LOCUS       Exported     4178 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4178
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4144
                     /label="Mfl209"
                     /note="3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs."
                     /translation="MNKEIILQIVKENNEMISINSLATKLNYFDLPKLEDYLKELNEENILAFTTEKNVYLLGGSFKKGNLRMNPKGFGFVNDVLQPEEEAYFVPPVSLNGCFDSDEVIFKVVKDQEKTRAEIVELCLRVKEFLIGEIVRSFDGKFLDFIPSDQAFTGFRIRILNKKEFPIQEYQIVKAKIISVKERLLFVRLKKVIGDARKASDRILSIAEEFEIRTEFEKATLKEAERVNVPVEHEVDEINRRMKNSLLDKMVVTIDGIDSKDLDDAICVEKLENGEFKLYVAIADVSHYVEPKTPLDKEALIRGNSTYLANRVLPMLPKILSDDLCSLNPNTKKFALACEMKFDKNGIMISKEVYETIMISKVRLNYNEVNEYIANKTWNHCEESRTMIDQAIELFKLIEQVKIKRGTITFDVREPKVIMDENSNVIEIKARQTGESEKLIEQFMVSANEAVAEIVYEMELPFIYRNHDKPDEEDLITWYQSLKSFGIDPKLTPLEMLDPININKTLKRISEQTKDPIEEELLNLSLLRYMAKAKYELENIGHFGLSSKCYTHFTSPIRRYSDLIVHRYLKQYIINKDYDQKALEMNEAFIKKASVIINETETTSVDCEREVVKACTVEYMSDKIGNIYEGTISVALKFGIFVQLENMVEGLVHISNLEPNIVYDETNKILIKHDNTFYRMGQKVKIKVISADIRKRKIDFILVK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGCGCTC GGATAATACT CGGTCTCAAA TGAATAAGGA
     2041 GATCATTCTG CAGATTGTGA AAGAGAACAA CGAAATGATC AGCATTAATA GCCTGGCAAC
     2101 TAAGCTGAAC TATTTTGATC TGCCGAAACT GGAGGACTAT CTGAAAGAGC TGAACGAAGA
     2161 GAATATCCTG GCGTTTACGA CCGAGAAGAA TGTTTATCTG CTGGGTGGCA GCTTCAAAAA
     2221 AGGCAATCTG CGTATGAACC CGAAGGGTTT TGGTTTTGTT AACGATGTAC TGCAGCCGGA
     2281 GGAGGAGGCT TATTTTGTTC CGCCGGTGAG CCTGAACGGC TGCTTTGACT CTGACGAAGT
     2341 CATCTTTAAA GTTGTGAAGG ATCAGGAAAA AACCCGCGCA GAGATTGTGG AGCTGTGCCT
     2401 GCGTGTGAAG GAATTCTTGA TTGGCGAAAT TGTCCGTAGC TTCGATGGCA AATTTCTGGA
     2461 CTTCATCCCT AGCGATCAGG CTTTTACGGG TTTCCGTATC CGTATCCTGA ATAAAAAGGA
     2521 ATTTCCGATC CAAGAATACC AGATTGTCAA AGCGAAGATT ATTTCTGTCA AAGAACGTTT
     2581 GCTGTTCGTT CGTCTGAAGA AGGTTATCGG CGACGCACGT AAAGCAAGCG ATCGTATCCT
     2641 GTCCATTGCT GAAGAATTCG AGATTCGTAC CGAATTCGAG AAAGCGACGC TGAAGGAAGC
     2701 GGAACGCGTG AATGTCCCAG TTGAGCACGA GGTCGATGAA ATTAATAGAC GTATGAAAAA
     2761 CAGCCTGCTG GACAAAATGG TGGTCACGAT TGATGGCATC GATAGCAAAG ATCTGGACGA
     2821 TGCAATTTGC GTGGAGAAAC TGGAAAATGG TGAATTTAAA CTGTATGTGG CTATCGCGGA
     2881 TGTGAGCCAC TACGTTGAGC CGAAAACCCC ACTGGATAAA GAAGCCCTGA TCCGTGGTAA
     2941 TAGCACGTAT CTGGCTAATC GTGTCCTTCC GATGCTGCCG AAAATTCTGT CCGATGACCT
     3001 GTGTAGCCTG AACCCGAACA CGAAAAAATT TGCGCTGGCT TGTGAGATGA AATTTGATAA
     3061 AAATGGCATC ATGATCAGCA AGGAAGTTTA CGAAACCATT ATGATCTCCA AGGTGCGTCT
     3121 GAATTATAAC GAGGTGAACG AGTATATCGC GAATAAGACC TGGAACCACT GCGAGGAGTC
     3181 ACGCACCATG ATCGATCAAG CGATCGAGCT GTTTAAACTG ATTGAGCAAG TGAAAATTAA
     3241 AAGAGGTACC ATCACCTTCG ACGTCCGTGA ACCGAAGGTG ATTATGGACG AAAACTCGAA
     3301 CGTGATTGAG ATCAAAGCAC GCCAAACGGG CGAGAGCGAG AAGCTGATTG AGCAGTTTAT
     3361 GGTGAGCGCG AACGAGGCTG TGGCTGAGAT CGTCTATGAA ATGGAACTGC CGTTCATCTA
     3421 TCGTAATCAC GATAAGCCAG ATGAGGAGGA CCTGATTACG TGGTATCAAA GCTTAAAGAG
     3481 CTTCGGCATT GACCCGAAGC TGACCCCGCT GGAAATGCTG GACCCGATCA ACATCAATAA
     3541 GACCCTGAAG CGCATCAGCG AACAAACCAA AGACCCGATT GAGGAGGAGC TGCTCAACCT
     3601 GTCCCTGCTG CGTTATATGG CGAAAGCAAA ATATGAACTG GAAAACATCG GCCATTTTGG
     3661 TCTGAGCTCC AAATGCTACA CGCACTTCAC TAGCCCGATC CGTCGTTACA GCGATCTGAT
     3721 CGTGCACCGC TACTTGAAGC AATACATCAT CAACAAGGAC TATGATCAGA AGGCGTTGGA
     3781 GATGAACGAA GCGTTCATTA AGAAAGCGTC TGTTATCATT AACGAAACCG AAACGACGAG
     3841 CGTGGATTGC GAACGCGAGG TTGTTAAGGC ATGTACCGTG GAGTATATGT CGGATAAAAT
     3901 TGGTAACATT TATGAAGGCA CGATTTCTGT GGCGCTGAAA TTTGGCATTT TTGTACAGCT
     3961 GGAGAACATG GTCGAAGGTC TGGTGCACAT CTCTAATCTG GAACCTAACA TCGTTTACGA
     4021 CGAGACTAAT AAGATTCTGA TTAAACACGA TAACACCTTT TACCGCATGG GTCAAAAGGT
     4081 CAAAATCAAA GTTATCTCCG CAGATATTCG TAAGCGCAAA ATTGACTTCA TCTTGGTCAA
     4141 GTGAAGAGCT TAGAGACCGT ACGTCTGAAC TTGGGACT
//