEQGNQFTDKPSKKMTATNKKFNDFNITDLTFEKA" /label="Mfl085" primer_bind complement(1586..1602) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1669..2257) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2448..3308) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3309..3413) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAACACGTGG CAAACATTCC GGTCTCAAAT GAAAAAGTTG CTCAGCATTA TCACGGCGCT 61 GACGGTTGCT GCTAGCGCGA CGTCCAGCCT GGTTTCATGT AAAGTTATTA CCAACGGCTT 121 TGATATCGAA ATTACGGAGT ACAAAGCAGC GGGTAATCTG AAATACACCT TTGACACGAA 181 CGCTGGTGAG TACGTTCCTA AGACCGACTA CACCACCACC GAGGCGGACG TTAAAAAAGC 241 CCTTGATGGC GCAACGGGTA CGTTCGCAAG CTCCCTGGTC ACGGATATGA TCAATTCGCT 301 GTTTTTCAAT GCGAATCTGT CTGTTACCCA GAACAGCAAG GATATCTGGC AATACCTGTT 361 CCGCACCGAT AAGGAAGGTA TTTATGCTGA CGGCCAGTAC CGTACGGCGG GTTTGAACAG 421 CTCCCAGAAT GAGGAAATTG ATAAGTACTA TTCCGAGATC CGTAACATTA CCTCGCTGCT 481 GGGTCTGATG CAATATAGCG AATGGACCAA TAGCAACAGC AAGGAAGTTC AATCCTTAGA 541 TGAATCTGGT AAGCCGTTTG TTCTGAGCAA AGAAACCCTG GACTTTATCA ATAACGAAAC 601 CAATATGTTC GAATCTAGCA ATAACCAGGG TATTACGAAT GTTAAGATTA ACAGCATCCC 661 TAAACTGTAC GAGGGTAATA TTGGTCTGGC AACGAAAAGC GATAACGAGA GCTCGGATGC 721 GGAGGAAACG GTCTATGAGC CAATCAATAT TACCAGCTAC GAAAAGAAAT ACATCTCGAA 781 GGAAACGAAC TACAACGCCA AGACGGCAAA GGAAACGGAG GACTCCAAAG GTGAAAAAGG 841 TTACGTTGAC TACATCAGCG GTACGAACCT GAGCGCGAAC TTCTTCAGCC AACTGCTGAT 901 CTCAAGCCCT CAGGCGGATT CTAAACCGGA GCTGGATATT CAGGTACAGC GTGTCGAAAA 961 ACAGTCCGAC ACCTCGAAAG AAAACTTCAA GAAACAGGGT CTGGTTCTGA ACAGCAAGAA 1021 GGACACTGAC CCGATCGTGT TTGAATATAC GGAGGATGAA AAGGGTAACC GTACCTCCTA 1081 TGCGTATGGC TATTCCTTGA TCCCGCTGGC ACCGATCAAT CTGGAAATGA CCTATAACAA 1141 TGATCGCGAT CTGAAAACTG GCGAGCCGAA GCCGGGTAGC CAAAACTATA AAATTAATCT 1201 GACGATCGAT GGCCTGAGCG CTGCTTTTAA GCCGGTTCTG AGCTTCACTA GCAACCTGGA 1261 TCTGGAGGGT AATAAGACCG ATAAAGACGG TACGCCTTAT GTTGGTTGGC GCTTTATGAA 1321 CTATCAGTTT AATTCTAAAA ATATTATTGG TTATGACGAG CAGGGTAACC AATTCACCGA 1381 CAAGCCGAGC AAAAAGATGA CTGCGACTAA CAAGAAATTC AATGACTTTA ACATTACGGA 1441 CCTGACCTTC GAAAAGGCCT GAAGAGCTTA GAGACCCAGT CCTTGCCAAT CGAAGTCGCT 1501 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA 1561 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG 1621 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT 1681 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 1741 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 1801 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 1861 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 1921 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 1981 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 2041 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 2101 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 2161 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 2221 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC 2281 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 2341 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 2401 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 2461 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 2521 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 2581 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 2641 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 2701 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 2761 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 2821 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 2881 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 2941 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 3001 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 3061 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 3121 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 3181 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 3241 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 3301 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 3361 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC 3421 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG 3481 TCAGGTGGAG TGGGAG //