LOCUS Exported 3059 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3059 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3025 /label="Mfl230" /note="Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA." /translation="MYKIAFDVMGSDNGSAVAIEAASRFIKSRKDLYLVFVGDEDQIKTSLEKFPIDESRFEILATKEFIDMNGSIMDIRRKKDSSMVRALETLKDKKVDAMITGGNSAAFIAGSHFILGELNGISRPGFMPTLPTAVSNKLTLLLDVGANLEADIEDIIGYAKMANIYAKNVLKIENPLIAQLNIGEEKSKGTLLQKEIYKELESDENINFFGNLESRDILAGKVDIIVTDGYTGNMCLKAFEGASKILMTEIKSQLYKTIFTKLKALTLKKSFDNVSKKFDYKNHSGAILLGVEGIAFKAHGSSDVKSFEATLRMTCDAVENDVLNKIKKELN" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAGACGATC CGAGCCATTA CGGTCTCAAA TGTACAAGAT 2041 CGCGTTTGAC GTGATGGGTT CTGATAACGG TAGCGCTGTG GCGATCGAAG CCGCCAGCCG 2101 TTTTATCAAG AGCCGTAAAG ATCTGTACCT GGTCTTTGTA GGTGACGAGG ACCAGATTAA 2161 AACCTCGCTG GAGAAATTTC CGATCGACGA ATCCCGCTTC GAGATTCTGG CCACGAAAGA 2221 ATTCATTGAT ATGAACGGCA GCATCATGGA TATCCGTCGT AAGAAGGATT CGTCGATGGT 2281 GCGCGCCTTG GAAACGCTGA AGGACAAGAA GGTTGACGCC ATGATCACCG GCGGTAACTC 2341 TGCTGCATTT ATCGCCGGTA GCCACTTTAT TCTGGGCGAA CTGAACGGTA TTTCCCGTCC 2401 TGGTTTCATG CCGACTTTGC CGACGGCAGT CTCCAACAAG CTGACCCTGT TGCTGGACGT 2461 AGGTGCGAAC TTAGAAGCGG ATATCGAAGA TATCATTGGT TACGCGAAGA TGGCGAACAT 2521 TTACGCGAAG AACGTTCTGA AAATTGAAAA TCCACTGATT GCTCAACTGA ATATTGGTGA 2581 GGAAAAGTCT AAGGGTACGT TGCTGCAGAA GGAGATCTAT AAAGAATTGG AGAGCGACGA 2641 AAATATTAAC TTTTTCGGCA ACCTGGAGTC CCGTGACATC CTGGCGGGTA AGGTCGATAT 2701 TATCGTTACC GACGGTTACA CCGGCAACAT GTGCCTGAAA GCATTCGAGG GCGCAAGCAA 2761 GATTCTGATG ACCGAGATCA AGAGCCAGCT GTACAAAACC ATTTTCACCA AACTGAAGGC 2821 GCTGACGTTG AAGAAAAGCT TTGACAACGT TAGCAAGAAG TTTGACTATA AAAATCACTC 2881 TGGTGCAATT CTGCTGGGCG TCGAGGGTAT TGCGTTTAAA GCCCACGGCA GCTCGGATGT 2941 CAAGAGCTTT GAAGCCACCC TGCGCATGAC CTGTGATGCA GTGGAAAACG ACGTCCTGAA 3001 CAAGATTAAG AAAGAGCTGA ACTGAAGAGC TTAGAGACCC CAGTATCGGC AGCTAATCT //