LOCUS       Exported     5051 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..5051
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..5017
                     /note="Required for chromosome condensation and partitioning."
                     /translation="MLFLKQIRAVGFKSFAEPTTLNFTKEMIGVVGPNGSGKSNITDSIRWALGEQSTKSLRGANMDDIVFSGSTDKPAADFAEVTLVFDNQRDIFSTIKTDVVEITRRFNKKTRDSDFFINGEKCKLRDIQDVALETGLTKSSIAIISQGTISTFAEAKPDARREIFDEAAGLAKYKKRKLEALKQLAKTTENLTRISDIKLTLEKRLPREKEKAEKAAKYKDKIEELQKIELTILASDALRFETELSSLRDKRRQLDIEVQKLANEINLSQDELDVMLSKNGDADKEITQLNLNFQRIVERIANLKNQKQQVEARENSENVNENIDDIKARAIKKEFDEKSISLNSEKDLIISLERQELDLKKRYDEVNDQFRTFHMQSQEIESEKNKLQYRLEELEHKQNTNNLNPMSGAKAIIDNAKRLSGVVGTVGSLIDVKEEHQIAISLITGNHLQSVVFKTSDDAKKGIEFLKNQRLGRVNALPIDTLNPSSIAGPQRDIIKRAPGFVGFANELVEIEKDCQVVLDYIYGTTIVTRNFDDATRLGKSINFRYGIVSLDGQRVLPRGAMSGGSVNKASNIFAAKKVDESFDPESIKNKIITLDKIFNEKQKTFNDLKEVREKLIDDINQASSNIRIGKNSINILNSSLVELSDNYKIITGKDLLNNQTTSSFDESESIRLAREIAKLETERNEISIKVNALSDSKTKTTDRQHELNKENKEKRETLNNWKDELANVKSDLTILESTNIQILKRLSEGYNLSLDAIREMHFDEIENPEETRTRIQELTIELKSIGEVSMDAIQEYEETKKEYDYYVTNLNEVQESADKLNEIILNIDIEMKTQFKRIVDDVNAALPEAFQKLFNGGTASLIYTNPDDILETGIDIEVNPPGKKITNLNLLSGGEKSLVALSVLFSILKVRPLPLVILDEAEAPLDPANVTRFARYVRDFVENTQFIIVTHREGTMENCDILYGVTMETKGITKIVQLALDIDKIKKLINKNKE"
                     /label="Mfl232"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACACGTG GCAAACATTC CGGTCTCAAA TGCTGTTCCT
     2041 GAAACAGATC CGTGCCGTCG GCTTCAAGAG CTTCGCGGAG CCGACCACCC TGAACTTTAC
     2101 GAAAGAAATG ATTGGTGTGG TTGGCCCAAA TGGCTCTGGC AAATCTAATA TCACGGACTC
     2161 TATTCGCTGG GCGCTGGGCG AGCAGAGCAC CAAGAGCCTG CGTGGCGCAA ACATGGATGA
     2221 TATCGTTTTT AGCGGTAGCA CCGATAAGCC GGCAGCGGAC TTCGCGGAGG TTACGCTGGT
     2281 GTTCGATAAC CAGCGCGATA TCTTTTCCAC CATCAAAACG GATGTGGTTG AGATCACGCG
     2341 CCGCTTCAAC AAAAAGACGC GTGACAGCGA CTTCTTTATT AATGGCGAAA AGTGTAAGCT
     2401 GCGCGATATC CAGGATGTCG CGCTGGAAAC GGGCCTGACC AAGAGCTCTA TTGCGATCAT
     2461 CTCTCAGGGC ACTATCAGCA CGTTTGCCGA GGCGAAGCCG GACGCGCGCC GTGAAATCTT
     2521 CGATGAGGCT GCGGGCCTGG CGAAATACAA AAAGCGTAAA TTGGAGGCTC TGAAGCAGCT
     2581 GGCGAAAACC ACCGAAAATC TGACCCGTAT CTCTGATATC AAGTTAACCC TGGAGAAGCG
     2641 TCTGCCTCGT GAGAAAGAGA AGGCGGAGAA GGCGGCGAAA TACAAGGATA AGATTGAAGA
     2701 ACTGCAGAAA ATTGAACTGA CCATCCTGGC GTCTGACGCC CTGCGTTTCG AAACGGAACT
     2761 GTCTAGCCTG CGTGATAAAC GTCGCCAACT GGACATCGAG GTGCAGAAAC TGGCCAACGA
     2821 AATTAACCTG AGCCAGGACG AACTGGATGT TATGCTGAGC AAGAACGGTG ATGCGGACAA
     2881 GGAGATTACT CAATTAAATC TGAATTTTCA ACGCATCGTG GAACGTATTG CCAATTTGAA
     2941 GAATCAAAAG CAACAAGTTG AGGCACGCGA GAATTCCGAA AACGTGAACG AGAACATTGA
