LOCUS Exported 5045 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..5045 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 2030..5011 /label="Mfl098" /note="" /translation="MKKLLSIIAAGTLVTTAGSTLVSCGMSTTKLMARKVNTKEYKGFMTAALNTWSPGSTMQNSDSIILENLYDGLLTPNAHGEIEGQMADWWGHNAKGTEYYFHLRDEEKTSEDGRKTGIPKWTAVRNGEIKSTQNVSTMDFYNAFRFTFNPNSSADGAAPTNGLFKNGTALEGILSTISEYDATVLGGANFSRTTAQGGEQNIGSEYISTRNFDIIVMLINLWTATPEGQTIINKLADAKLNPILDVNGNPTGKYDRTPEFDNLIAEMKKYQGETAFQKLISDSSQNGGMMAISLSSEGLGTAASSKGTYDTRTEQMYNIRYTLENASTSFFTSAAGYGSLKPLPDYAVSYSDNQNRSWYNFSKRYQPSLNEMWFSGAYYVQSYKASSNTILLKNPNYYQADRTYIEKLIFTLTRSQSVDSNRLWFEGGDASEVAISPNDASGWKKYVGDDYNSEEQKFAFEGTHATSTVPSQYTFTTFYNYGRLTNDGEDISKSSRALAQKSVRLYLNYFMQRTQYPAYVAGKLDNDKNTKESLAWDGGEGKNVKTRSSTLLRNTFTIPKLATNATESENGKVNGIPVEDYTIQNIGTDYNEMYGTKNTQLESSASSVEEETSTNASRLKTLEDNITVPYDKELLENFYNEQFGSLIDGNDAFYQNDLMALGMFLDSEGKVIDGLQTDLNNFANLAYTSIVDRSKNSKELDQNNEKAKAKILGDVVRKDLRSKQILTSDTDSISLEWLLNGALRLTLNPRVEYLVDQFNATVGGSSPITLKTKTSNDVSDYLNLSKIGDFDIYVSGWGPDYTDPYNFLHTLIWGGEYGVYTNIGNVLESDSSAKTESVKDENPNEPNLKIKDSFKQYEKDYAEINQSITKYTGVVETAKGQGDFTERLKTLSKAETYALYDVALTTPLYNRTPMKTIQLSYLDPFTRSSFIAGSSNLRLFGVKMIESLWDKDAFLAAQTQFEKGSSNEVEEYKKLYVYDRNSDGEVIATPRNK" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GACAAGGAGT CGGCATATCA CGGTCTCAAA TGAAGAAACT 2041 GCTGTCCATT ATCGCGGCTG GTACTTTGGT TACCACGGCG GGTTCCACGC TGGTGAGCTG 2101 TGGCATGAGC ACCACGAAGC TGATGGCGCG TAAGGTTAAT ACCAAAGAGT ATAAAGGTTT 2161 CATGACGGCA GCCCTGAATA CCTGGAGCCC GGGCAGCACC ATGCAAAACA GCGACAGCAT 2221 TATTCTGGAA AATCTGTATG ACGGCCTCCT GACCCCGAAT GCACACGGCG AAATTGAAGG 2281 TCAGATGGCC GACTGGTGGG GTCACAACGC AAAAGGCACG GAGTACTACT TCCATCTGCG 2341 TGACGAAGAG AAAACCAGCG AGGACGGTCG TAAAACCGGT ATTCCGAAAT GGACCGCGGT 2401 TCGCAACGGC GAGATCAAAA GCACCCAGAA TGTGAGCACC ATGGACTTTT ATAATGCTTT 2461 CCGTTTCACC TTTAACCCGA ACAGCAGCGC AGACGGCGCC GCTCCTACCA ACGGTCTGTT 2521 TAAAAACGGC ACTGCGCTGG AGGGTATTCT GAGCACCATC AGCGAATACG ACGCGACCGT 2581 GTTGGGCGGT GCTAACTTCT CTCGTACCAC GGCGCAGGGC GGTGAGCAGA ATATTGGTTC 2641 CGAGTACATT AGCACTCGTA ATTTTGATAT TATCGTCATG CTGATTAACC TGTGGACCGC 2701 AACGCCGGAG GGTCAGACCA TCATCAACAA GCTGGCAGAT GCGAAACTGA ACCCGATTTT 2761 GGACGTCAAT GGTAACCCGA CGGGCAAGTA CGATCGCACC CCTGAGTTCG ATAACCTGAT 2821 TGCAGAAATG AAAAAATACC AGGGCGAAAC GGCATTCCAG AAACTGATTA GCGACTCTTC 2881 CCAAAACGGC GGTATGATGG CCATTTCTCT GTCGTCTGAA GGCCTGGGTA CCGCCGCGAG 2941 CAGCAAAGGT ACGTATGACA CGCGCACCGA GCAAATGTAC AACATTCGTT ATACTCTGGA 3001 