LOCUS       Exported     6353 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..6353
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..6319
                     /translation="MHFKKIYLMLKNSFKNATKSKTQLIGVTVLAFLLSLVLTLVVSMNVRVIEKYKEMNENSRVHDAVIDLNPYDKVATGESEETEEAPKNLVAAQQYWIYKLQEKYFEESSDLQFEWSRTEAREFSQVKQNGNDLTIKAITKTSQENNFSNQEVDKLVIFEGEDIQTHHQVVIDPNYAKNNGIKLGDVIRIQADNLGNSLLVRDSDNKQVASDIKEIEATGTEIDAIDGKYNIYYSNYQWFQVVGFGSSADFMSPIFNASTTLPSRNKEVMIYVNPTAFGLKYNQESNLYEYNLNQNGNLTVSSNVEIESFYSIKFKDAKKAKSTGLEQFESDLKELIRRNSNNKIVYGKEDSSYRFSKRILLIQKTIRTYNIAAFVIFILILFVCLYTISLVTKKQIEKASKQLGTLKALGYRKRILVYNFVMLPVIASTIGGLLGYIVSISVSNTLTNEFASYFSLNYSKFDFDWITLVLMIGVMWFILSAISFVIATMLMRKSSLSLITATFNQKASAFKAKLRSIHFRKSFGSKLRKALLVDAFGKMMAIGFVVLLSSMLFTVSFAAPDILKRNEKATYTGVKYKQIVEYAQPSYNNPLTFAKTFNPSTKQDEMVYSKTAGGWTSLALRDNGDFDYDQIMTDYFNNEISEKYYSIFIQNLFTASSDDEYAIPNLIELSLANMKLLNLEGSIFDSNYFRQLSKYGVPAASNSDILGKMISPIILKQWFDYQNLFSEINSAKTMYEAGAAMQTFYAKYSESIGLSISNDFRSDYGTKKISDEEWIKMSEHSKINVFNSSNGNLANMYLLNNEDMLKTLLKAETGKIEYENQTNQNNKFKLTNSNYTGIGSFRIASEHNTEESTNDYYLGLNFDKMAEENDKDKAIEAITDMWEWFTFLFNNRVDQAIIQSAFSRPPYSVKQTLINAFKSNDKNYSMAFNLVSYDPSQEALGTKIQAEKDGKNFKIYGIDNDDRFLDLRDSNNNNLMEELFNFKETNGIVINETLAKTLNLKEGQEVDFNVIQNELQDASKGEIVPYKLNDWDTSSLWNGTDGFKQNSRTNNLGSNILVTRPDLDDKNAINFMTSISKPTEYYNSVLNGDTIIADKTTNTKFKIVGIHEGYGSAQAWIKNDDAKSILKYDEVENYLWKNFFAKQWNTQFGYSTLDQFNNKIIIEDKDQEELKIYNKIKGLDLTKNGLDDLDLFKNKFIEHPQNNDEKALGELILKIFENQYPVFNYKYSNKTDVADYNTVMSVSTAFGDYSPTALNGMEAKFSATHAAFDGSGIGTVEWILPIDLSKDMLEEISQLILLLIAIAIVLILSLTFVIILLTTSIIITDNIRFISTMRVLGYHDAYVVKTVMGMYLIVISTMFAVGFAAGWFIFAKAVNIMLLSGVVLPIAFPIWLPIVVFLGIVGIYAIAIFAGYKRITKTNSVQILQNADL"
                     /label="Mfl156"
                     /note=""
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATATCACG CAAAAGCACC GGGTCTCAAA TGCACTTCAA
     2041 GAAAATCTAT CTGATGCTGA AGAATTCCTT TAAAAACGCG ACGAAATCTA AGACCCAATT
     2101 GATTGGTGTT ACGGTCCTGG CGTTCCTGCT GAGCCTGGTG TTGACCCTGG TTGTTAGCAT
     2161 GAACGTGCGT GTCATTGAGA AATATAAAGA GATGAATGAA AATAGCCGCG TGCACGACGC
     2221 CGTTATTGAT CTGAATCCGT ACGATAAAGT GGCGACCGGT GAGTCCGAGG AAACCGAAGA
     2281 GGCCCCGAAG AACCTGGTTG CGGCGCAACA ATACTGGATT TACAAACTGC AGGAAAAGTA
     2341 TTTCGAGGAA AGCAGCGACT TGCAGTTCGA ATGGTCGCGC ACCGAAGCGC GTGAGTTCAG
     2401 CCAGGTGAAG CAGAACGGCA ATGACCTGAC CATTAAGGCG ATTACCAAGA CCAGCCAGGA
     2461 GAATAATTTC AGCAACCAGG AGGTGGATAA ACTGGTCATC TTCGAAGGTG AGGACATTCA
