LOCUS       Exported     4553 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4553
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4519
                     /label="Mfl158"
                     /note=""
                     /translation="MKKLISLIGAASLATTPAMAVVSCKYIDTIQKSIDNKLAEVISSTSNYFKGAILANSEDYNGQSVDKYISKLKVGDLTSNSKDTTSMAKIFDTFLDTKQSSEYMKNEVYSKEEFDRPATGNGASELISTIQKAYNEIYKLIGVNFDPTLWNTALPLLLDSLKADNISSIQKVMPKISSSLQIMKDVKVTNYNELIKLGVETNLDLKIYFSNELTSFVANLFSVEIEKPFVISENKDNINEKYYAHNSSVWKLIIKKMNSKDAEKPEINFTSQSLALLMNALFGINWYIQNFTADEYNLHSDEMLDDNHIFSTDKTNLEVIHQVNSSKIDKEIINSTNVDGLMQFIYDLFSGEKDKESFKLLRTFKILFQVDDDITVKNQKLDFNTKIIKFGSKTLDFKIGEWGSNGKTENGALNTFLSSSLDGLVKGYTASFDPDGGIMDFLNSIGMGSEQIGGVVSGLISSILTGIDMDHFFETFIKQIDDSLKNLLEWSFIKNKPLAEQITSIQTSVNTKVKPIIDSLLNSEAPFTNKFLGYLINTDIRKLMEIFGLENSLPVQLPKISLKQIIDIQLTKTLKLSDLLRLGAKGTSYLIALLGKGVAPKIVNIADAFNDTSLFLDPEFKIVNSNSTLQTLGEIKISPELIFKNEEGKEYTYILALATALSSRDNTGISITIPGGNKFGLKTTRTFTNKDGLKWIMGLGVELDNGVIDYNQFRPGTILYSIGTLWNDETGNLITGIMSGINDILKTIADIFDNKQNQSYLEDLNYIHYKTKLISYENFRDSKKDSHVTYEINYKNGIVNNTYVVKLLLPSSDQEEINVKYQIEAFYKK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGCCCAG AAGGATACAA CGGTCTCAAA TGAAAAAACT
     2041 CATCAGCTTG ATCGGTGCGG CTAGCCTGGC GACCACCCCT GCAATGGCTG TTGTTTCCTG
     2101 TAAATACATC GACACCATCC AGAAATCGAT CGATAATAAA CTTGCCGAAG TGATTAGCAG
     2161 CACCAGCAAT TATTTCAAGG GTGCCATCCT GGCGAATAGC GAAGATTACA ATGGCCAAAG
     2221 CGTGGACAAG TACATTAGCA AGTTGAAGGT GGGTGACTTG ACCAGCAATT CTAAAGACAC
     2281 CACCTCCATG GCAAAGATCT TCGATACTTT CCTGGATACG AAACAGAGCA GCGAGTACAT
     2341 GAAGAACGAG GTTTATAGCA AAGAGGAATT CGACCGTCCG GCAACCGGTA ACGGTGCTAG
     2401 CGAATTGATC AGCACCATTC AGAAAGCTTA TAATGAAATC TACAAGCTGA TCGGTGTCAA
     2461 CTTCGACCCT ACCCTGTGGA ACACCGCGCT GCCGCTGCTC CTGGATAGCC TCAAGGCCGA
     2521 CAACATTAGC AGCATTCAGA AGGTGATGCC GAAGATCAGC AGCAGCCTGC AAATTATGAA
     2581 AGATGTCAAG GTTACCAATT ATAACGAGCT GATCAAGCTG GGTGTGGAAA CGAATCTGGA
     2641 TCTGAAGATC TACTTTAGCA ATGAACTGAC CTCGTTCGTT GCGAACCTGT TCAGCGTTGA
     2701 GATTGAAAAA CCGTTCGTCA TCAGCGAGAA CAAGGACAAT ATCAATGAGA AGTATTACGC
     2761 CCATAACAGC AGCGTGTGGA AACTGATCAT CAAGAAGATG AACAGCAAAG ATGCGGAAAA
