LOCUS       Exported     3370 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3370
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1408..1455
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3303..3319
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3320..3351
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1336
                     /label="engA"
                     /note="GTPase that plays an essential role in the late steps of ribosome biogenesis."
                     /translation="MKKGIVAIVGRPNVGKSSLFNRIIREKKSIVEDTPGVTRDRIYGTAEWLTREFIVIDTGGITLEDQPFAKEIKVQAEIAMEEADVIVFLLNHQEGLSDEDKMIAKILYKTKKPIVLAVNKYDKKTSDFDQYEYMSLGFGEPILISATHGIGTGDLLDDIIHQMPSHEEINKDNRTRVSIIGRPNVGKSSLVNSLIGEERMIVSDIPGTTLDAVDSVVKVNNIEYTLIDTAGIRKKSKIFQNVEKYSYLRSLTTINGSDVVLLMLDASVPISDLDTNIGGLAFEEKKPIIIIANKWDLVENKEKEILKKEDEIRAYFKYLAYAKILFVSAHDKTRITKIFTAVEDIRTALDKKIKTSVFNEVLNKAQLINPAPNFNGGRLKIYYGAQVEAYLPTFVLFVNNPDYVHFSYKRFLENQIRLQFGFEGVPMSIIFRERK"
     primer_bind     complement(1460..1476)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1543..2131)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2322..3182)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3183..3287)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 ACAACGAGCA GACCGAATAG GGTCTCAAAT GAAAAAGGGT ATCGTCGCTA TTGTTGGTCG
       61 CCCTAATGTT GGTAAAAGCT CCTTGTTTAA TCGTATCATC CGTGAGAAAA AGAGCATCGT
      121 TGAAGATACG CCGGGCGTGA CGCGCGACCG CATTTATGGT ACCGCAGAGT GGCTGACGCG
      181 TGAGTTCATC GTGATCGACA CCGGTGGTAT TACCCTGGAA GATCAACCGT TCGCCAAGGA
      241 GATCAAGGTT CAGGCCGAAA TCGCAATGGA GGAGGCCGAC GTGATCGTCT TTTTGTTAAA
      301 TCACCAAGAA GGTCTGTCAG ATGAGGACAA AATGATTGCG AAGATCCTGT ACAAGACCAA
      361 GAAGCCAATT GTTCTGGCGG TGAACAAGTA TGATAAGAAA ACCAGCGATT TCGACCAATA
      421 CGAATACATG AGCCTGGGCT TTGGTGAGCC GATTTTGATT AGCGCGACCC ATGGCATCGG
      481 TACCGGCGAT CTGCTGGATG ACATTATCCA TCAGATGCCG AGCCACGAAG AGATTAATAA
      541 AGACAACCGT ACGCGTGTAA GCATTATTGG CCGTCCGAAT GTGGGTAAGT CCAGCCTGGT
      601 TAACAGCCTG ATTGGCGAGG AACGCATGAT TGTTAGCGAC ATTCCGGGTA CCACGTTGGA
      661 CGCGGTTGAT AGCGTGGTCA AAGTGAATAA CATCGAATAC ACCCTGATTG ATACCGCCGG
      721 TATCCGCAAG AAAAGCAAAA TTTTCCAAAA TGTGGAAAAA TACTCGTATC TGCGTTCATT
      781 AACCACGATC AACGGTAGCG ACGTGGTCCT GCTGATGTTG GATGCAAGCG TGCCGATCTC
      841 TGATCTGGAT ACGAATATCG GTGGTCTGGC CTTTGAGGAG AAGAAGCCGA TTATTATCAT
      901 TGCAAATAAG TGGGATCTGG TTGAAAACAA AGAGAAAGAG ATCTTGAAGA AGGAGGACGA
      961 AATCCGTGCC TATTTTAAGT ATCTGGCGTA TGCGAAAATT CTGTTTGTGA GCGCGCACGA
     1021 TAAGACCCGC ATTACCAAAA TCTTTACGGC CGTCGAGGAT ATTCGTACCG CGCTGGACAA
     1081 GAAAATTAAG ACCTCTGTTT TTAATGAAGT TCTTAATAAG GCGCAATTGA TCAACCCGGC
     1141 GCCAAACTTC AACGGTGGTC GTCTGAAGAT CTACTACGGT GCACAAGTTG AGGCTTATCT
     1201 GCCGACGTTC GTTCTGTTCG TGAACAATCC GGATTACGTT CACTTTAGCT ATAAACGCTT
     1261 TCTGGAGAAC CAAATCCGCC TGCAATTCGG TTTTGAAGGT GTCCCGATGT CAATCATTTT
     1321 CCGCGAGCGC AAGTGAAGAG CTTAGAGACC GGACATTCGT CAGATCACCT CGCTGAGGTG
     1381 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     1441 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     1501 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     1561 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1621 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1681 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1741 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1801 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1861 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1921 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1981 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     2041 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     2101 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT
     2161 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     2221 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     2281 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     2341 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     2401 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     2461 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     2521 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     2581 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     2641 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     2701 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     2761 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     2821 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     2881 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     2941 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     3001 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     3061 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     3121 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     3181 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     3241 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT
     3301 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT
     3361 GGAGTGGGAG
//