LOCUS Exported 3758 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3758 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3724 /label="Mfl166" /note="" /translation="MNGGNQMSTNTNKSNKSKWLHFKKNKEQKNKFDPHKAFLKLKTWWPLSKVSGRIFLIYLFIVLFGGFLLCIPGIVVNNDLNGYDFRWDYLTGIFTASSAFSDTGINIIDPSHDYTFWGQLILLILIEMGGIGVLTLKIILFISINKKISLSDTVVAQSERGNDVKSSTIELIKDGFIFLTFIQLIAAGVLFFLFFFSEPATQSLNGTELNVVSPYHNFIKSIWFAIFHSTSAINNAGYDLLSTNSLQPYNIEGHQAYAIQIVFLLEWVIGGLGYPTFHDIKRKMRARRVGQKVKFSLFTKLNFWVYSTLFVVGPLLIFLSEYSNQTNSLIFNYYTYDVDPLTQMPINVIVTEAKPTFVVAMDIIFNTTACRNAGFSTVPINDFNASSKTILSSLMFIGSAPSSTAGGIRTTTFAIILLSTWAIIRNKSYTSAFKKVIPAETVRRSFSVFFISVFILTIVIILIYFDSNGFLTPGIENGVAGELVQNQGDASIVQILTLITSAYGTVGMNPFTQHQMYNFGVLTKLLIILCMFLGQLGISNTLLAFIKPSRKTNFKYLEEDVTIG" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAGGATATG TAGACACCGC CGGTCTCAAA TGAACGGTGG 2041 CAATCAAATG AGCACGAATA CCAATAAAAG CAATAAGAGC AAGTGGCTGC ACTTCAAAAA 2101 GAATAAGGAG CAGAAAAACA AATTTGATCC GCACAAGGCC TTTCTGAAAC TCAAGACGTG 2161 GTGGCCGCTG AGCAAAGTCA GCGGCCGCAT TTTCCTGATC TATCTCTTTA TCGTACTTTT 2221 CGGTGGTTTC CTGCTGTGCA TCCCGGGCAT CGTTGTCAAC AATGACTTGA ATGGCTATGA 2281 CTTCCGTTGG GACTACCTGA CTGGTATCTT TACCGCGTCC TCTGCCTTCA GCGACACCGG 2341 CATTAACATC ATCGATCCTT CTCACGACTA CACCTTTTGG GGTCAGCTGA TCCTGCTGAT 2401 CCTGATCGAA ATGGGTGGTA TCGGTGTGCT GACCCTGAAG ATCATTCTGT TCATCAGCAT 2461 CAATAAGAAA ATCTCCCTGA GCGACACGGT CGTGGCTCAG TCCGAGCGTG GTAACGATGT 2521 CAAGAGCAGC ACGATCGAAC TGATCAAAGA CGGTTTTATT TTTCTGACTT TCATCCAACT 2581 CATTGCCGCT GGTGTTCTGT TTTTCCTGTT CTTCTTTAGC GAGCCTGCGA CTCAATCCCT 2641 TAATGGCACG GAATTGAATG TGGTGAGCCC GTATCATAAC TTCATTAAAA GCATCTGGTT 2701 TGCGATTTTC CATAGCACGA GCGCGATCAA CAATGCCGGT TACGATCTGT TGTCTACCAA 2761 CAGCCTGCAA CCGTACAACA TTGAGGGTCA CCAGGCGTAT GCCATTCAGA TTGTGTTTTT 2821 GCTGGAGTGG GTTATCGGCG GTCTGGGTTA TCCGACGTTT CATGATATTA AACGTAAAAT 2881 GCGCGCCCGT CGCGTCGGTC AGAAGGTTAA GTTCAGCCTG TTTACTAAGC TGAATTTCTG 2941 GGTGTACAGC ACCCTGTTTG TTGTCGGTCC TCTGCTGATC TTCTTGAGCG AATACAGCAA 3001 CCAGACCAAT AGCCTGATTT TCAATTACTA CACCTATGAT GTCGATCCGC TGACCCAAAT 3061 GCCGATTAAC GTTATCGTGA CCGAGGCGAA ACCGACGTTC GTTGTGGCCA TGGATATCAT 3121 CTTTAATACT ACCGCGTGTC GTAACGCGGG TTTTTCTACG GTTCCGATTA ATGACTTTAA 3181 TGCCAGCAGC AAGACCATTC TGAGCAGCCT GATGTTTATT GGTAGCGCAC CGTCCAGCAC 3241 GGCGGGTGGC ATTCGTACCA CCACCTTCGC GATTATCCTG CTGAGCACCT GGGCAATCAT 3301 CCGTAATAAG TCCTATACCA GCGCATTCAA AAAAGTTATC CCTGCAGAGA CTGTCAGACG 3361 TAGCTTCTCC GTGTTCTTTA TTTCCGTTTT CATCCTGACC ATCGTGATTA TTCTGATCTA 3421 TTTTGATAGC AACGGCTTTT TGACCCCGGG CATCGAGAAC GGTGTGGCCG GCGAACTGGT 3481 CCAGAATCAA GGTGATGCCA GCATTGTTCA GATCCTGACC CTGATTACCA GCGCCTACGG 3541 CACCGTCGGT ATGAATCCGT TCACTCAACA CCAAATGTAC AACTTCGGTG TTTTGACCAA 3601 GCTGTTGATC ATCTTATGTA TGTTCCTGGG CCAACTGGGC ATCAGCAACA CCCTGCTCGC 3661 GTTTATTAAA CCGTCGCGTA AAACGAACTT CAAATACCTG GAGGAGGACG TTACCATTGG 3721 CTGAAGAGCT TAGAGACCGT ACGTCTGAAC TTGGGACT //