LOCUS Exported 3358 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3358 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1396..1443 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3291..3307 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3308..3339 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1324 /label="Mfl182" /note="" /translation="MDLKTPIVELSNVSKIYNKQIWALKKIDLKIYRGECVSLLGSNGSGKTTLLRIIGNNLKNTTGTINYNLEEENILKGIGLQKREQAWPNGFKVKDINDLWIRIYDVNDLEWINKLKDVFGVNEVEEKYLNKLSIVKLQIYAIFLSFISKPELVLIDELSSDIDFKYEEKITNFFKEYLEEGNTLILNSPSYYFLENLTDRVVYLNDGEIFEDLSIKEVKKEYKSVMEYTKAIFKEELVVEKKIKNKSKFFSTMISKTEGYSNVLQAIIEQLEAQENCNEKVLSRLKEVYFSILDLNTSIDNLSVSYINADSIKIISKKIKLTMKLINKLTINYRYKKYHKTLSGIEKFLSKELKRTFANDKVIVNGDVLSITMSQSEKKQLEKLKEKYIKEEQKIIRRKILKQQFKKQKDMKSKNNPVQESLKEVTTDENK" primer_bind complement(1448..1464) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1531..2119) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2310..3170) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3171..3275) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 AAAGCACTCT TAGGCCTCTG GGTCTCAAAT GGACCTCAAG ACCCCGATCG TTGAACTGTC 61 GAACGTATCC AAAATCTACA ATAAGCAAAT TTGGGCTCTG AAAAAGATCG ACCTGAAGAT 121 TTACCGTGGT GAGTGCGTTA GCCTGCTGGG TAGCAACGGT AGCGGTAAAA CCACCCTTCT 181 GCGTATTATC GGCAACAACC TGAAGAATAC CACGGGTACG ATTAATTATA ATCTGGAGGA 241 GGAGAATATT CTGAAGGGCA TCGGTTTGCA GAAACGCGAG CAGGCGTGGC CGAACGGTTT 301 CAAGGTTAAA GATATCAACG ATCTGTGGAT CCGTATCTAT GACGTTAACG ATTTGGAATG 361 GATTAATAAG CTGAAGGACG TTTTCGGTGT GAACGAAGTT GAAGAGAAGT ATCTGAATAA 421 ACTTTCTATC GTTAAGTTGC AAATCTATGC AATCTTCTTG AGCTTCATCA GCAAACCGGA 481 GCTGGTTCTC ATCGACGAGC TGTCCAGCGA TATTGATTTC AAGTACGAGG AAAAAATCAC 541 CAATTTCTTC AAGGAATACC TGGAGGAGGG CAACACGCTG ATTCTGAACA GCCCGAGCTA 601 CTACTTCCTG GAGAACCTGA CGGACCGTGT TGTCTACCTG AACGACGGCG AGATCTTTGA 661 GGATCTGAGC ATCAAGGAGG TCAAGAAGGA ATACAAGAGC GTTATGGAAT ACACCAAAGC 721 GATTTTTAAG GAGGAGTTGG TTGTGGAGAA AAAGATCAAA AATAAATCTA AGTTCTTTTC 781 CACCATGATC AGCAAGACGG AAGGCTACAG CAACGTGCTC CAGGCGATCA TCGAACAGCT 841 GGAAGCGCAA GAGAACTGCA ACGAGAAGGT GCTGAGCCGT CTGAAAGAGG TGTATTTCTC 901 CATTCTGGAT CTCAACACCA GCATCGACAA CCTGTCTGTC TCCTACATTA ACGCCGACAG 961 CATCAAGATT ATTAGCAAAA AGATTAAGCT GACCATGAAA CTGATTAACA AGCTGACCAT 1021 CAACTACCGC TATAAAAAGT ATCACAAGAC CCTGAGCGGC ATTGAGAAGT TCCTGAGCAA 1081 AGAACTGAAA CGTACCTTCG CAAATGATAA AGTTATCGTG AACGGTGATG TGTTGAGCAT 1141 CACCATGAGC CAGAGCGAAA AGAAACAACT GGAGAAACTG AAAGAGAAAT ACATCAAGGA 1201 GGAGCAGAAG ATCATTCGCC GCAAAATTCT GAAACAGCAA TTTAAAAAAC AGAAAGACAT 1261 GAAAAGCAAA AACAATCCGG TCCAGGAATC TCTGAAGGAA GTTACCACCG ATGAAAACAA 1321 GTGAAGAGCT TAGAGACCGT GATATGCCGA CTCCTTGTCG CTGAGGTGTC AATCGTCGGA 1381 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT 1441 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT 1501 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA 1561 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 1621 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 1681 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 1741 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 1801 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 1861 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 1921 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 1981 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 2041 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 2101 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT 2161 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 2221 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 2281 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 2341 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 2401 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC 2461 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 2521 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 2581 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 2641 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 2701 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 2761 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 2821 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 2881 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 2941 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 3001 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 3061 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 3121 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 3181 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 3241 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC 3301 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG //