LOCUS       Exported     4604 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4604
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4570
                     /translation="MKKILLSLSALTIGATPAVSLLNVGQKQVITQVTSEIQKKIDETSVLFKAPIITNAQKQNSDVTTNWLNDQKVSDISNINSALSYANFNAEKYDMKSVEKTLNKIYKDKDYKYKNEDATSELIFGAADKGVSLLENSIDDDGLKAEGVNELLNWISYVSFGTGLVGAVNAKSIDKSILNLNKNIEWIAAFKNTPIPTLSKLNNEYDVYTFQDLQDFIIISIGNLLSEASGFEGDSFAQSTGWGGRTKWNNIVKKELDLDTMKKVAIPQKNKNSSNVPKGQTSWDTTTNGNNREFSIYDERSIVQQETDFIQSIVKHIINSGNGLKIEMNSKNLQSIVNIVYSISWYISSYDKYSVYVMPSDDNYLFSETKSNMTIFNEQRTKTIEKTDLSNSLSGILSLFKSVFISKDRMTSNRGLKILFQNGDKTTNNWGSELSIFLSFIGGTWTRNNFNNGLNALLIGAMAGVTNSKLLDNVKSIDTILTLVGLNRNSLGYVIGNILNATLANTNSTNVFYGLATSTLVTIVGGIIPDVGKYLDEIKKMANDNAFDHTMTKLVSTKLNVLMDYLGNVMDVFSPPAETDPPREPSTLPNKSILEFMNMGVMGITVGNVIWSGLNFINTSLSEIMKPLGLLGALLSDDVSRNYNFYVKDKENKDSLTTLELEQLLGYKPEDVFEGNTKTSDLAIVWSMMRFVNTEQGKNKVVGVSLKNSNGTSEEIIYGHKAWKMLLGLNYDEMNYYDNSLLYWANELLTNGLTSKIIQWPVKMTRKYIDYNLSDITFNRNKKMNYWSNKDLFSTQFVSYQEDSKQEVLNYKIMYKVKKDVYYTYNVSVYKENTNSKYKINSFNRI"
                     /label="Mfl157"
                     /note=""
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAACGATGAT GCTCACTCTC GGGTCTCAAA TGAAGAAGAT
     2041 TCTGCTGTCT TTGAGCGCGC TGACGATTGG CGCCACCCCT GCAGTCTCCC TGCTGAATGT
     2101 TGGTCAGAAA CAGGTCATCA CCCAGGTGAC CAGCGAGATC CAGAAGAAGA TTGATGAAAC
     2161 CAGCGTCCTG TTCAAAGCCC CGATCATTAC CAACGCCCAA AAGCAGAACA GCGACGTTAC
     2221 CACGAACTGG CTGAATGATC AGAAAGTGTC GGATATTAGC AATATCAATA GCGCGTTGAG
     2281 CTACGCCAAT TTCAACGCAG AAAAATATGA CATGAAAAGC GTAGAAAAGA CGCTGAACAA
     2341 AATTTATAAA GACAAAGACT ATAAGTATAA AAACGAGGAC GCGACGTCTG AACTGATTTT
     2401 CGGTGCTGCA GATAAAGGTG TCAGCTTGCT GGAGAACAGC ATCGATGATG ATGGTTTGAA
     2461 AGCTGAGGGC GTTAACGAAT TGTTGAACTG GATCAGCTAC GTGTCCTTTG GTACTGGTCT
     2521 GGTGGGTGCG GTTAATGCCA AATCCATCGA TAAAAGCATT CTGAACCTGA ATAAGAATAT
     2581 TGAGTGGATC GCTGCCTTTA AAAATACTCC GATTCCGACG CTGAGCAAAC TGAACAACGA
     2641 GTACGACGTG TATACGTTCC AGGACTTGCA GGACTTTATT ATTATTTCGA TTGGTAACCT
     2701 GTTGAGCGAA GCCAGCGGTT TTGAGGGTGA TTCCTTCGCA CAAAGCACGG GTTGGGGCGG
     2761 TCGTACGAAA TGGAACAACA TTGTGAAAAA GGAATTAGAT CTGGATACTA TGAAAAAGGT
     2821 TGCGATCCCG CAGAAGAATA AGAATAGCAG CAACGTTCCG AAAGGCCAGA CCTCTTGGGA