     3001 TGATATTAAG GCGCGTGCGA TCAAGAAGGA ATTTGATGAA AAGAGCATCT CCCTGAATAG
     3061 CGAGAAAGAT CTGATCATTA GCTTGGAGCG TCAGGAACTG GACCTGAAGA AACGTTATGA
     3121 TGAGGTCAAT GACCAGTTCC GTACCTTCCA TATGCAAAGC CAGGAGATCG AGTCGGAGAA
     3181 GAATAAACTG CAGTATCGTC TGGAGGAGCT GGAGCACAAG CAAAACACCA ACAACCTGAA
     3241 TCCGATGAGC GGCGCGAAAG CAATCATCGA TAATGCGAAA CGTCTTAGCG GTGTGGTGGG
     3301 CACCGTCGGT TCTTTGATCG ACGTTAAGGA GGAGCACCAG ATCGCGATCA GCCTGATTAC
     3361 TGGCAACCAC TTGCAAAGCG TTGTGTTTAA AACCTCAGAT GACGCGAAAA AGGGCATTGA
     3421 ATTTTTAAAA AACCAGCGTT TGGGTCGCGT GAATGCGCTG CCAATTGATA CCTTAAACCC
     3481 GAGCAGCATT GCTGGTCCTC AGCGTGATAT TATCAAGCGC GCGCCGGGCT TTGTAGGCTT
     3541 CGCGAATGAG CTGGTCGAAA TTGAAAAGGA CTGCCAGGTC GTTCTGGACT ACATCTACGG
     3601 CACCACCATC GTCACCCGCA ATTTTGATGA CGCTACCCGT TTGGGTAAGA GCATCAACTT
     3661 CCGCTACGGC ATCGTGAGCC TGGACGGCCA GCGTGTCCTG CCGCGTGGTG CAATGAGCGG
     3721 CGGTAGCGTC AATAAGGCAA GCAATATTTT CGCTGCGAAA AAAGTTGACG AATCCTTTGA
     3781 TCCTGAAAGC ATCAAAAACA AGATTATCAC GCTGGATAAG ATCTTTAATG AGAAACAGAA
     3841 AACCTTCAAC GACCTGAAGG AAGTTCGTGA GAAGTTGATT GATGATATCA ACCAAGCATC
     3901 TAGCAACATC CGTATTGGTA AAAATAGCAT CAACATTCTG AATTCGAGCC TGGTGGAGCT
     3961 GAGCGACAAT TATAAAATCA TCACCGGTAA GGACTTATTG AACAACCAAA CCACCAGCTC
     4021 TTTCGACGAA TCGGAAAGCA TCAGACTGGC GCGCGAGATC GCGAAACTGG AAACCGAACG
     4081 CAACGAAATT TCGATCAAAG TCAACGCGTT GAGCGACTCA AAGACCAAAA CCACCGACCG
     4141 TCAACATGAA CTGAATAAGG AAAATAAAGA GAAGCGTGAA ACTTTGAATA ACTGGAAGGA
     4201 CGAACTGGCG AATGTCAAAA GCGATCTGAC CATTCTGGAA AGCACCAATA TTCAGATCTT
     4261 AAAGCGTCTG TCTGAGGGCT ACAATCTGAG CCTGGATGCG ATCCGCGAGA TGCATTTCGA
     4321 CGAGATTGAA AACCCGGAAG AGACTCGCAC GCGTATCCAG GAGCTGACGA TTGAATTAAA
     4381 GAGCATCGGT GAGGTAAGCA TGGATGCCAT TCAGGAATAC GAGGAGACTA AGAAGGAATA
     4441 CGATTACTAC GTCACCAATC TGAACGAAGT GCAAGAGAGC GCCGATAAAT TGAATGAAAT
     4501 CATCCTGAAC ATCGACATCG AGATGAAAAC TCAGTTTAAA CGTATCGTCG ACGATGTTAA
     4561 CGCAGCGTTA CCGGAAGCGT TTCAGAAACT GTTCAATGGT GGTACCGCGA GCCTCATCTA
     4621 TACCAATCCT GATGACATTT TGGAGACTGG CATTGACATT GAGGTGAACC CGCCAGGTAA
     4681 GAAAATCACG AATCTGAATC TGCTGAGCGG TGGTGAAAAG AGCTTGGTGG CGCTGAGCGT
     4741 CCTGTTCAGC ATTTTAAAGG TGCGTCCACT GCCGCTGGTG ATTCTGGACG AGGCGGAAGC
     4801 GCCGTTAGAT CCGGCAAATG TGACCCGTTT CGCCCGCTAT GTCCGTGACT TTGTGGAGAA
     4861 TACCCAGTTT ATCATCGTCA CCCACCGTGA GGGCACGATG GAGAATTGTG ATATTCTGTA
     4921 TGGTGTTACC ATGGAGACTA AAGGTATCAC CAAGATCGTT CAACTGGCGC TGGATATTGA
     4981 CAAGATCAAG AAACTGATTA ATAAGAACAA AGAGTGAAGA GCTTAGAGAC CGTACTGTAT
     5041 GGCTCCGGTT T
//