     2821 ACCGGAGATC AATTTTACCT CACAAAGCCT GGCGTTGCTC ATGAATGCGC TGTTCGGTAT
     2881 CAATTGGTAC ATTCAAAACT TTACCGCAGA TGAGTACAAT CTGCACTCCG ACGAGATGCT
     2941 GGATGATAAC CACATCTTTA GCACCGACAA GACCAATTTG GAGGTTATCC ATCAAGTTAA
     3001 TAGCAGCAAA ATCGACAAAG AGATCATTAA TAGCACCAAC GTCGATGGTC TGATGCAATT
     3061 CATTTATGAC CTGTTTAGCG GTGAGAAAGA CAAGGAGAGC TTTAAACTGC TGCGCACGTT
     3121 TAAAATTCTG TTCCAGGTAG ACGATGACAT CACTGTTAAA AATCAAAAGC TGGACTTCAA
     3181 CACCAAGATT ATCAAATTTG GCAGCAAGAC CCTGGACTTC AAGATCGGTG AATGGGGTAG
     3241 CAACGGCAAA ACCGAGAATG GTGCGCTGAA CACGTTTCTG AGCAGCAGCC TGGACGGCTT
     3301 GGTTAAGGGT TACACCGCAA GCTTCGATCC GGATGGTGGT ATCATGGATT TCCTGAACAG
     3361 CATTGGTATG GGCTCCGAGC AGATCGGCGG CGTGGTTAGC GGTCTGATCA GCAGCATTCT
     3421 CACGGGTATC GATATGGATC ACTTCTTTGA AACCTTCATC AAACAGATCG ACGACAGCCT
     3481 GAAAAATCTG CTGGAATGGA GCTTCATCAA GAATAAACCG CTGGCAGAAC AGATTACCAG
     3541 CATTCAAACC TCTGTTAATA CCAAAGTGAA ACCGATCATC GACAGCTTGC TGAATTCTGA
     3601 AGCGCCGTTC ACCAATAAGT TTCTGGGCTA CCTGATTAAT ACCGACATCC GCAAACTGAT
     3661 GGAAATCTTC GGTCTGGAGA ACTCCCTGCC GGTGCAACTG CCGAAAATTA GCCTGAAACA
     3721 AATCATTGAT ATTCAGCTGA CCAAAACCCT GAAACTGAGC GATCTGCTGC GTCTGGGTGC
     3781 CAAAGGCACC TCTTATTTGA TCGCGCTGCT GGGCAAAGGC GTTGCGCCGA AAATTGTCAA
     3841 TATCGCCGAC GCGTTTAATG ACACGAGCCT GTTCCTGGAT CCGGAGTTCA AAATTGTGAA
     3901 TAGCAACAGC ACCCTGCAAA CGCTGGGCGA GATTAAGATT AGCCCGGAAT TGATCTTTAA
     3961 GAACGAAGAA GGCAAAGAGT ATACCTATAT CCTGGCGCTG GCAACCGCAT TGTCCTCCCG
     4021 TGATAATACG GGTATTAGCA TCACGATCCC GGGTGGCAAT AAGTTTGGTC TGAAAACTAC
     4081 CAGAACCTTC ACCAACAAAG ACGGTCTGAA ATGGATTATG GGCTTGGGTG TTGAGCTGGA
     4141 CAATGGTGTT ATCGACTACA ATCAGTTCCG TCCAGGTACC ATCCTGTACA GCATTGGTAC
     4201 CCTGTGGAAC GACGAAACGG GCAATCTGAT CACGGGCATC ATGAGCGGTA TTAATGACAT
     4261 TCTGAAAACG ATTGCTGACA TCTTCGATAA TAAGCAGAAT CAGAGCTACC TGGAGGACCT
     4321 GAATTACATT CATTATAAGA CTAAACTGAT CTCTTACGAG AATTTCCGCG ATAGCAAAAA
     4381 AGACAGCCAC GTTACGTATG AGATCAACTA TAAAAATGGC ATCGTGAACA ACACCTACGT
     4441 GGTCAAATTG CTCTTGCCGT CGAGCGACCA AGAGGAGATC AACGTCAAAT ACCAGATTGA
     4501 GGCGTTTTAC AAAAAGTGAA GAGCTTAGAG ACCGGACCTT GACGCCTAAT ACT
//