LOCUS       Exported     3524 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3524
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3490
                     /label="secY"
                     /note="The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently."
                     /translation="MAKKFAKKSKQYSAKKSQSNNSDLRTGNFFIKNKDILGRIAFTLLLLVIIRIGVYITVPGIRLTSDYQNAINNSQFFQLLSTLGGGTIGRFSILALGVSPYITASIIVQLLSTDVVPILTRWNKSGERGRKKLDKLTKVLMIPFALMQGIATIFTLQQQGVIEPGWSSDNVLASPAFYYVLVPLVMLAGSYFMLWIADQITIKGVGNGISIVIFIGIIVQLPNQIKATYDFWIPSNESINVFFDGIIKFSIYMLVFFVVIFSVVLMNEAERKVPIQQTGSGLIDSKDHTPYLPLKLNNAGVIPVIFASALISTPITIAQIIDPTASTSTVDSANAFVRFTQHYLSFNTWWGIGIFAVMIVLFTFLYAQVQINPEKISENFQKSGTFIPGIKPGKDTTNFLKGTINRLSLFGAIFLAAIAALPYVISKLTNLPSQLAIGGTGLIICISVAIQTTQQLQGRITQHKFIESKKQNFTEEASTNSSSHIW"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGCGCTC GGATAATACT CGGTCTCAAA TGGCCAAAAA
     2041 ATTTGCAAAG AAAAGCAAGC AGTACAGCGC GAAGAAAAGC CAAAGCAATA ACAGCGATCT
     2101 GCGCACTGGC AATTTCTTCA TTAAAAATAA AGACATCCTG GGCCGTATCG CATTTACGCT
     2161 GCTGTTGCTG GTCATCATCC GTATTGGTGT TTACATCACT GTGCCGGGTA TTCGCCTGAC
     2221 CAGCGATTAC CAAAATGCGA TCAACAATAG CCAGTTCTTC CAGCTGCTCT CGACCCTTGG
     2281 TGGTGGCACC ATTGGCCGCT TCAGCATTCT GGCATTGGGT GTGTCCCCGT ACATCACCGC
     2341 GAGCATTATC GTCCAGTTGT TGAGCACGGA CGTGGTGCCG ATCCTGACCC GTTGGAATAA
     2401 AAGCGGTGAG CGTGGTCGTA AGAAATTGGA CAAACTGACG AAGGTGCTGA TGATCCCGTT
     2461 TGCCCTGATG CAAGGCATTG CGACCATTTT TACCCTGCAA CAGCAGGGCG TGATCGAGCC
     2521 GGGTTGGAGC TCCGACAACG TTTTGGCGAG CCCGGCGTTT TACTATGTTC TGGTGCCGCT
     2581 GGTCATGCTG GCCGGCTCTT ATTTCATGCT GTGGATCGCG GATCAAATCA CCATCAAGGG
     2641 CGTCGGCAAC GGTATTAGCA TCGTGATTTT CATCGGCATT ATTGTGCAAT TGCCTAATCA
     2701 GATCAAAGCC ACGTACGACT TTTGGATCCC GTCCAATGAA TCTATTAACG TTTTCTTTGA
     2761 CGGTATTATC AAATTCAGCA TTTACATGTT GGTGTTTTTC GTGGTTATTT TCAGCGTAGT
     2821 TCTGATGAAC GAAGCGGAGC GTAAAGTTCC GATCCAACAG ACCGGTAGCG GTCTGATTGA
     2881 TTCTAAGGAC CATACCCCGT ACCTCCCGCT GAAACTGAAC AACGCCGGTG TTATTCCGGT
     2941 TATTTTTGCT AGCGCTCTGA TTAGCACGCC GATTACCATT GCTCAGATCA TCGACCCGAC
     3001 GGCCAGCACT TCTACCGTCG ATAGCGCAAA CGCGTTTGTG CGTTTTACCC AACATTACCT
     3061 GAGCTTCAAC ACCTGGTGGG GTATTGGTAT TTTTGCCGTC ATGATTGTCC TCTTTACGTT
     3121 CCTGTATGCC CAAGTGCAAA TCAACCCGGA GAAGATCAGC GAAAATTTCC AGAAAAGCGG
     3181 TACCTTTATT CCGGGCATTA AGCCGGGTAA GGATACCACT AATTTTCTGA AGGGTACGAT
     3241 TAACCGCCTC AGCCTGTTTG GTGCGATTTT CTTGGCTGCG ATTGCGGCGC TGCCGTACGT
     3301 CATTAGCAAA CTGACGAATC TGCCGTCCCA GCTGGCAATT GGCGGTACTG GTCTGATTAT
     3361 CTGCATCTCC GTGGCGATTC AAACCACGCA GCAACTGCAA GGTCGCATTA CCCAACACAA
     3421 GTTTATTGAG AGCAAGAAGC AAAATTTTAC CGAAGAGGCG AGCACCAATT CCAGCAGCCA
     3481 CATCTGGTGA AGAGCTTAGA GACCCCATCT TGCGTCGTGA GAAT
//