LOCUS       Exported     3469 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3469
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1507..1554
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3402..3418
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3419..3450
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1435
                     /note="Acts as a magnesium transporter."
                     /translation="MLKLDHIEEMLEKALEEKDVKKIRSISKEFQFVDFAEAMEQFESKKVLKIFRLLELEEAAEIFTYLDSEHQEYIIEAFTNTEIQEILDELYTDDIVDLIDEMPSEVVKKILKATTKDVRKELNNILKYKEHTAGAIMSINFTELKADNTVEMAIKAIKRRHEDYDEIDDLFIIDEQNKLLGWLELKQLLINTPETKLEKIMNSKIISVNVEMDQEEVANYFKRYDINTLPVVNKNQQLVGIITVDDVIDVLVEESTEDIQNFAGIDADNVESDYFETSIFKMYKSRIVWLSILLLIGIVTHVIMLIMFNLVDGLKNLSSGREMIMMIPILIVLAGVSGNIANQSSLMMIRSLALNQVHKKEIKTIFIKELIVGFLLGITLSFINVIRLLIIYAIQGGGSINHMEWMVILFSTLGILVVVMLANLLGILLPLILKKIKRDPTIASSPLITSLVDIMCVLVFIGFALIIV"
                     /label="Mfl217"
     primer_bind     complement(1559..1575)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1642..2230)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2421..3281)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3282..3386)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCCCAGA AGGATACAAC GGTCTCAAAT GCTGAAGCTG GACCATATTG AAGAAATGCT
       61 GGAGAAGGCG CTGGAAGAAA AGGACGTGAA GAAAATTCGT AGCATCAGCA AGGAATTCCA
      121 GTTCGTTGAC TTCGCAGAGG CAATGGAGCA ATTCGAGAGC AAAAAAGTGC TGAAAATCTT
      181 CCGTCTGCTG GAACTGGAAG AGGCTGCGGA AATCTTCACG TACCTGGATA GCGAACATCA
      241 GGAGTACATC ATCGAGGCGT TCACCAACAC TGAGATTCAG GAGATTCTGG ATGAGCTGTA
      301 TACGGACGAC ATTGTCGACC TGATTGATGA GATGCCGAGC GAAGTGGTGA AGAAAATCCT
      361 GAAGGCGACC ACCAAAGACG TGCGTAAGGA GCTGAATAAC ATCCTGAAGT ACAAAGAGCA
      421 CACCGCAGGC GCTATCATGA GCATCAATTT TACCGAGCTG AAAGCAGACA ACACTGTCGA
      481 AATGGCGATT AAGGCTATCA AGCGTCGTCA CGAGGACTAT GACGAGATCG ACGATCTGTT
      541 CATTATTGAC GAGCAGAATA AGTTGCTGGG TTGGCTGGAG CTGAAGCAGC TGCTGATTAA
      601 TACCCCGGAA ACTAAACTGG AGAAAATCAT GAATTCCAAA ATCATTAGCG TTAACGTGGA
      661 GATGGACCAG GAGGAGGTCG CGAACTACTT CAAGCGTTAC GATATTAATA CCCTGCCGGT
      721 CGTGAACAAG AACCAACAGC TGGTTGGTAT TATTACCGTC GACGACGTCA TTGACGTTTT
      781 GGTGGAGGAG TCTACGGAGG ACATCCAGAA TTTCGCCGGT ATTGATGCGG ACAATGTCGA
      841 GTCCGACTAC TTTGAAACCA GCATCTTTAA GATGTACAAG TCTCGTATCG TGTGGCTGTC
      901 CATTCTGTTA CTCATCGGTA TCGTCACCCA CGTTATCATG TTGATCATGT TTAACCTGGT
      961 GGACGGTTTG AAGAATCTGA GCAGCGGTCG TGAGATGATC ATGATGATTC CGATCCTTAT
     1021 TGTGCTGGCC GGCGTGTCCG GTAATATCGC GAATCAAAGC AGCTTAATGA TGATTCGTAG
     1081 CCTGGCTCTG AACCAGGTTC ACAAGAAAGA AATCAAAACC ATCTTCATTA AAGAACTGAT
     1141 TGTGGGTTTT TTGCTGGGTA TTACCCTGAG CTTCATCAAT GTTATCCGTT TACTGATTAT
     1201 TTATGCTATC CAGGGTGGTG GCAGCATTAA CCACATGGAG TGGATGGTCA TTTTGTTCAG
     1261 CACCCTGGGT ATCCTGGTAG TGGTCATGTT GGCCAATCTG CTGGGTATTC TGCTGCCGCT
     1321 GATCTTAAAA AAGATTAAAC GTGATCCGAC CATTGCCAGC AGCCCGCTGA TCACCAGCCT
     1381 GGTTGACATT ATGTGTGTGC TGGTGTTCAT CGGTTTTGCA CTGATCATTG TGTGAAGAGC
     1441 TTAGAGACCG CAGCGATTCG GATACTACTC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
     1501 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1561 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1621 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1681 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1741 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1801 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1861 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1921 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1981 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     2041 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     2101 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     2161 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     2221 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     2281 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     2341 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     2401 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     2461 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     2521 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     2581 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     2641 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     2701 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     2761 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     2821 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     2881 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     2941 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     3001 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     3061 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     3121 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     3181 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     3241 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     3301 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     3361 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     3421 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGGGAG
//