     2521 GACCCACCAC CAGGTGGTCA TCGACCCGAA TTATGCTAAA AACAATGGTA TTAAACTGGG
     2581 TGATGTTATC CGTATTCAAG CAGATAATCT TGGCAATTCC CTGCTGGTTC GTGACTCCGA
     2641 TAATAAACAG GTCGCGAGCG ACATCAAGGA AATTGAGGCT ACGGGCACCG AGATTGACGC
     2701 AATCGATGGC AAATACAATA TCTATTATAG CAACTATCAG TGGTTTCAAG TCGTTGGTTT
     2761 TGGCAGCAGC GCGGACTTCA TGTCACCGAT CTTCAACGCG TCCACGACCC TGCCGAGCCG
     2821 CAATAAAGAA GTTATGATTT ATGTTAACCC TACCGCGTTT GGTCTGAAAT ATAATCAAGA
     2881 AAGCAATCTG TATGAATACA ACCTGAACCA AAATGGTAAT TTGACCGTTA GCAGCAATGT
     2941 TGAAATTGAA AGCTTCTACA GCATCAAGTT CAAGGATGCG AAGAAGGCGA AAAGCACCGG
     3001 CCTGGAGCAG TTCGAGTCCG ACCTGAAAGA GCTGATTCGT CGTAACAGCA ACAACAAGAT
     3061 CGTTTATGGC AAGGAAGATA GCTCCTATCG TTTTAGCAAG CGTATTCTGC TGATCCAAAA
     3121 GACCATCCGC ACGTATAATA TTGCCGCGTT TGTCATTTTC ATTCTGATTC TGTTCGTCTG
     3181 CCTGTATACG ATCAGCTTGG TTACCAAGAA ACAAATCGAA AAGGCTAGCA AACAGCTGGG
     3241 CACGCTGAAG GCTTTGGGTT ATCGTAAGCG TATTTTGGTG TACAACTTCG TTATGCTGCC
     3301 GGTGATCGCG AGCACCATCG GTGGTCTGCT GGGTTATATT GTCAGCATTT CGGTCAGCAA
     3361 CACGTTGACC AATGAGTTCG CGAGCTACTT TTCCCTGAAT TACAGCAAGT TCGACTTTGA
     3421 CTGGATCACG CTGGTTCTGA TGATCGGCGT TATGTGGTTT ATCCTGTCCG CAATCAGCTT
     3481 CGTCATTGCG ACCATGTTGA TGCGTAAATC GAGCCTGTCG CTGATTACCG CTACTTTCAA
     3541 CCAAAAAGCA TCCGCGTTTA AAGCGAAGTT GCGTAGCATC CACTTCCGCA AGAGCTTTGG
     3601 CAGCAAACTG CGCAAAGCTT TACTGGTCGA TGCGTTTGGC AAAATGATGG CGATCGGTTT
     3661 CGTCGTTCTG CTGAGCTCCA TGCTGTTCAC TGTCTCATTT GCAGCACCGG ACATCCTGAA
     3721 ACGTAACGAA AAGGCTACCT ATACGGGTGT GAAGTACAAA CAGATCGTGG AGTATGCGCA
     3781 GCCGTCCTAT AACAACCCGT TGACCTTTGC CAAAACTTTT AACCCGAGCA CCAAGCAGGA
     3841 TGAAATGGTT TACAGCAAGA CGGCGGGTGG CTGGACCAGC CTGGCGTTGC GTGACAACGG
     3901 CGACTTCGAT TACGACCAAA TCATGACCGA CTACTTCAAC AACGAGATTA GCGAAAAGTA
     3961 CTATAGCATC TTTATTCAAA ACCTGTTTAC GGCGTCCAGC GACGATGAGT ATGCGATCCC
     4021 GAATCTGATC GAGCTGAGCC TGGCGAACAT GAAGCTGCTG AACCTGGAGG GTTCCATTTT
     4081 TGACAGCAAC TATTTCCGCC AACTGAGCAA ATACGGTGTT CCAGCGGCAT CTAATAGCGA
     4141 TATTCTGGGT AAAATGATCT CTCCGATTAT CCTGAAGCAG TGGTTCGACT ATCAAAACCT
     4201 GTTTAGCGAA ATTAATAGCG CCAAAACCAT GTATGAAGCG GGTGCCGCTA TGCAAACGTT
     4261 TTACGCGAAG TACAGCGAGA GCATCGGTCT GAGCATCAGC AACGATTTTC GTAGCGATTA
     4321 TGGTACCAAG AAAATTTCCG ATGAGGAGTG GATTAAAATG TCTGAGCATT CGAAAATCAA
     4381 CGTTTTTAAT TCCTCCAACG GCAACCTGGC GAACATGTAT CTGCTGAATA ATGAGGACAT
