LOCUS Exported 3025 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3025 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1063..1110 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2958..2974 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2975..3006 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..991 /label="Mfl208" /note="" /translation="MKFATIGTGWIVKEFLDAAKDFAELEYSICYSRKKETALSFLNETNNTNTRILTDLNELAKSDCDFVYIASPNGLHYEQTKLLLDNHKNVILEKPATFKTQQIIELKEIANKNKVILMEATKSVHVDELKILESFIESNNVNSAIFNLNQYSSRMPDVKQGKFNSVFDFELGKGSSFDLNVYPVELAIKLFGKVSDVVAKNVRLKNNVGIMNHSILSHENGIITSITCSKNNFQSINSQIFAEDKNVEIKNITSIDEFTINNSILRVAPLNIKISKSNAMWYEVKDFINLILNKDFEKMNYWLDITIETIRVLEIIENNN" primer_bind complement(1115..1131) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1198..1786) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1977..2837) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2838..2942) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGGAACTAT GGCATCGAGC GGTCTCAAAT GAAGTTCGCC ACCATTGGTA CCGGTTGGAT 61 CGTGAAGGAA TTTCTGGACG CAGCGAAGGA TTTTGCAGAA CTGGAATATA GCATCTGCTA 121 CAGCCGTAAG AAGGAAACCG CGCTGAGCTT CCTGAACGAA ACCAATAACA CCAACACCCG 181 CATCCTGACC GACCTGAATG AACTGGCAAA AAGCGACTGT GATTTTGTGT ATATTGCCTC 241 GCCAAATGGT CTGCATTATG AGCAAACTAA ACTGTTGTTA GATAATCACA AAAATGTTAT 301 CCTGGAAAAA CCGGCCACGT TTAAAACCCA GCAAATCATC GAGCTGAAGG AAATCGCCAA 361 TAAGAACAAA GTTATCCTGA TGGAAGCGAC TAAAAGCGTG CACGTGGACG AACTGAAGAT 421 CTTGGAGTCG TTCATCGAGA GCAACAACGT TAACTCCGCA ATCTTCAACT TGAATCAATA 481 CAGCTCGCGT ATGCCGGACG TGAAACAAGG TAAGTTTAAT AGCGTGTTCG ATTTTGAACT 541 CGGTAAAGGT AGCAGCTTTG ATTTAAACGT TTATCCGGTT GAGCTGGCGA TTAAGCTGTT 601 TGGCAAGGTT AGCGACGTGG TCGCTAAGAA TGTTCGTTTG AAGAACAATG TGGGTATTAT 661 GAACCACAGC ATTCTGAGCC ATGAAAACGG CATTATCACC AGCATTACCT GTTCCAAGAA 721 TAACTTCCAG AGCATCAACA GCCAGATTTT TGCGGAGGAT AAGAACGTGG AAATTAAGAA 781 CATTACGAGC ATTGACGAAT TCACCATCAA TAACAGCATT CTGCGTGTCG CGCCGCTTAA 841 TATCAAGATC AGCAAGTCGA ACGCCATGTG GTATGAGGTT AAAGACTTTA TTAACCTGAT 901 TCTGAATAAA GACTTCGAAA AAATGAACTA CTGGCTGGAC ATCACTATCG AAACCATCCG 961 TGTCCTGGAA ATTATCGAGA ACAATAACTG AAGAGCTTAG AGACCCACTC GATAGGTACA 1021 ACCGGCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG 1081 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG 1141 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT 1201 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 1261 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 1321 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 1381 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 1441 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 1501 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 1561 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 1621 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 1681 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 1741 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT 1801 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 1861 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 1921 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC 1981 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT 2041 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT 2101 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 2161 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT 2221 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT 2281 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC 2341 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT 2401 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG 2461 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG 2521 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT 2581 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC 2641 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT 2701 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT 2761 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG 2821 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 2881 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 2941 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT 3001 TTGACTTGGT CAGGTGGAGT GGGAG //