LOCUS       Exported     2614 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2614
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      652..699
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2547..2563
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2564..2595
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..580
                     /label="Mfl112"
                     /note="This protein is part of the stalk that links CF(0) to CF(1). It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction."
                     /translation="MVLKDNVIDNWANALTKIAVKENKVKKMLEQAHVLIEVLKNKNEFVDILTFKSAHDEEKRIKIIDDTFSQFNIDEDIMNAFKILVHMQAFVNARDILKKLRGKLVELDNMTYGVVWSTEEISAAQIKEIEEKMSKKINKEVKLVNKIDTKLIAGIQVVVHNKVYDGSLRSKLDEMKYQVLKEK"
     primer_bind     complement(704..720)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(787..1375)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1566..2426)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2427..2531)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAACGAGCA GACCGAATAG GGTCTCAAAT GGTCCTGAAA GATAACGTGA TTGACAACTG
       61 GGCGAACGCT CTGACCAAAA TCGCGGTCAA AGAGAACAAA GTTAAGAAAA TGTTGGAACA
      121 AGCCCACGTT CTGATTGAAG TGCTGAAAAA TAAGAATGAA TTCGTTGACA TTCTGACCTT
      181 CAAGTCGGCG CATGACGAGG AGAAACGTAT TAAAATTATT GATGACACCT TCAGCCAGTT
      241 CAATATCGAC GAGGACATTA TGAACGCGTT CAAGATTCTG GTTCACATGC AGGCATTCGT
      301 TAACGCCCGT GATATCCTGA AGAAGTTGCG TGGCAAACTG GTGGAGCTGG ACAACATGAC
      361 CTATGGCGTG GTTTGGTCCA CGGAGGAGAT TTCGGCGGCA CAAATCAAAG AAATCGAAGA
      421 AAAAATGTCA AAAAAGATTA ATAAGGAGGT TAAACTGGTC AACAAAATTG ACACCAAACT
      481 GATCGCGGGT ATCCAGGTGG TGGTTCACAA TAAAGTTTAT GACGGTTCGC TGCGTAGCAA
      541 GCTGGATGAA ATGAAGTATC AAGTTCTGAA GGAAAAGTGA AGAGCTTAGA GACCCAGCCT
      601 CTGTGTTGTC TGATCGCTGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
      661 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
      721 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
      781 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
      841 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
      901 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
      961 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     1021 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     1081 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     1141 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     1201 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     1261 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     1321 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGG
     1381 CCTACTATTA GCAACAACGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     1441 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
     1501 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     1561 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
     1621 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
     1681 GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA
     1741 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
     1801 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
     1861 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
     1921 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
     1981 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
     2041 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
     2101 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
     2161 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
     2221 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
     2281 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
     2341 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
     2401 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
     2461 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
     2521 GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC
     2581 CGGAGGCTTT TGACTTGGTC AGGTGGAGTG GGAG
//