LOCUS       Exported     3818 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3818
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3784
                     /label="Mfl092"
                     /note=""
                     /translation="MSKIKFMALGGQDERGKNLFVLDIDDNLFILDAGVKYPDKGILGVDIVTPKLDYLKNNKQKIKGIFLTNSASYNMGSVPYILKTMDVPVYCNEITQLVGKIKISRMRIKNTKDQNFVVVKDKQILDFGKVKVEVFRTTSASPQSYGYAFHTEEGTIVYAGDYIIDGKEQSYFSTDFNHISEIGKKGVLALIADSEYASRSGFTVPNHKIENFISTSFKEKKTKIAIGIFEEDIFKLGEICMAAKENNRKIAVYGRTMTEILKSNLINENLQIVPEDIITVDEYMKSENGLLIISGTGDVLYSKLAKIATGNDEVVEFTEKDLIILATPPAPGVEKRHAQILDELARTDARLLALSDRNIWSMHASYEDIKVFTSILNPKYFIPVKSLFKDSLKAEKAAIEAGVNERNIVILDNGQVANISKNSISISDKKIDIGNSYVDQAGVGDVGAIVLNERKLLATDGVMIIGATIDSRNKELISMIDTQMRGVLYIKEENPIFKIIQKEIESLLEQGQSQFKENPSKYDVNEVKKDIATRVRTLIKQESGKQPIVLVIVNEYDGKDYVFKPRNNSNRNYKNNNNNKKGSN"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATGTCCTT AGGCAGTCGT CGGTCTCAAA TGTCTAAGAT
     2041 TAAATTCATG GCGCTGGGTG GCCAGGACGA GCGCGGCAAG AATCTGTTTG TCCTGGACAT
     2101 TGACGACAAT CTGTTCATCC TGGATGCGGG TGTCAAATAC CCTGATAAGG GTATTCTGGG
     2161 TGTCGATATC GTTACCCCGA AGCTGGATTA CCTGAAGAAC AACAAGCAAA AGATTAAAGG
     2221 TATTTTCTTG ACGAATAGCG CAAGCTATAA CATGGGCTCG GTACCGTACA TCCTCAAGAC
     2281 CATGGACGTT CCGGTGTATT GCAACGAAAT CACTCAACTG GTGGGTAAAA TTAAAATCAG
     2341 CCGTATGCGT ATTAAGAATA CCAAGGATCA AAACTTCGTT GTCGTTAAAG ACAAGCAAAT
     2401 CCTGGACTTT GGCAAGGTCA AAGTTGAAGT TTTTCGCACG ACGTCCGCCA GCCCGCAAAG
     2461 CTATGGTTAC GCGTTCCATA CCGAAGAAGG CACCATCGTT TATGCCGGCG ATTATATCAT
     2521 CGACGGTAAG GAACAAAGCT ATTTTTCAAC CGACTTCAAT CACATCAGCG AAATCGGTAA
     2581 AAAAGGCGTG CTGGCACTCA TTGCCGACTC GGAGTACGCT TCACGTAGCG GTTTTACCGT
     2641 CCCTAACCAT AAAATTGAGA ACTTCATCTC GACGAGCTTT AAAGAGAAGA AAACCAAAAT
     2701 TGCAATTGGC ATTTTCGAAG AGGACATCTT CAAGTTGGGT GAGATCTGCA TGGCCGCGAA
     2761 AGAGAACAAT CGTAAAATTG CAGTGTACGG TCGTACCATG ACCGAGATCC TGAAAAGCAA
     2821 TCTGATCAAC GAGAATCTGC AGATTGTTCC GGAGGACATT ATTACCGTTG ACGAGTACAT
     2881 GAAAAGCGAA AACGGTCTGC TGATCATCTC CGGTACCGGT GATGTGCTGT ATTCCAAACT
     2941 GGCCAAGATC GCAACCGGTA ACGATGAGGT TGTCGAATTC ACCGAGAAGG ACCTGATTAT
     3001 TCTGGCGACT CCACCGGCAC CGGGTGTCGA AAAGCGTCAC GCACAGATCC TGGACGAGCT
     3061 GGCGCGTACC GACGCGCGTC TGCTGGCCCT GAGCGACCGT AACATTTGGA GCATGCACGC
     3121 GAGCTACGAG GATATCAAGG TTTTCACCTC TATTTTGAAC CCGAAGTATT TCATCCCGGT
     3181 AAAGAGCCTT TTCAAGGACA GCTTAAAAGC AGAGAAGGCG GCTATCGAGG CGGGTGTTAA
     3241 TGAGCGTAAC ATCGTGATTC TGGACAACGG TCAAGTTGCG AACATTTCAA AGAATTCCAT
     3301 CAGCATCTCG GACAAAAAGA TTGACATCGG CAACAGCTAC GTCGATCAGG CGGGTGTCGG
     3361 TGACGTGGGT GCAATTGTTC TGAATGAACG TAAACTGCTG GCGACTGACG GTGTGATGAT
     3421 TATCGGCGCG ACCATCGATA GCCGTAACAA GGAACTGATT AGCATGATTG ACACCCAAAT
     3481 GCGCGGCGTG TTGTATATCA AAGAAGAAAA CCCGATTTTC AAAATCATTC AAAAAGAAAT
     3541 TGAAAGCCTG CTGGAGCAAG GTCAAAGCCA GTTCAAGGAA AACCCGAGCA AATATGACGT
     3601 TAACGAAGTT AAAAAAGATA TCGCTACCCG TGTTCGCACG CTTATCAAAC AAGAATCCGG
     3661 TAAGCAGCCG ATCGTTCTGG TTATTGTGAA CGAATACGAC GGTAAAGATT ATGTTTTCAA
     3721 GCCACGCAAC AACAGCAATC GCAACTATAA AAACAATAAC AATAACAAGA AAGGTAGCAA
     3781 CTGAAGAGCT TAGAGACCGT TGCCCTTGCT GAGATACT
//