LOCUS Exported 2788 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2788 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 826..873 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2721..2737 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 2738..2769 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..754 /label="rpsC" /note="Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation." /translation="MGQKVSPNVLRLGIVRDWEDTWYAEKDQYVKWLDQDIKIREGVLKLLKDAAVSKIKIERTNSNITLIIRTARPAIVLGQEGKNVSNIATAVQKIAKDRNLKVEVKVIEVKNPDADATLVARWIGEQITNRASFRTVQKLAIRKALKAGVKGIKTSVSGRLGGVEMARTEGYIEGSVPLSTLRADIDYALYEAPTTYGQIGVKVWINHGEVIGGQSQRVSEKAPMNNDRRFNNKNNNRGGRK" primer_bind complement(878..894) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(961..1549) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1740..2600) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2601..2705) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 AAGGAACTAT GGCATCGAGC GGTCTCAAAT GGGCCAGAAG GTTAGCCCTA ATGTTCTTCG 61 CCTGGGCATT GTTCGCGATT GGGAAGATAC TTGGTATGCA GAGAAGGACC AATACGTCAA 121 GTGGCTGGAC CAGGATATTA AGATTCGTGA AGGTGTTCTG AAACTGCTGA AAGACGCAGC 181 GGTGTCCAAA ATCAAGATTG AACGTACCAA TAGCAACATC ACCTTGATTA TCCGTACTGC 241 TCGCCCGGCC ATCGTCCTGG GTCAGGAAGG CAAAAACGTT AGCAATATCG CAACTGCGGT 301 TCAGAAAATC GCAAAGGATC GCAACCTGAA AGTTGAGGTC AAGGTGATTG AGGTGAAGAA 361 CCCGGATGCG GATGCGACGT TGGTGGCACG TTGGATTGGT GAGCAAATTA CCAACCGTGC 421 CTCTTTCCGT ACGGTCCAAA AACTGGCGAT TCGCAAAGCT CTGAAAGCGG GTGTCAAGGG 481 TATCAAAACG AGCGTTAGCG GTCGTCTGGG CGGTGTTGAG ATGGCGCGTA CTGAGGGCTA 541 TATTGAGGGT AGCGTGCCGC TGAGCACCCT GCGCGCCGAT ATCGATTACG CGCTGTACGA 601 AGCGCCGACC ACCTATGGCC AAATTGGCGT CAAAGTCTGG ATTAACCATG GCGAGGTTAT 661 CGGCGGCCAG AGCCAGCGCG TTAGCGAGAA AGCCCCGATG AACAACGATC GTCGTTTCAA 721 CAATAAAAAC AACAACCGCG GCGGCCGTAA GTGAAGAGCT TAGAGACCCG CATGTAGGCA 781 CCATCTATCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT 841 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT 901 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA 961 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 1021 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 1081 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 1141 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 1201 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 1261 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 1321 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 1381 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 1441 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 1501 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT 1561 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 1621 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 1681 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT 1741 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA 1801 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC 1861 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC 1921 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG 1981 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC 2041 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC 2101 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG 2161 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC 2221 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT 2281 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC 2341 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC 2401 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC 2461 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC 2521 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA 2581 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 2641 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 2701 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG 2761 CTTTTGACTT GGTCAGGTGG AGTGGGAG //