LOCUS       Exported     3917 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3917
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3883
                     /note=""
                     /translation="MAKESIIKVRDLLIEYGHGKNKVSAVKEVSFDIYKGETFGLVGESGSGKSTIGKALMGIEPINDGTIYYQDTLAFGKAPNLIKMNEKMEHHIKVMKLNQTAITKALEIYSEEYKRVYFKYIEGKYYDLKSKETVEYSDNKIRKIEEGLDLKDHKLVSKSKDPKLKLVEDAVREIIKRILKLYKLQDKSLAFLKMLKEDGIINPELLKKVNELHSKTNKLTVKIRKSESKMYLTIQEIEKVRNDVRKGKYKSVKRFFFDLGKLLEVLIAEHKLVSPMISDLKQAQKLSSAIVSTGSDKKQWLKWIDEKIATIDDKSKVAEFELIKDLMSIESIQKTLEKSPKYSLPTSSERHELKKQMQMIFQDPASSLNDRMPVEEIIAEGLDNFPELYKNEQAAQTYIDWFNSNNPDKVGKITLENIRYQKVKQFLILQLLTTVGMLPEHLSRYPHEFSGGQRQRIGIARALVMKPSFVVCDEPISALDVSIRAQVINLLAKFQLEFDLTYIFIAHDLSVVRFIANRIAVIYRGDIVELADADELFNNPLHPYTKSLLSAVPLPDPDLEKKKKSIKYKPEEQHFDYITDSPKWIEVQKGHFILANEREVSAIKSEKRKIKKIEKGA"
                     /label="Mfl097"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGATATG TAGACACCGC CGGTCTCAAA TGGCCAAAGA
     2041 AAGCATTATC AAAGTCCGTG ATCTGCTGAT CGAATATGGT CATGGCAAAA ACAAAGTGAG
     2101 CGCCGTAAAG GAAGTCTCGT TTGACATTTA CAAAGGCGAA ACCTTCGGTC TGGTCGGCGA
     2161 ATCCGGCAGC GGTAAATCTA CCATCGGTAA AGCGCTGATG GGTATTGAAC CGATTAACGA
     2221 TGGTACCATC TACTACCAGG ATACGCTGGC GTTTGGCAAG GCACCGAACC TGATTAAAAT
     2281 GAACGAAAAA ATGGAGCACC ACATCAAAGT TATGAAACTG AACCAGACCG CCATTACGAA
     2341 AGCTTTGGAG ATTTACAGCG AGGAATATAA ACGTGTCTAT TTCAAATATA TTGAGGGCAA
     2401 GTACTACGAC TTAAAGAGCA AAGAAACGGT TGAATATAGC GACAATAAGA TCCGCAAGAT
     2461 CGAGGAGGGC CTCGATCTGA AAGATCATAA GCTGGTTAGC AAAAGCAAAG ACCCGAAACT
     2521 TAAGTTGGTC GAGGATGCAG TGCGTGAAAT CATTAAACGT ATCCTGAAAT TATATAAGTT
     2581 ACAGGACAAA AGCCTGGCAT TCCTGAAAAT GCTGAAAGAG GACGGCATTA TTAATCCGGA
     2641 GCTGCTGAAA AAGGTCAACG AGCTGCACTC TAAAACCAAT AAGTTGACGG TGAAGATCCG
     2701 CAAAAGCGAG AGCAAGATGT ATCTGACCAT CCAAGAAATC GAGAAGGTGC GTAATGATGT
     2761 GCGCAAGGGT AAGTATAAGT CCGTCAAACG CTTCTTTTTT GATCTGGGTA AACTGCTGGA
     2821 GGTGCTGATC GCGGAGCATA AGTTAGTTAG CCCGATGATT TCAGATTTAA AACAAGCTCA
     2881 GAAGCTGAGC AGCGCTATTG TTTCGACCGG TTCGGATAAA AAGCAATGGC TGAAATGGAT
     2941 CGACGAGAAG ATTGCAACGA TTGACGATAA GAGCAAAGTT GCAGAGTTTG AGCTGATTAA
     3001 AGATCTGATG AGCATTGAGA GCATTCAAAA AACGTTAGAG AAAAGCCCGA AATATAGCCT
     3061 GCCGACCAGC TCTGAACGTC ACGAACTGAA AAAGCAGATG CAGATGATTT TCCAAGACCC
     3121 GGCGAGCAGC CTGAATGACC GTATGCCGGT AGAAGAAATT ATCGCGGAGG GTCTGGACAA
     3181 CTTCCCGGAA CTGTACAAAA ATGAGCAGGC TGCCCAGACC TACATTGACT GGTTTAACTC
     3241 TAATAATCCG GACAAGGTCG GCAAGATTAC TCTGGAAAAT ATCCGTTACC AAAAGGTCAA
     3301 ACAATTTCTG ATCTTGCAGC TGCTGACGAC CGTTGGTATG TTGCCGGAGC ATCTGTCACG
     3361 TTACCCGCAT GAATTTAGCG GTGGTCAGCG TCAGCGTATC GGTATTGCGC GTGCGCTGGT
     3421 GATGAAGCCG AGCTTTGTTG TTTGCGACGA ACCGATCTCC GCACTGGACG TATCCATTCG
     3481 TGCGCAAGTA ATCAATCTGC TGGCGAAGTT CCAACTGGAA TTCGACCTGA CCTACATTTT
     3541 TATTGCGCAT GATCTGAGCG TGGTCCGTTT TATCGCTAAC CGTATTGCTG TGATCTATCG
     3601 CGGCGACATC GTCGAACTGG CCGATGCGGA CGAGCTGTTT AATAATCCGT TGCACCCGTA
     3661 CACCAAAAGC CTGCTGAGCG CAGTACCGTT GCCGGACCCT GACCTGGAGA AGAAGAAGAA
     3721 AAGCATTAAG TACAAGCCGG AAGAACAACA TTTCGATTAT ATTACTGATA GCCCAAAATG
     3781 GATCGAGGTT CAAAAGGGTC ATTTTATCCT GGCTAACGAG CGTGAAGTGT CCGCGATCAA
     3841 AAGCGAGAAA CGTAAGATTA AGAAGATTGA GAAGGGTGCC TGAAGAGCTT AGAGACCGTG
     3901 TTCTATGTCC CCATCGT
//