LOCUS       Exported     3340 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3340
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1378..1425
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3273..3289
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3290..3321
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1306
                     /label="Mfl196"
                     /note="Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA."
                     /translation="MILVETNNSRKIAFEILKKVFKNKSFSNILLNDVSKMPISDKFKNLIFAIVHGTITNQILLDHVSRKLIDVKKTNIDIQILLWMSIYQLRFLKTIPQYAVVNESVMIAKSVNHKFSGLLNACLKKVIKNEEELFDFSFKSENQKICIENSFPKNLFNLIEKGYGKDIAKKVAIDSNQKPVISFRVNTLKISENDFFEKYKDEYELEKTEIKNCLISKKAIVKSDAYTSGQITIQDPASILVGNVLNPTKGSKVLDMCSAPGGKLTHLSMIMENTGQVTGFEISENKIKLIEENIKRLDCKNISLKCGDATLINEKENFDFILLDAPCSGFGVLKRKPEIKINNIEINNINNIVNIQEKLLDTAYYNLKQQGVMVYSTCTINPAENQNQIKKFIEKYKDIKIISENQLFGYEMNTDGFYICKMIKE"
     primer_bind     complement(1430..1446)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1513..2101)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2292..3152)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3153..3257)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCAAAGC TATTAGCGCG GGTCTCAAAT GATTCTTGTG GAAACGAATA ACAGCCGTAA
       61 GATTGCGTTC GAAATTTTGA AAAAAGTGTT TAAAAACAAG TCTTTCTCAA ACATCCTGCT
      121 GAACGATGTG TCCAAGATGC CGATCAGCGA CAAATTCAAA AACCTGATCT TTGCGATCGT
      181 TCATGGTACC ATTACCAACC AAATCTTGCT GGATCACGTT AGCCGCAAAC TGATTGATGT
      241 GAAAAAGACT AACATTGACA TTCAGATTCT GCTGTGGATG AGCATCTATC AGCTGCGTTT
      301 CTTGAAAACG ATTCCACAAT ATGCCGTTGT TAACGAGAGC GTCATGATCG CGAAATCCGT
      361 GAACCACAAA TTCAGCGGCC TTCTGAACGC GTGTCTGAAG AAAGTGATTA AAAACGAGGA
      421 GGAACTGTTC GACTTTAGCT TCAAGAGCGA AAACCAGAAG ATTTGCATTG AAAACAGCTT
      481 CCCGAAGAAT CTGTTTAACC TGATCGAGAA AGGTTATGGT AAGGATATCG CTAAAAAGGT
      541 TGCAATCGAC AGCAACCAAA AACCAGTCAT CAGCTTTCGT GTCAACACGC TGAAAATCAG
      601 CGAGAATGAT TTTTTCGAGA AGTATAAGGA CGAATACGAG CTGGAGAAAA CGGAGATTAA
      661 AAACTGTCTG ATCTCGAAGA AGGCCATCGT CAAGTCCGAT GCGTACACCA GCGGTCAGAT
      721 CACCATTCAA GATCCTGCAA GCATTCTGGT TGGCAACGTT CTGAACCCGA CGAAGGGCAG
      781 CAAGGTGCTG GACATGTGTA GCGCCCCTGG TGGTAAACTG ACTCATCTGA GCATGATTAT
      841 GGAGAATACG GGTCAAGTTA CTGGCTTTGA GATTAGCGAA AATAAAATTA AGCTGATCGA
      901 AGAAAATATC AAGCGCCTGG ATTGCAAAAA TATTAGCCTG AAGTGCGGTG ACGCGACGTT
      961 GATTAATGAG AAAGAAAACT TTGATTTTAT TCTGCTCGAT GCGCCGTGCA GCGGCTTTGG
     1021 TGTTCTGAAG CGCAAACCTG AGATCAAGAT TAATAATATC GAAATCAACA ATATCAACAA
     1081 TATCGTGAAC ATCCAGGAAA AGCTGCTGGA TACCGCCTAC TACAACCTGA AGCAGCAGGG
     1141 CGTTATGGTT TATTCTACGT GCACCATCAA TCCGGCAGAG AACCAAAATC AGATTAAGAA
     1201 GTTTATTGAG AAGTACAAGG ATATTAAAAT CATCTCGGAG AACCAACTGT TTGGCTATGA
     1261 GATGAACACC GACGGCTTCT ATATCTGCAA AATGATCAAG GAGTGAAGAG CTTAGAGACC
     1321 GCGTATACCT TGCTTCCTGT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     1381 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1441 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1501 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1561 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1621 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1681 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1741 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1801 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1861 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1921 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1981 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     2041 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     2101 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     2161 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     2221 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     2281 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     2341 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     2401 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     2461 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     2521 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     2581 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     2641 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     2701 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     2761 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     2821 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     2881 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     2941 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     3001 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     3061 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     3121 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     3181 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     3241 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     3301 TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGGGAG
//