LOCUS       Exported     2998 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2998
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1036..1083
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2931..2947
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2948..2979
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..964
                     /label="Mfl180"
                     /note=""
                     /translation="MIYTVTLNPALDHIIETDGFNIGETNYYKNEYVVIGGKGINVSIILNNLEAKVLSTGILGSNNKNSFLEKFDENNLKNKFFINKGVTRTNLKIKNLSKFEETELNGLGSTVSLEIINELKTFLNDNLKSGDILVAAGSIPAGVKNNIYEEIGNIANEKNALFILDTSKVNMLNGLKTKPYLIKPNIEEICEILNLPFKEYTFEETCEMVKKLKSLGARNVLLSRGSKGSYFFSEKGEIFETGIAKGKLVNSVGSGDSMIAGFTYGLYKNLSIEECLQFGAAAGGATAFTEWLGLKEDILKLKQEIKVNKIK"
     primer_bind     complement(1088..1104)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1171..1759)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1950..2810)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2811..2915)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GATTTACACC GTGACGCTGA ATCCGGCGCT
       61 GGATCACATC ATCGAAACGG ACGGCTTTAA CATCGGTGAA ACCAACTACT ACAAGAACGA
      121 ATACGTTGTT ATCGGTGGCA AAGGCATCAA CGTAAGCATC ATCCTGAATA ATCTGGAAGC
      181 AAAAGTTCTG AGCACCGGTA TCCTGGGCAG CAATAATAAA AACAGCTTTT TGGAGAAGTT
      241 TGACGAGAAC AATCTGAAAA ATAAGTTCTT CATCAATAAA GGCGTCACGC GCACTAATCT
      301 GAAGATTAAA AATCTGAGCA AATTCGAAGA AACCGAGCTG AACGGTTTAG GTTCTACGGT
      361 TTCCCTGGAG ATTATCAACG AACTTAAGAC CTTCCTGAAC GACAACCTTA AGAGCGGTGA
      421 TATCCTGGTG GCAGCGGGTT CCATCCCGGC GGGTGTTAAA AATAACATCT ATGAGGAGAT
      481 TGGTAACATT GCAAATGAAA AGAACGCGCT GTTCATTCTC GATACGAGCA AGGTTAATAT
      541 GCTGAACGGC CTCAAGACCA AACCGTACCT GATCAAGCCG AATATCGAGG AAATCTGCGA
      601 GATCCTGAAT CTGCCGTTTA AGGAGTACAC GTTTGAGGAA ACGTGCGAGA TGGTCAAAAA
      661 ACTGAAATCC CTGGGCGCGC GTAACGTTTT GTTGAGCCGC GGCTCTAAGG GTAGCTATTT
      721 CTTCAGCGAA AAAGGTGAGA TCTTCGAGAC TGGTATCGCA AAAGGTAAAC TGGTGAACAG
      781 CGTCGGTTCC GGCGATAGCA TGATTGCCGG TTTTACCTAC GGTTTGTATA AGAATTTGAG
      841 CATTGAAGAA TGCCTCCAAT TCGGCGCGGC CGCGGGTGGT GCGACCGCAT TCACCGAGTG
      901 GCTGGGTCTG AAGGAGGATA TTCTGAAACT GAAACAGGAG ATCAAAGTCA ATAAAATCAA
      961 GTGAAGAGCT TAGAGACCGG CTCGATACAG TTTCACGTCG CTGAGGTGTC AATCGTCGGA
     1021 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1081 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1141 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1201 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1261 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1321 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1381 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1441 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1501 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1561 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1621 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1681 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1741 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     1801 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     1861 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     1921 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     1981 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     2041 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2101 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2161 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2221 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2281 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2341 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2401 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2461 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2521 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     2581 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     2641 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     2701 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     2761 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     2821 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     2881 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     2941 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG
//