LOCUS Exported 3641 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3641 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3607 /label="Mfl113" /note="Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit." /translation="MALNIKEISEVIEKQIKNYGKDIIEAEQGSVVTIGDGVSLIYGLDKALMGELLIFPNDVYGMVLSLEEGAVGAVILGDYKLIKEGDIVKRTGKVVETPVGDAMIGRVVNALGQPIDNNGPIKTKKSKPVERIATGVMARKSVSQPLETGILGIDASIPIGKGQRELIIGDRQTGKTAVAIDTIINQKGKNVKCIYVSIGQKDSTIAQVVEKLKKFGAMEYTTVVNAGASDSAPLQYLAPYTGVTIGEEWMENGEDVLIVYDDLSKHAVAYREMSLLLRRPPGREAYPGDVFYLHSRLLERAARVNEKFGGGSITALPIIETQASDISAYIPTNVISITDGQIFLSSDLFMAGIRPAINIGPSVSRVGSSAQIKAVKQVSGTLKLELAQYYELEAFSKFGSDLDESTKATLDHGARIIQMLVQRQYSPLNQIDEAIILFAIKSHLIKWIPLENIRDFKTEIITFFNNEKDAKALKAELTKKLEWNADLESGIQKEIEKLVVKFTSTLKNYNPTIFGDEKEFKKLGK" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAGAATTAC TGACCCCTCG GGGTCTCAAA TGGCGCTGAA 2041 TATTAAGGAG ATCAGCGAGG TTATCGAAAA ACAAATCAAG AATTACGGTA AGGACATCAT 2101 TGAAGCGGAG CAAGGTAGCG TGGTCACCAT TGGCGACGGT GTGTCCCTGA TCTACGGCCT 2161 GGATAAGGCG CTGATGGGTG AACTGCTGAT TTTTCCGAAT GATGTTTACG GCATGGTTCT 2221 GAGCCTGGAG GAAGGTGCGG TGGGTGCAGT AATCCTGGGT GATTACAAGT TGATTAAGGA 2281 AGGCGACATT GTTAAGCGCA CCGGCAAAGT GGTCGAAACC CCGGTGGGTG ATGCCATGAT 2341 CGGTCGTGTC GTGAATGCGC TGGGTCAACC GATTGATAAC AATGGTCCGA TTAAGACCAA 2401 GAAAAGCAAG CCTGTGGAGC GTATTGCGAC CGGCGTGATG GCTCGTAAGA GCGTTAGCCA 2461 ACCGCTGGAA ACCGGTATTC TGGGTATTGA TGCCTCCATC CCGATCGGTA AGGGTCAAAG 2521 AGAACTGATC ATCGGTGATC GTCAAACGGG TAAAACGGCC GTTGCAATTG ACACCATTAT 2581 TAACCAGAAG GGTAAAAATG TCAAGTGTAT TTACGTTAGC ATCGGCCAAA AAGATTCTAC 2641 GATCGCGCAA GTCGTTGAAA AGCTGAAAAA ATTTGGCGCA ATGGAGTATA CGACCGTGGT 2701 TAACGCCGGT GCGAGCGACA GCGCGCCATT GCAGTATCTG GCGCCGTATA CGGGTGTTAC 2761 GATTGGTGAA GAATGGATGG AGAATGGTGA AGATGTTCTG ATCGTTTACG ACGACCTGAG 2821 CAAACACGCA GTTGCATATC GTGAAATGAG CCTGCTGCTG CGTCGCCCGC CTGGCCGCGA 2881 GGCTTATCCG GGTGATGTCT TTTACCTCCA CAGCCGTCTG CTGGAGCGTG CGGCACGCGT 2941 CAACGAGAAA TTCGGCGGTG GTAGCATTAC CGCACTCCCG ATCATCGAAA CCCAAGCAAG 3001 CGACATTTCC GCATACATCC CGACCAACGT GATCAGCATC ACTGACGGCC AGATCTTTTT 3061 GAGCAGCGAC CTGTTCATGG CGGGCATTCG TCCGGCAATT AACATCGGTC CGTCCGTTAG 3121 CCGCGTTGGT AGCTCTGCGC AGATCAAGGC GGTGAAACAA GTCTCCGGTA CCCTGAAGCT 3181 GGAGCTGGCC CAGTACTATG AGCTGGAGGC TTTCTCTAAG TTTGGCTCTG ATTTGGACGA 3241 GAGCACCAAA GCAACCCTGG ACCACGGTGC GAGAATCATT CAAATGCTTG TGCAGCGCCA 3301 ATACAGCCCG CTTAATCAAA TTGATGAGGC GATCATTCTG TTTGCCATCA AAAGCCACCT 3361 GATTAAGTGG ATCCCGCTGG AAAACATCCG TGATTTTAAA ACCGAAATCA TTACGTTCTT 3421 CAATAACGAA AAGGACGCCA AGGCTCTGAA AGCAGAACTG ACCAAGAAGC TGGAGTGGAA 3481 CGCCGACCTG GAGTCTGGTA TTCAGAAAGA GATCGAGAAG CTGGTCGTCA AATTCACGAG 3541 CACCCTGAAG AACTACAACC CGACCATTTT TGGCGACGAA AAAGAGTTTA AAAAGCTGGG 3601 TAAGTGAAGA GCTTAGAGAC CCGACTGCCG ACATGATCTA T //