LOCUS Exported 3079 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3079 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1117..1164 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3012..3028 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3029..3060 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 29..1045 /label="Mfl183" /note="" /translation="MKTNKNFKIFLRLMKAQTFNYITDPINIILGFVLTTVTMLCWLAFKPHDAESGLLADSFVLASAIGISAIRNSQYNLNLTLADWRETRFIRNLLTTPVSKKVLYASILCFNWIVNILVTIILISLAMLFSSQRKVISNVQWTPFLIGFILNIFLSNVIALFLSTTFKNKEYVFIISLLSYFGPMYLLGLGIPWNVVGQIPAINILTYIAPHRYTLHLMQAAWVGNASNMSFPGVESSSWLGVHGFGYGGNGWWLPALIACIFIFIFAFAFYYRLKSNYQFGTRKYSKFKGVKKHANNIELIKRTNSIEELKILVEIAGLDYKTKKVKKKKPSKKRGDK" primer_bind complement(1169..1185) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1252..1840) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2031..2891) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2892..2996) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGACGATCC GAGCCATTAC GGTCTCAAAT GAAAACGAAC AAGAACTTTA AAATCTTCCT 61 GCGTTTGATG AAGGCCCAAA CCTTTAATTA TATTACCGAT CCGATCAACA TCATCCTGGG 121 CTTCGTTCTG ACGACCGTTA CGATGCTGTG TTGGCTCGCC TTTAAGCCGC ACGATGCGGA 181 ATCCGGTCTG CTGGCGGATA GCTTTGTGCT GGCGAGCGCT ATCGGCATTA GCGCAATCCG 241 TAATAGCCAA TACAATCTGA ACTTGACCCT GGCAGATTGG CGTGAAACCC GTTTTATTCG 301 CAATCTGCTG ACCACCCCGG TTAGCAAAAA GGTGCTGTAT GCTAGCATTC TGTGCTTCAA 361 CTGGATTGTT AATATTCTCG TTACTATTAT TCTGATCAGC CTGGCCATGT TATTCAGCAG 421 CCAACGCAAG GTCATTAGCA ATGTTCAGTG GACCCCGTTC CTGATTGGCT TCATCTTGAA 481 TATTTTTCTG AGCAACGTCA TTGCGCTGTT CCTGTCCACC ACTTTCAAAA ACAAAGAGTA 541 CGTTTTCATT ATTTCCCTGC TGAGCTATTT CGGTCCGATG TATCTGCTGG GCCTGGGCAT 601 CCCGTGGAAC GTGGTGGGTC AAATTCCTGC AATTAACATT CTGACCTATA TTGCCCCGCA 661 TCGTTACACG TTGCACCTGA TGCAGGCGGC GTGGGTGGGT AATGCGAGCA ACATGAGCTT 721 CCCGGGTGTT GAGTCGAGCT CCTGGTTGGG CGTCCACGGT TTTGGTTACG GCGGCAACGG 781 TTGGTGGCTG CCGGCGCTGA TCGCCTGTAT TTTCATCTTT ATCTTCGCGT TCGCATTCTA 841 CTATCGTTTG AAATCTAACT ACCAGTTTGG CACTCGTAAA TACAGCAAGT TCAAGGGCGT 901 TAAGAAACAT GCTAATAATA TTGAACTGAT CAAACGTACC AACAGCATTG AGGAGTTGAA 961 GATCCTGGTT GAGATTGCCG GCCTGGATTA TAAGACTAAG AAGGTTAAGA AGAAAAAGCC 1021 GAGCAAGAAG CGTGGTGACA AGTGAAGAGC TTAGAGACCC CGATAGTTCC AGCTAGCATC 1081 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT 1141 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG 1201 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG 1261 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 1321 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 1381 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 1441 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 1501 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 1561 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 1621 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 1681 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 1741 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 1801 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC 1861 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 1921 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 1981 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC 2041 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA 2101 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA 2161 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC 2221 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT 2281 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC 2341 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT 2401 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC 2461 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG 2521 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT 2581 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG 2641 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA 2701 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG 2761 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG 2821 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT 2881 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 2941 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC 3001 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT 3061 TGGTCAGGTG GAGTGGGAG //