LOCUS       Exported     3710 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3710
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3676
                     /label="Mfl229"
                     /note=""
                     /translation="MEKLILIKDSMTSAVNNLYNNYPHIDKLNVFPVPDGDTGTNMNLTATNGYNDVKDIEFKTIGEFLNAFSRGLIMGARGNSGVIFSQIIKGLAKGMNDASELSAAEWKKGFAESKIIAYRAVMKPVEGTILTVIREVAEQSALLPDDMEIKEFWNKIILIANEALENTPNLLQALKDVGVVDSGAYGLVKFLEGMNSVFQSNKIIAKTDKLEINEGGNIEMEIEAEFGYCTEGIVMLNEEWINKLQTSAIRDQLQIYGNTSIVVVIDEDILKVHTHALSPGQVLMFLQQYGDFKTIKVDNMNLQADKQVKGNEASGWQETTSIKLERKLNNDYATIAVVSSPEMKKYFEKELGIDIAIDGGSKMNPSTNDFLKAIEEVDAKAVYLMPNNGNVLLAAKQAEKEETKSKIIVIPTKTIQQGMTAALSFDPSATTMKNTKAITSAIKNVISFQVSQAAKDSVVNGIKIKKDQQMAIVDGKIVGTANDIGILFEKQLSRYITNKTEIITIFIGQDASAKSVSQLRKFLDENFDVEYEIIEGGQKVYSFIIAVE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGGGGCC GTCAATATCA GGGTCTCAAA TGGAGAAGCT
     2041 GATCCTGATC AAAGACAGCA TGACGAGCGC GGTCAATAAT CTGTACAATA ATTATCCGCA
     2101 TATCGACAAA CTGAACGTTT TCCCAGTGCC TGATGGCGAT ACCGGCACCA ACATGAATTT
     2161 GACGGCGACC AACGGTTATA ACGACGTCAA GGACATTGAA TTCAAAACTA TCGGTGAGTT
     2221 TCTGAATGCG TTCAGCCGTG GCCTGATTAT GGGCGCGCGT GGCAACTCTG GTGTCATCTT
     2281 CAGCCAAATT ATTAAGGGTC TGGCGAAAGG TATGAATGAC GCAAGCGAGC TGAGCGCAGC
     2341 GGAATGGAAG AAGGGTTTTG CGGAGAGCAA AATTATCGCG TATCGTGCCG TTATGAAACC
     2401 GGTCGAGGGT ACGATTCTGA CCGTTATCCG CGAGGTGGCG GAGCAGAGCG CGCTGCTGCC
     2461 GGACGATATG GAAATCAAAG AGTTCTGGAA CAAGATTATC CTGATCGCGA ATGAAGCACT
     2521 GGAGAATACC CCGAATCTGC TGCAAGCTCT GAAAGATGTG GGCGTAGTTG ATTCTGGCGC
     2581 CTACGGCTTG GTCAAATTCT TGGAGGGTAT GAACTCAGTC TTTCAATCCA ATAAAATTAT
     2641 TGCAAAGACC GATAAGCTGG AAATCAACGA GGGCGGCAAT ATCGAGATGG AGATTGAGGC
     2701 TGAGTTCGGC TATTGTACCG AGGGTATTGT CATGCTGAAT GAGGAATGGA TCAATAAGCT
     2761 GCAAACCAGC GCTATTCGTG ATCAACTGCA GATCTACGGT AACACCAGCA TCGTGGTCGT
     2821 TATCGACGAG GACATCCTGA AGGTTCATAC CCATGCGCTG AGCCCTGGCC AGGTGCTGAT
     2881 GTTTTTGCAG CAATATGGTG ATTTCAAAAC GATCAAAGTT GATAACATGA ATCTGCAGGC
     2941 GGACAAACAA GTCAAGGGTA ACGAGGCCAG CGGTTGGCAG GAAACGACGA GCATTAAGCT
     3001 GGAGCGTAAG CTGAATAATG ACTACGCGAC GATCGCGGTG GTTTCTTCCC CGGAAATGAA
     3061 AAAATACTTT GAAAAAGAGC TGGGTATTGA TATTGCGATT GACGGCGGTA GCAAAATGAA
     3121 TCCGTCTACC AACGATTTTC TGAAGGCCAT CGAAGAGGTT GATGCGAAGG CGGTGTATCT
     3181 GATGCCGAAT AACGGCAACG TTCTGCTGGC GGCGAAGCAG GCTGAGAAAG AAGAAACGAA
     3241 AAGCAAAATC ATCGTGATCC CGACCAAGAC GATCCAACAG GGTATGACCG CGGCGCTGAG
     3301 CTTTGATCCA AGCGCGACCA CGATGAAGAA TACCAAAGCG ATCACGAGCG CGATCAAAAA
     3361 CGTCATCAGC TTCCAAGTCA GCCAAGCCGC CAAAGACAGC GTGGTGAACG GTATTAAAAT
     3421 TAAAAAGGAT CAACAAATGG CAATTGTCGA CGGTAAAATT GTTGGCACCG CCAACGATAT
     3481 CGGCATCCTG TTTGAGAAAC AATTAAGCCG CTATATCACC AATAAAACGG AGATTATCAC
     3541 CATTTTTATT GGCCAGGACG CCAGCGCTAA GAGCGTGTCT CAGCTGCGTA AATTTCTGGA
     3601 CGAAAATTTC GACGTCGAGT ATGAGATTAT TGAAGGTGGT CAGAAAGTTT ACTCCTTCAT
     3661 TATCGCCGTC GAGTGAAGAG CTTAGAGACC GTAGTGCCTG TTCGTTCTGT
//