LOCUS       Exported     4211 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4211
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4177
                     /note=""
                     /translation="MELKDLFKSKVSVFKADLKTKEEVINFLVDKLSSEKMIANKKTFKDAILKRESEASTGMGDGIGIPHAINNTVKEPCIAFVSLKSPIDWQSLDNKPVDLIFMIATNDEKGEAHLGALADLSKFLMKPEFQEALRKAKTFKDLSKAFDNKVETKKVEAKNGKYDVIGITACPTGIAHTYLAEEKLIEYATELGLSVKIETQGRRGTENKLTQEDVDNAKVIILAHDKNLQGMGRFGGKQVIDTTTKDAIFNGKQLIQEFGKTEKTTTAKAVSSKDDEVSDDFSLKKFAQVKGNLLAGVSRMLPFVVAGGIILGIGFLIDFAAGNGNVPGMTGATFDYWFNNIYDKTKVDVLTEWTNWWMGNFGTHSEVAGWFSAIGKTGMMMMVPVLAAYISYTIVGPQGLMPGFIAGILADGTGGFAYAGDKFGWSGLWTRLIPADIPMQSGFIGGMVGAYVAALIVFGLTIGFKKFKKSFHGVRDIVLIPVLSLLGISLAMFALNIPLGYTMYGLQQFLKLLADQNLLILLGAILGLMMCIDMGGPINKIAYVTGTLSVSGGLGNDPLITVTMAAAMAGGMIPPLGIALCTVTFRKAWTTKEKDSAKANWLMGAFFISEGAIPFMVTDPKRISVSAMAGGTITGLIVGGCKITLGAPHGGIAVFPLLKSGLLGTSPEQMNSGAAIGLGVGLYILAIVVGTLVMATILGFWKTYDIKKGKLVIAN"
                     /label="Mfl181"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCAAA TGGAGCTGAA
     2041 GGACCTGTTT AAAAGCAAAG TTAGCGTTTT TAAAGCGGAC CTGAAAACCA AGGAGGAGGT
     2101 GATTAACTTC CTGGTGGACA AGCTCTCTTC TGAGAAAATG ATTGCCAACA AGAAAACCTT
     2161 TAAAGATGCG ATCCTGAAAC GTGAAAGCGA AGCCAGCACG GGTATGGGTG ATGGCATCGG
     2221 TATTCCGCAC GCAATTAATA ACACCGTGAA GGAGCCTTGC ATTGCATTTG TGAGCCTGAA
     2281 AAGCCCGATT GACTGGCAAT CGTTGGATAA TAAGCCTGTG GATTTGATCT TCATGATCGC
     2341 GACCAACGAC GAAAAGGGTG AGGCTCACCT GGGTGCGCTG GCGGACCTGA GCAAATTTCT
     2401 GATGAAGCCT GAATTCCAAG AGGCCTTGCG TAAAGCTAAG ACCTTTAAAG ATCTGAGCAA
     2461 AGCGTTCGAT AACAAGGTGG AAACCAAGAA AGTTGAGGCA AAAAACGGTA AGTATGACGT
     2521 GATTGGTATC ACGGCGTGTC CGACCGGTAT CGCGCACACT TACCTGGCTG AAGAAAAACT
     2581 GATTGAATAC GCGACCGAAC TGGGTTTGTC CGTGAAAATT GAAACGCAAG GTCGTCGCGG
     2641 TACGGAGAAT AAACTGACCC AGGAGGACGT TGACAACGCG AAGGTGATTA TCCTTGCCCA
     2701 TGACAAGAAT CTGCAAGGCA TGGGTCGTTT CGGTGGTAAA CAGGTCATTG ATACCACGAC
     2761 CAAGGATGCA ATTTTCAACG GTAAGCAATT AATCCAGGAA TTTGGTAAAA CGGAGAAAAC
     2821 CACCACCGCG AAGGCGGTTA GCAGCAAGGA CGATGAGGTA AGCGACGACT TTAGCCTGAA
     2881 AAAGTTCGCA CAGGTCAAAG GCAATCTGCT GGCAGGCGTT AGCCGCATGT TGCCGTTTGT
     2941 AGTTGCGGGT GGCATTATTT TGGGCATTGG TTTTCTGATT GACTTCGCCG CCGGTAATGG
     3001 CAACGTTCCA GGTATGACCG GTGCGACGTT CGATTACTGG TTTAATAATA TCTATGATAA
     3061 AACTAAGGTT GACGTCCTGA CGGAATGGAC CAACTGGTGG ATGGGTAACT TCGGTACCCA
     3121 CAGCGAGGTG GCGGGTTGGT TCAGCGCGAT CGGCAAAACG GGTATGATGA TGATGGTTCC
     3181 GGTCCTGGCG GCGTATATTA GCTACACCAT CGTCGGTCCG CAAGGCCTGA TGCCGGGTTT
     3241 TATCGCGGGT ATTCTGGCGG ACGGTACCGG TGGTTTTGCT TATGCTGGTG ATAAATTTGG
     3301 TTGGTCTGGC CTCTGGACGC GCCTGATCCC GGCAGACATC CCGATGCAGA GCGGTTTTAT
     3361 CGGCGGTATG GTGGGTGCGT ATGTGGCGGC ACTGATCGTT TTTGGCCTGA CGATCGGCTT
     3421 TAAGAAATTC AAGAAGTCCT TTCACGGCGT TCGTGACATT GTCTTGATCC CGGTCTTGAG
     3481 CCTGCTGGGT ATTAGCCTGG CAATGTTTGC ACTGAATATT CCGCTGGGCT ATACGATGTA
     3541 CGGCCTGCAA CAGTTCCTGA AACTGCTGGC CGACCAGAAC TTGCTGATTC TGCTGGGCGC
     3601 TATTCTGGGC CTGATGATGT GTATCGACAT GGGTGGCCCA ATCAACAAAA TCGCTTATGT
     3661 TACCGGTACC CTGAGCGTTA GCGGCGGCTT GGGCAACGAT CCGTTGATCA CGGTGACCAT
     3721 GGCGGCGGCT ATGGCCGGTG GTATGATCCC ACCGTTGGGT ATCGCACTGT GCACGGTGAC
     3781 CTTCCGTAAA GCGTGGACGA CCAAGGAGAA GGATAGCGCG AAGGCGAATT GGCTGATGGG
     3841 TGCGTTTTTT ATCAGCGAGG GTGCGATTCC GTTCATGGTT ACTGATCCGA AGCGCATTAG
     3901 CGTTAGCGCA ATGGCGGGCG GCACCATCAC CGGTTTGATT GTTGGTGGTT GCAAAATCAC
     3961 GTTGGGTGCG CCGCATGGTG GTATCGCGGT TTTCCCGCTG TTAAAGAGCG GTCTGCTGGG
     4021 TACGAGCCCG GAGCAAATGA ATAGCGGCGC GGCGATCGGC TTGGGCGTAG GTCTGTACAT
     4081 CCTGGCAATT GTCGTTGGCA CGCTGGTTAT GGCGACCATC TTGGGCTTTT GGAAAACCTA
     4141 CGACATTAAG AAGGGTAAGT TGGTTATTGC GAACTGAAGA GCTTAGAGAC CGGCTGCCAA
     4201 AGAAACTGAT T
//