AAATGCGTCT ACCTCCTTCT TCACGTCAGC CGCTGGCTAC GGCAGCTTGA AGCCGCTGCC 3061 GGACTACGCT GTCTCGTACT CTGACAATCA AAACCGTTCC TGGTACAACT TCTCTAAACG 3121 TTACCAGCCG AGCCTGAACG AAATGTGGTT TAGCGGCGCC TACTACGTTC AGAGCTACAA 3181 GGCGTCCAGC AACACGATCC TGCTGAAGAA CCCGAATTAC TATCAAGCCG ATCGTACCTA 3241 TATTGAGAAG CTGATTTTCA CCCTGACGCG TAGCCAGAGC GTGGACTCGA ATCGTCTGTG 3301 GTTTGAAGGC GGTGATGCAA GCGAAGTTGC AATTTCGCCG AACGACGCGT CTGGCTGGAA 3361 GAAGTATGTT GGTGATGATT ACAATAGCGA GGAACAAAAA TTTGCCTTTG AAGGCACCCA 3421 CGCAACGAGC ACCGTTCCGA GCCAATACAC CTTTACGACC TTCTACAATT ACGGTCGCCT 3481 GACGAACGAT GGTGAGGACA TTAGCAAAAG CAGCCGCGCA CTGGCCCAAA AGAGCGTCCG 3541 CCTGTACTTG AATTATTTCA TGCAGCGCAC CCAGTACCCG GCTTACGTCG CCGGTAAGCT 3601 GGACAACGAT AAGAACACCA AGGAAAGCCT GGCCTGGGAC GGCGGCGAGG GTAAAAACGT 3661 TAAAACCCGT TCCAGCACCC TGCTGCGTAA TACCTTCACC ATCCCGAAGT TGGCCACTAA 3721 TGCCACGGAA AGCGAAAACG GTAAAGTTAA CGGCATTCCA GTGGAGGACT ATACGATTCA 3781 AAATATCGGC ACCGACTATA ACGAGATGTA TGGTACCAAA AACACCCAAC TGGAGAGCAG 3841 CGCGAGCTCC GTGGAGGAAG AAACCTCTAC GAACGCAAGC CGTCTTAAGA CGCTGGAGGA 3901 CAACATTACT GTGCCGTACG ATAAGGAACT GTTGGAAAAT TTCTATAATG AGCAATTCGG 3961 CAGCCTGATT GATGGTAATG ATGCATTTTA CCAGAATGAC TTGATGGCAC TGGGTATGTT 4021 TCTGGACAGC GAAGGCAAAG TAATTGACGG TCTGCAAACC GATCTGAATA ATTTTGCGAA 4081 CCTGGCATAC ACCAGCATTG TTGACCGTTC GAAAAACTCT AAAGAGCTGG ACCAAAATAA 4141 TGAGAAGGCC AAAGCAAAGA TCCTGGGTGA CGTTGTCCGT AAGGATCTGC GCTCCAAACA 4201 GATCCTGACG TCCGATACCG ACAGCATCTC TCTGGAGTGG CTGCTGAATG GCGCGCTGCG 4261 CCTTACGTTG AATCCGCGCG TGGAGTACCT GGTGGATCAG TTTAATGCTA CGGTGGGCGG 4321 TTCTAGCCCG ATTACCTTAA AGACCAAAAC GTCTAACGAT GTCTCCGACT ATCTCAACCT 4381 GAGCAAAATT GGTGATTTCG ATATTTATGT GAGCGGCTGG GGTCCGGACT ACACGGATCC 4441 GTATAACTTT CTGCACACCC TTATTTGGGG TGGCGAATAC GGCGTTTATA CGAACATTGG 4501 TAACGTCCTG GAAAGCGATT CTAGCGCCAA GACGGAATCC GTCAAGGACG AAAACCCGAA 4561 CGAGCCGAAT TTGAAGATCA AAGACAGCTT CAAACAGTAT GAAAAAGACT ATGCCGAAAT 4621 TAACCAAAGC ATCACCAAGT ACACCGGTGT TGTTGAAACT GCCAAAGGTC AGGGCGACTT 4681 CACGGAGCGC CTCAAGACCT TGAGCAAGGC GGAAACGTAC GCTCTGTACG ACGTGGCGCT 4741 GACCACCCCG CTGTATAATC GTACCCCGAT GAAAACCATC CAGCTGTCTT ACTTGGATCC 4801 GTTCACGCGC AGCTCTTTCA TTGCGGGTAG CTCGAATCTG CGTCTGTTTG GTGTGAAGAT 4861 GATCGAATCA CTGTGGGATA AGGATGCCTT CTTGGCGGCC CAAACCCAAT TCGAGAAAGG 4921 TAGCAGCAAT GAAGTTGAAG AATACAAAAA GTTGTACGTT TACGACAGAA ATTCCGATGG 4981 TGAAGTTATT GCGACCCCGC GCAATAAGTG AAGAGCTTAG AGACCGGACC TTGACGCCTA 5041 ATACT //