LOCUS Exported 3575 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3575 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3541 /note="Endoribonuclease that initiates mRNA decay." /translation="MGIIIGLIIVSVALIISLCSLLYILTSKSQKVLNEKKIKEAKNERKKILSDTYREINEQKKSFEENLQFEKNKLEIAKQNIENENNLLIKERAIIIKQQEAIDVKNTDLDKRIKNYSDKREELIKRLEIISNMTSFEAKAELMKNVESKIQYEIVSQIKQAENLAHARAKELSNNIILSAMERFKTDIVNEKTTNLVKLPNDDIKGWIIGKDGRNLKTFEQLAGVEIIVDDTPEVVTISSFNPIRREIATKTLEKLLIDKRIQPIKIEKELKVQEKLIDETILEIGYQVMDELGIHDMDKELVKLVGKLKYRTSYGQNVLLHSVEVAKIASSIASELGLNAKQALRAGLLHDIGKAIDFEKTGSHVFLGVEVARKYGEDEVIINSIEAHHEDVAKESEIAVIVAIADAISASKPGARNNSIEDFIVRMKEIEKIGNSIPGISKTYAFQAGRQIRVIVDPVTTDDKDLAGILETLKNDLKNSVIIPGEITITAIREKREILVFN" /label="Mfl207" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAATCAGTTT CTTTGGCAGC CGGTCTCAAA TGGGTATTAT 2041 CATTGGTCTG ATTATCGTCA GCGTTGCCCT GATTATCTCC TTGTGTAGCC TGCTGTACAT 2101 CCTGACGAGC AAAAGCCAGA AGGTCTTGAA CGAGAAGAAG ATTAAGGAGG CGAAAAATGA 2161 GCGCAAGAAA ATCCTCAGCG ACACCTATCG TGAGATCAAC GAGCAGAAAA AGAGCTTCGA 2221 GGAGAATCTG CAGTTTGAGA AGAACAAACT GGAGATTGCT AAACAAAACA TCGAGAATGA 2281 GAACAATCTG CTGATCAAAG AGCGTGCAAT CATTATCAAA CAACAAGAAG CAATCGATGT 2341 CAAGAACACG GATTTAGACA AACGTATCAA AAACTACAGC GATAAACGTG AGGAGTTGAT 2401 CAAACGCCTG GAGATTATCA GCAATATGAC CAGCTTTGAG GCAAAAGCGG AGCTGATGAA 2461 AAACGTCGAA TCGAAGATCC AATACGAAAT CGTTTCTCAA ATTAAGCAAG CGGAAAACCT 2521 GGCCCATGCT CGTGCGAAGG AACTGAGCAA CAACATTATT CTGTCTGCCA TGGAGCGTTT 2581 TAAAACGGAC ATTGTTAATG AAAAGACCAC CAATTTAGTC AAGCTGCCGA ATGACGATAT 2641 TAAGGGTTGG ATTATCGGTA AGGATGGTCG TAATCTGAAA ACTTTCGAGC AACTGGCGGG 2701 CGTCGAGATT ATTGTCGATG ACACGCCGGA GGTCGTTACG ATCTCTAGCT TCAACCCGAT 2761 CCGCCGTGAA ATCGCGACCA AGACCCTGGA GAAACTGCTG ATCGACAAAA GAATTCAGCC 2821 GATTAAAATT GAAAAAGAGC TGAAAGTCCA GGAAAAACTG ATTGACGAAA CTATTTTGGA 2881 AATCGGTTAC CAAGTGATGG ATGAGCTGGG TATCCATGAT ATGGATAAGG AGCTGGTTAA 2941 GCTGGTGGGT AAGCTGAAAT ATCGTACCTC TTACGGCCAG AACGTGCTGC TGCACTCAGT 3001 GGAGGTTGCC AAGATCGCGA GCTCTATCGC AAGCGAGCTG GGTTTGAATG CGAAACAAGC 3061 GCTGCGCGCG GGTCTGTTGC ACGATATCGG CAAAGCGATC GACTTTGAAA AGACCGGCAG 3121 CCATGTGTTC CTGGGCGTCG AAGTCGCACG TAAGTACGGT GAGGATGAAG TTATCATTAA 3181 TAGCATTGAA GCCCATCACG AGGATGTCGC CAAGGAGAGC GAAATCGCAG TGATTGTTGC 3241 AATCGCAGAT GCGATCTCCG CGAGCAAGCC GGGTGCACGT AATAATTCAA TCGAGGATTT 3301 CATCGTTCGT ATGAAGGAGA TTGAGAAAAT CGGCAACAGC ATCCCGGGTA TTAGCAAAAC 3361 CTATGCGTTT CAGGCGGGCC GCCAGATCCG TGTTATTGTT GACCCGGTTA CCACCGATGA 3421 TAAAGATCTG GCGGGTATCC TGGAAACCCT GAAAAACGAC CTGAAGAACA GCGTTATTAT 3481 CCCGGGTGAG ATCACCATTA CCGCCATTCG CGAGAAACGT GAAATTTTGG TTTTTAACTG 3541 AAGAGCTTAG AGACCCTGTA TCATGGTTCC CTCGG //