LOCUS       Exported     4193 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4193
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4159
                     /label="Mfl214"
                     /note=""
                     /translation="MKSFSSIEWKSSAKNSWEMFKKGCGNIMPTLSKLSKAFLLPIALLPIAGVFLGVGSAIASNSVDGTFGYFLGSLLNKMGDVCFGNLPVLFCISVALAYTKDSGIAAITAVVGFLVMNGMQAALLHTQQLNIDTTNLWVQTSIDENGKAVWEHFTSVVVDANGKGKINWADSSINLQNIMAWGIGSSEDNLRMVNSNFELIDGAGSVYYSLLWINKIPNGLITSNIGVNSLNTGVFAGIFVGAIAAKCYNKFHQTQLPSAISFFSGTKLVPIVTFVAVIPLSFIFMFIWPYVGMGLAAFGQASGKLPGGLDSFIYEVAERSLVPFGLHHVFYAPLWWTNAGGSIAEAFNKATPEQQAEFVKIWSANHSVLAETLLNGVSEGLKFQTIRNMISSDYPDLWKSMGDQTMAYSVIAKINVLNFTDLEALGLNIGRFQSGKFGFMLLGLPMAGLAMWLNVPKENRKEVMGIYFSAAFTCFLTGITEPIEYTFLFLAPWLFYGVHMPLAAISFLFAGLFKTHVSMTVSGGFIDYIVFGVIPYFGSKAMSAKSAFAILGVSAAMAPAYFFGFYFAVKYGNVMVPGRDGSTNVQLATKAEYKASKGLNVDGSKIEEGKSTKASAKDAVEEARIQKATKIIEFLGGEANIVDVDACASRLRLTVKDGSLVDKDGIISLGGATGALIRGTNVQVVYGGEQEAIKPRMIKILDEQRKSKK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAATAAGAC GACGACCCTC GGGTCTCAAA TGAAAAGCTT
     2041 CAGCAGCATT GAGTGGAAAA GCTCTGCGAA GAATAGCTGG GAGATGTTCA AGAAGGGTTG
     2101 CGGTAACATC ATGCCGACGC TGAGCAAACT GAGCAAAGCG TTCTTGCTGC CGATCGCCCT
     2161 GCTGCCGATC GCGGGTGTGT TCCTCGGCGT CGGCAGCGCG ATCGCGAGCA ATAGCGTTGA
     2221 CGGTACCTTC GGCTATTTTC TCGGTAGCCT GCTGAATAAA ATGGGTGACG TTTGCTTCGG
     2281 CAATCTGCCG GTCCTGTTTT GTATCAGCGT TGCGTTGGCT TATACCAAGG ATAGCGGTAT
     2341 CGCGGCAATC ACGGCCGTCG TTGGTTTTTT GGTTATGAAC GGTATGCAGG CCGCGCTGTT
     2401 GCACACGCAA CAGTTGAACA TCGACACCAC CAACTTGTGG GTTCAAACGA GCATCGACGA
     2461 GAATGGTAAA GCTGTCTGGG AACACTTTAC GTCTGTGGTT GTGGACGCGA ATGGTAAAGG
     2521 CAAAATCAAC TGGGCGGATA GCAGCATCAA CTTGCAGAAT ATTATGGCCT GGGGTATCGG
     2581 TTCTTCCGAG GACAATCTGC GTATGGTCAA TAGCAATTTT GAGCTGATTG ACGGTGCTGG
     2641 TAGCGTCTAC TACTCCCTGC TGTGGATTAA CAAGATTCCT AACGGCCTGA TCACCAGCAA
     2701 CATCGGCGTT AACTCTCTGA ATACGGGTGT TTTCGCGGGT ATTTTCGTTG GCGCCATTGC
     2761 TGCTAAGTGT TACAACAAGT TCCACCAGAC GCAATTGCCG TCCGCAATCA GCTTTTTCAG
     2821 CGGCACCAAA CTGGTGCCGA TCGTCACGTT CGTGGCAGTT ATCCCGCTGA GCTTTATTTT
     2881 TATGTTTATT TGGCCATACG TTGGTATGGG TCTGGCGGCA TTCGGCCAGG CCAGCGGCAA
     2941 GCTGCCGGGT GGTCTGGACT CCTTCATCTA TGAAGTTGCG GAGCGCAGCC TGGTCCCGTT
     3001 TGGCCTGCAT CACGTGTTCT ACGCGCCGCT GTGGTGGACC AACGCGGGTG GCTCTATCGC
     3061 CGAGGCTTTC AACAAGGCAA CGCCGGAGCA ACAGGCAGAA TTTGTTAAAA TCTGGAGCGC
     3121 AAACCACTCC GTGCTGGCCG AAACCCTGCT GAACGGTGTC AGCGAGGGCC TGAAATTCCA
     3181 GACGATCCGC AATATGATTT CTTCCGACTA CCCGGATTTG TGGAAAAGCA TGGGTGATCA
     3241 GACGATGGCT TATAGCGTTA TTGCCAAGAT CAACGTTCTG AACTTCACTG ACCTGGAGGC
     3301 GCTGGGCCTG AATATTGGCC GTTTCCAGAG CGGCAAATTT GGTTTTATGC TGCTGGGTCT
     3361 GCCAATGGCT GGTCTGGCAA TGTGGCTGAA CGTCCCGAAG GAGAATCGCA AAGAGGTCAT
     3421 GGGCATCTAC TTCAGCGCGG CTTTTACGTG CTTCTTGACG GGCATTACGG AGCCGATTGA
     3481 GTATACCTTC CTGTTCCTGG CGCCGTGGTT GTTCTACGGC GTGCACATGC CGTTGGCGGC
     3541 GATTAGCTTT CTGTTTGCGG GTCTGTTCAA AACCCACGTG AGCATGACGG TCAGCGGTGG
     3601 TTTTATCGAC TACATCGTCT TTGGTGTTAT TCCGTACTTC GGTAGCAAAG CTATGAGCGC
     3661 AAAAAGCGCA TTCGCAATCC TGGGCGTTAG CGCCGCCATG GCACCAGCGT ACTTTTTTGG
     3721 TTTTTACTTT GCTGTCAAGT ACGGTAACGT TATGGTTCCT GGCCGTGACG GTTCCACCAA
     3781 TGTGCAATTG GCCACGAAAG CCGAGTATAA AGCGTCCAAA GGCTTGAACG TGGATGGTAG
     3841 CAAGATCGAG GAGGGTAAAA GCACCAAGGC GAGCGCCAAA GATGCAGTGG AGGAAGCGCG
     3901 CATTCAAAAG GCAACCAAAA TCATTGAGTT TCTGGGTGGT GAGGCGAACA TCGTTGACGT
     3961 CGATGCGTGT GCGAGCCGCT TACGTCTGAC CGTGAAAGAT GGTTCTCTGG TCGACAAAGA
     4021 CGGCATTATT TCACTGGGCG GCGCAACCGG CGCGTTAATC CGTGGTACGA ATGTTCAGGT
     4081 GGTCTATGGT GGCGAACAGG AGGCGATCAA GCCGCGTATG ATTAAAATTC TGGACGAGCA
     4141 ACGTAAGAGC AAAAAGTGAA GAGCTTAGAG ACCCCCCACT GTTAGATTGA GCT
//