     4441 GCTCAAGACT CTGCTGAAAG CGGAAACCGG TAAGATTGAA TACGAAAATC AGACCAATCA
     4501 GAATAACAAA TTTAAATTGA CGAACAGCAA TTACACTGGC ATCGGTTCGT TCCGTATCGC
     4561 GAGCGAGCAC AATACGGAGG AGAGCACGAA CGACTACTAC TTGGGTCTGA ACTTTGACAA
     4621 AATGGCTGAA GAGAACGACA AGGATAAAGC GATCGAAGCA ATCACGGACA TGTGGGAGTG
     4681 GTTCACGTTC CTGTTCAATA ATCGTGTGGA TCAGGCGATT ATTCAAAGCG CCTTTTCCCG
     4741 TCCTCCATAC AGCGTCAAGC AGACCCTGAT TAATGCGTTC AAAAGCAACG ATAAAAACTA
     4801 CAGCATGGCA TTCAACCTGG TTAGCTATGA TCCGAGCCAG GAGGCTCTGG GCACCAAAAT
     4861 CCAGGCCGAG AAGGACGGCA AGAATTTTAA AATTTATGGC ATTGACAACG ACGACCGTTT
     4921 TCTGGACCTT CGTGATAGCA ATAACAACAA CCTGATGGAA GAACTGTTTA ATTTCAAGGA
     4981 AACCAACGGC ATCGTCATCA ACGAAACCCT TGCTAAGACG CTGAACCTGA AGGAAGGTCA
     5041 AGAAGTTGAC TTCAACGTTA TTCAGAACGA ATTGCAGGAT GCGAGCAAAG GTGAGATTGT
     5101 GCCTTACAAG CTGAACGATT GGGACACGTC TAGCCTGTGG AACGGTACTG ATGGTTTTAA
     5161 GCAAAATAGC CGTACCAACA ATCTGGGCAG CAATATTTTG GTGACGCGCC CGGATCTGGA
     5221 TGACAAAAAT GCGATTAATT TCATGACCTC TATCTCTAAG CCGACCGAGT ACTATAATAG
     5281 CGTGCTGAAC GGCGACACCA TTATCGCAGA TAAAACCACG AACACCAAGT TCAAAATCGT
     5341 CGGCATCCAT GAGGGTTACG GTAGCGCACA GGCATGGATT AAGAATGATG ATGCAAAAAG
     5401 CATTTTAAAA TACGACGAAG TGGAAAACTA CTTGTGGAAG AATTTCTTCG CGAAGCAATG
     5461 GAATACCCAA TTCGGTTACA GCACGCTGGA TCAATTTAAC AATAAAATCA TCATTGAGGA
     5521 CAAGGATCAG GAAGAACTGA AAATTTACAA TAAAATTAAG GGTCTGGATC TGACTAAGAA
     5581 TGGCTTAGAT GACCTGGACC TGTTTAAAAA CAAGTTTATT GAGCATCCGC AGAACAACGA
     5641 TGAGAAAGCA CTCGGTGAGC TGATTCTGAA AATCTTCGAA AATCAATACC CGGTGTTTAA
     5701 TTATAAATAT AGCAACAAGA CGGACGTTGC TGATTATAAT ACTGTAATGA GCGTTAGCAC
     5761 TGCATTCGGT GACTATAGCC CGACGGCGCT GAACGGCATG GAGGCTAAGT TTAGCGCGAC
     5821 CCATGCCGCG TTTGACGGTA GCGGCATCGG TACGGTCGAA TGGATCCTGC CGATCGACCT
     5881 GAGCAAAGAC ATGCTGGAAG AGATCTCTCA ACTGATTCTG TTGCTGATCG CTATCGCCAT
     5941 TGTGCTGATT CTGAGCCTGA CCTTTGTGAT CATCCTGCTG ACCACGTCGA TTATTATCAC
     6001 GGACAATATT CGCTTTATTA GCACCATGCG TGTTCTGGGT TACCACGACG CTTATGTGGT
     6061 CAAAACGGTC ATGGGCATGT ACCTGATCGT TATCAGCACT ATGTTCGCAG TCGGTTTCGC
     6121 CGCTGGCTGG TTTATCTTCG CGAAGGCAGT TAACATCATG CTGCTGAGCG GTGTGGTGCT
     6181 GCCGATTGCA TTTCCGATCT GGCTGCCGAT CGTTGTTTTC CTGGGCATTG TTGGTATTTA
     6241 CGCAATTGCC ATCTTTGCGG GTTACAAACG TATCACCAAG ACGAATAGCG TTCAGATCCT
     6301 GCAGAATGCC GACCTGTGAA GAGCTTAGAG ACCGTGTAGT GTTCCCCATA CGT
//