     2881 CACCACCACC AACGGCAATA ATCGCGAATT CAGCATCTAT GACGAACGTT CCATCGTGCA
     2941 GCAAGAAACG GACTTTATCC AGTCCATTGT CAAACACATT ATTAACAGCG GTAACGGCTT
     3001 GAAAATCGAA ATGAATAGCA AGAATCTGCA ATCCATTGTG AACATCGTTT ATTCGATCAG
     3061 CTGGTACATC AGCTCCTATG ACAAGTACAG CGTGTATGTG ATGCCGTCTG ATGACAATTA
     3121 CCTGTTCAGC GAAACCAAAA GCAATATGAC GATCTTTAAC GAACAACGTA CTAAAACCAT
     3181 TGAGAAAACC GATTTAAGCA ACAGCTTGTC CGGTATCCTG AGCCTGTTCA AAAGCGTGTT
     3241 CATTAGCAAG GACCGCATGA CTAGCAATCG TGGTCTTAAG ATCCTGTTTC AGAACGGCGA
     3301 TAAGACTACG AACAACTGGG GTTCCGAACT GAGCATCTTC CTGAGCTTTA TTGGTGGTAC
     3361 CTGGACCCGC AACAACTTTA ACAATGGTCT GAATGCATTG CTGATTGGTG CCATGGCCGG
     3421 TGTTACGAAC AGCAAACTGT TGGACAACGT TAAAAGCATT GATACGATTC TGACGCTGGT
     3481 GGGCCTGAAC CGTAATTCCT TAGGTTACGT GATTGGCAAC ATTCTGAACG CGACTCTCGC
     3541 TAACACCAAT AGCACGAACG TCTTTTATGG TCTGGCAACG AGCACGCTGG TGACCATTGT
     3601 GGGTGGTATC ATCCCGGATG TCGGTAAATA TCTGGACGAG ATCAAGAAAA TGGCGAATGA
     3661 TAATGCGTTC GACCACACTA TGACCAAACT GGTTAGCACG AAGCTGAACG TGCTGATGGA
     3721 CTACTTGGGT AATGTTATGG ACGTGTTCAG CCCGCCGGCA GAGACTGACC CTCCGCGTGA
     3781 GCCGTCTACC CTGCCGAATA AGAGCATTCT GGAGTTCATG AATATGGGCG TTATGGGTAT
     3841 TACCGTCGGT AACGTTATCT GGAGCGGCCT GAACTTTATT AACACGAGCC TGAGCGAAAT
     3901 CATGAAGCCG CTGGGCCTGT TGGGTGCGCT GCTGAGCGAC GATGTGTCCC GTAACTATAA
     3961 CTTTTACGTT AAGGATAAGG AAAACAAGGA CAGCTTGACG ACGCTGGAGC TGGAACAATT
     4021 ACTGGGCTAC AAGCCGGAGG ACGTGTTTGA GGGTAACACG AAAACGAGCG ACCTGGCCAT
     4081 CGTCTGGAGC ATGATGCGTT TTGTAAATAC GGAACAAGGC AAGAACAAAG TTGTCGGTGT
     4141 TTCTCTGAAG AATAGCAACG GTACGAGCGA GGAAATCATC TATGGCCATA AGGCGTGGAA
     4201 GATGCTGCTG GGCTTGAATT ATGATGAAAT GAACTACTAT GACAATAGCT TGCTGTACTG
     4261 GGCAAACGAG CTGCTGACGA ACGGCCTGAC CAGCAAGATT ATTCAGTGGC CGGTGAAAAT
     4321 GACGCGCAAA TACATCGACT ACAATCTGAG CGACATTACC TTTAACCGTA ATAAGAAAAT
     4381 GAATTACTGG TCTAATAAGG ACCTGTTTAG CACCCAGTTC GTTAGCTATC AAGAGGACTC
     4441 CAAGCAAGAA GTCCTGAACT ACAAGATTAT GTATAAAGTG AAGAAGGATG TTTACTATAC
     4501 GTATAATGTC AGCGTATACA AAGAAAATAC CAACAGCAAA TATAAGATTA ACAGCTTCAA
     4561 CCGCATTTGA AGAGCTTAGA GACCCGCATG TAGGCACCAT CTAT
//