LOCUS Exported 3632 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3632 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3598 /label="Mfl096" /note="" /translation="MATNKDKIISLQDVVVKFNVRGRMLTAIRNVSFDIYDGETVAIVGESGSGKSVLTKTLTNMLESNGYISNGTIMYFPTEESKNNSETAVKEDVNLVNYHKGSLTSDTRRKIKKSNYKTINNAKIRLEALNARLGVKGVDTDDILAKIKQESEIIHDAYYELSTFSRLRKRTVLRLTPTMKEISSKKRNLSLIKGRQILAGLRILAEQEFLTEFELNLKHVLEKLKNGETVEEHEVNTLLAAWKFQTTPAWLNKREAIKKLKQVRGGTIATIFQDPMTSLNPLLSVGFQISEAIRLHNKVSRHAAKQQAIELMNKVGIPNAEKRYHDIPGKYSGGMRQRIVIAIALACKPKVLICDEPTTALDVTIQAQILELIKELKKEFNLTVIFITHDLGVVANVADRVAVLYAGQIIEYGTVKDIFFDARHPYTWALLSSLPQLGTKGEELFAITGTPPSLFNKIKGDAFAPRNKFALAIDFEYNPPMFEISKTHGARTWLLDPRAPKVEKPKMLENLKEAVSEAKVGE" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAGGAACTA TGGCATCGAG CGGTCTCAAA TGGCCACCAA 2041 CAAGGATAAG ATTATTAGCC TGCAAGACGT TGTGGTCAAG TTTAACGTGA GAGGCCGTAT 2101 GTTGACCGCG ATCCGTAATG TGAGCTTTGA CATTTATGAC GGTGAAACCG TCGCGATCGT 2161 GGGCGAATCC GGTAGCGGTA AAAGCGTGCT GACCAAAACC TTGACGAATA TGCTGGAAAG 2221 CAACGGCTAT ATTAGCAACG GTACCATTAT GTATTTCCCG ACCGAGGAGT CCAAAAACAA 2281 TAGCGAAACG GCTGTGAAAG AGGACGTTAA TTTGGTGAAC TACCACAAGG GTAGCCTGAC 2341 CAGCGATACC CGCCGTAAGA TCAAAAAGAG CAACTATAAG ACCATTAATA ACGCAAAGAT 2401 CCGCCTGGAA GCGCTGAACG CGCGTCTGGG TGTCAAGGGT GTGGACACGG ACGACATTCT 2461 GGCGAAGATC AAACAAGAAT CGGAGATCAT TCACGACGCA TACTACGAGC TGAGCACCTT 2521 CAGCCGTCTG CGTAAACGCA CCGTTTTACG TCTGACGCCG ACGATGAAGG AAATCAGCTC 2581 GAAGAAGCGT AACCTGTCTT TAATCAAAGG TCGTCAAATT CTGGCTGGTC TGCGTATCCT 2641 GGCTGAGCAA GAGTTCCTTA CGGAGTTCGA ACTGAACCTG AAACACGTGC TGGAAAAATT 2701 GAAAAACGGT GAAACCGTCG AGGAACACGA GGTGAACACG CTGCTGGCGG CCTGGAAGTT 2761 CCAGACGACG CCGGCCTGGC TCAATAAGCG TGAAGCAATT AAGAAACTGA AACAGGTACG 2821 TGGTGGTACC ATTGCAACCA TCTTCCAAGA CCCAATGACT AGCTTGAATC CGCTGCTGAG 2881 CGTCGGTTTC CAAATCTCCG AAGCAATCAG ATTGCATAAT AAGGTGAGCC GCCACGCGGC 2941 AAAACAGCAG GCGATCGAGC TGATGAACAA AGTGGGCATC CCGAACGCTG AGAAGCGCTA 3001 CCACGACATT CCGGGTAAGT ATAGCGGTGG TATGCGCCAG CGCATCGTTA TTGCGATTGC 3061 GCTGGCCTGT AAGCCGAAAG TCCTGATTTG TGATGAACCG ACCACGGCCC TCGACGTCAC 3121 CATCCAGGCG CAAATCCTGG AACTGATTAA AGAACTGAAA AAAGAATTTA ATCTGACCGT 3181 TATCTTTATC ACCCATGACC TGGGCGTGGT CGCTAACGTC GCGGACCGTG TGGCTGTCCT 3241 GTACGCGGGT CAGATTATCG AGTATGGTAC CGTCAAGGAC ATCTTTTTTG ATGCACGTCA 3301 TCCGTATACT TGGGCGCTGC TGAGCTCCCT CCCGCAACTG GGTACCAAAG GTGAGGAGCT 3361 GTTCGCAATC ACGGGCACGC CGCCGAGCCT GTTCAATAAA ATCAAGGGTG ATGCCTTTGC 3421 TCCACGTAAC AAGTTTGCTC TGGCAATTGA TTTTGAGTAT AACCCACCGA TGTTCGAAAT 3481 CAGCAAGACG CACGGTGCGC GCACGTGGCT GCTGGATCCA CGTGCGCCGA AGGTCGAAAA 3541 GCCGAAAATG CTGGAGAATC TGAAAGAAGC CGTCAGCGAA GCGAAAGTTG GTGAGTGAAG 3601 AGCTTAGAGA CCGGTCTTGT GCGTGACTAT CT //