LOCUS       Exported     4064 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4064
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2030..4030
                     /label="Mfl160"
                     /note="DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA."
                     /translation="MTKEQASKLINDLRLKLNEWAKEYYVLDNPSVDDAEYDKTIHQLLDLENQFPELITSDSITQKVGGIVSDKFEKHTHKYPMLSLGDIFSWDEFINFNKQVAKVTGTENNEYTAELKIDGLSISLIYKDGVLQKGVTRGDGKVGENVTTNVKTIKSIPLSIPSKDEIEIRGEVFLGKKEFAKINEERLLNGDQLFANPRNAAAGTLRQLDSKIVAERNLDAYLYYYFNENNPINTQFDSINQIKNLGLKINKETKICKTLEEVKLYIEYYTEKRNELDYEIDGIVFKLNDKKLQEEVGYTAKTPKWAIAYKFPAEVKQTKLLDIFPTVGRTGKITYNAKLEPVQIAGTTVSAASLNNAEYIMAKDLRINSIVKVKKAGDIIPEVISAIKDEKFDLLPIWEKDVQCPACNELLEKTSTEVDQFCVNFNCPAQILRSLEHFASRGAANIVGLGGQTIKKLFEEKLITNIADIFKVEEHKENIINFEKFGEKSFENLIASIKESKNNSFEKTLFGLGIRHVGSKTALTLAQIYKNIDNLKNATYEELSSIDSVGEVLAMSIVDWFKIESNLQLINELKSFDVNFEYLGQAKNTESTISEKSFVITGTLSESRDYFKDIIELNNGKVIGSVSKKTDYVLAGENAGSKLTKAEELNVKVINEEEFFAILKGE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAATAAGAC GACGACCCTC GGGTCTCAAA TGACGAAAGA
     2041 GCAGGCATCG AAGCTGATTA ATGATCTGCG TCTGAAACTG AACGAATGGG CGAAAGAATA
     2101 CTACGTTTTG GATAATCCTT CGGTCGACGA TGCCGAATAT GATAAGACTA TCCATCAATT
     2161 GCTGGATCTG GAGAACCAGT TCCCGGAGCT GATTACCAGC GATAGCATTA CTCAGAAAGT
     2221 TGGTGGCATT GTTAGCGACA AGTTCGAGAA GCATACCCAT AAATACCCGA TGCTGAGCCT
     2281 GGGCGATATT TTTAGCTGGG ATGAGTTCAT CAATTTCAAT AAACAGGTCG CCAAGGTCAC
     2341 GGGCACCGAA AATAATGAGT ATACCGCCGA GCTGAAGATT GATGGCCTTT CTATTTCCCT
     2401 GATCTATAAG GACGGTGTCC TGCAAAAGGG CGTTACCCGT GGTGATGGTA AGGTGGGTGA
     2461 AAACGTTACC ACCAACGTAA AAACTATCAA AAGCATTCCG TTGAGCATCC CATCCAAGGA
     2521 CGAGATCGAG ATTCGTGGTG AAGTTTTCTT GGGTAAAAAG GAATTTGCTA AAATCAACGA
     2581 GGAGCGTCTG CTGAACGGCG ATCAACTGTT CGCCAACCCA CGTAACGCGG CGGCCGGCAC
     2641 CCTGCGCCAG CTGGATAGCA AGATCGTTGC AGAACGTAAC TTGGATGCGT ATCTGTACTA
     2701 CTACTTCAAC GAAAACAATC CTATTAACAC CCAGTTCGAC TCTATTAATC AGATTAAAAA
     2761 CCTGGGTTTA AAAATTAACA AGGAAACCAA AATCTGTAAA ACCCTGGAAG AGGTTAAGTT
     2821 GTACATCGAG TACTATACCG AAAAGCGCAA TGAGCTGGAC TATGAGATCG ATGGCATCGT
     2881 TTTTAAGCTG AATGATAAAA AACTGCAAGA GGAAGTCGGT TATACCGCCA AAACGCCGAA
     2941 ATGGGCAATT GCGTACAAGT TTCCGGCAGA GGTTAAACAG ACGAAACTGC TGGATATCTT
     3001 TCCGACCGTG GGTCGTACCG GCAAAATCAC GTACAACGCA AAATTGGAAC CGGTGCAAAT
     3061 TGCCGGTACG ACGGTTAGCG CAGCTAGCCT GAACAATGCG GAGTACATTA TGGCCAAGGA
     3121 TCTGCGTATC AATTCGATCG TGAAGGTCAA GAAGGCGGGT GATATTATCC CGGAAGTTAT
     3181 TAGCGCCATC AAGGATGAAA AATTTGACCT TCTTCCGATT TGGGAGAAGG ATGTCCAGTG
     3241 CCCGGCGTGC AACGAACTGT TAGAAAAGAC TAGCACGGAG GTTGATCAGT TTTGCGTTAA
     3301 CTTCAACTGT CCGGCGCAGA TTCTCCGTTC GCTGGAGCAC TTTGCCAGCC GTGGTGCAGC
     3361 CAATATCGTT GGTCTGGGCG GTCAGACCAT TAAGAAACTG TTCGAGGAGA AGCTGATTAC
     3421 CAATATTGCG GACATCTTTA AAGTCGAAGA ACATAAAGAG AACATTATTA ACTTCGAAAA
     3481 GTTTGGTGAA AAGAGCTTCG AAAATCTGAT CGCCAGCATC AAAGAAAGCA AAAACAATAG
     3541 CTTTGAAAAG ACTCTGTTCG GCCTGGGTAT TCGCCATGTT GGCAGCAAAA CGGCACTGAC
     3601 GCTGGCGCAA ATCTATAAAA ATATCGACAA TCTGAAGAAT GCCACCTATG AAGAACTGAG
     3661 CAGCATTGAC AGCGTGGGCG AAGTTCTGGC AATGTCCATC GTCGATTGGT TCAAGATTGA
     3721 GAGCAATCTG CAACTGATTA ATGAATTAAA GAGCTTCGAT GTGAATTTCG AGTATCTGGG
     3781 CCAGGCGAAA AACACCGAAA GCACCATTAG CGAAAAAAGC TTCGTTATCA CCGGTACCCT
     3841 GAGCGAAAGC CGTGACTACT TCAAAGATAT CATTGAGCTG AATAACGGTA AGGTTATCGG
     3901 TAGCGTTTCT AAGAAAACCG ATTACGTTCT GGCTGGCGAA AATGCGGGTA GCAAACTGAC
     3961 CAAGGCCGAG GAGCTGAATG TAAAGGTCAT CAATGAGGAA GAGTTTTTTG CCATCCTGAA
     4021 GGGTGAGTGA AGAGCTTAGA GACCCGCGGA AAATGGAAAA CACT
//