LOCUS Exported 2992 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2992 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1030..1077 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2925..2941 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2942..2973 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..958 /label="Mfl099" /note="" /translation="MKERIINSLDDNKISIYIWDEVKNPKAIIQLVYGSCEHSLRYKEFAKKMNDNQIIVISSDHRGHGKTALLNNKPLGYFSKNNGWNIIISDLKQVNSFIKNQYMNLPIIMLGHSMGSFMARTYMIDYPNTVDAYVISGTAWHNKALLIFSKNIAHIRSKINGGKKPDNFIWKLSYKPLNKKFNSKKATGFEWLSNDSINKNEFILDPLTGQVFSSSAFKDMFYGLTYIQKKSNIKKIKKSTPILLISGKEDPVGNYGKMVLKTNKIFRQNNLNVKVNLYENQRHEILFDEKKSKVEKDIIVFINETLQNK" primer_bind complement(1082..1098) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1165..1753) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1944..2804) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2805..2909) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGGCCCAGA AGGATACAAC GGTCTCAAAT GAAAGAACGT ATCATTAATA GCCTGGATGA 61 TAACAAGATC AGCATTTACA TCTGGGACGA AGTCAAAAAC CCGAAGGCTA TCATCCAGTT 121 GGTCTACGGC AGCTGCGAAC ATAGCTTGCG CTACAAAGAG TTCGCGAAAA AGATGAATGA 181 CAATCAGATC ATCGTCATCT CCAGCGACCA TCGTGGTCAC GGCAAAACGG CACTGCTGAA 241 CAACAAGCCG CTGGGTTACT TCAGCAAAAA CAACGGTTGG AACATTATTA TCAGCGATCT 301 GAAGCAAGTA AACTCTTTTA TTAAAAATCA GTATATGAAC CTTCCGATTA TTATGCTGGG 361 CCACAGCATG GGTTCCTTCA TGGCGCGTAC CTATATGATT GACTACCCGA ATACCGTGGA 421 TGCATATGTG ATCAGCGGTA CGGCATGGCA TAATAAAGCC CTGCTGATCT TCAGCAAAAA 481 TATCGCCCAT ATTCGTAGCA AAATTAATGG TGGTAAGAAA CCGGACAATT TTATTTGGAA 541 GTTATCTTAC AAGCCGCTGA ACAAAAAGTT CAATTCAAAG AAGGCGACGG GTTTCGAATG 601 GCTGAGCAAT GATAGCATTA ATAAGAACGA GTTTATTCTC GATCCGCTGA CGGGCCAGGT 661 ATTCTCTAGC AGCGCATTTA AGGATATGTT TTACGGTCTG ACGTACATCC AGAAAAAGAG 721 CAATATTAAG AAAATCAAAA AGAGCACGCC GATTCTGCTC ATTAGCGGCA AGGAGGATCC 781 GGTTGGTAAT TACGGCAAAA TGGTTCTGAA AACTAATAAG ATCTTCCGTC AAAATAACCT 841 CAACGTTAAG GTGAACCTGT ATGAGAATCA ACGTCACGAG ATTTTGTTTG ATGAGAAGAA 901 AAGCAAGGTT GAAAAAGATA TCATTGTTTT CATCAATGAA ACCTTGCAAA ACAAGTGAAG 961 AGCTTAGAGA CCGTTACACC GGGATCATAG CTCGCTGAGG TGTCAATCGT CGGAGCCGCT 1021 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT 1081 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG 1141 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 1201 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 1261 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 1321 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 1381 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 1441 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 1501 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 1561 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 1621 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 1681 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 1741 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG 1801 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 1861 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 1921 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 1981 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA 2041 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG 2101 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 2161 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT 2221 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 2281 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA 2341 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 2401 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 2461 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA 2521 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA 2581 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA 2641 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 2701 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 2761 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA 2821 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 2881 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG 2941 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG //