LOCUS       Exported     3202 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3202
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1240..1287
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3135..3151
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3152..3183
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1168
                     /label="Mfl233"
                     /note=""
                     /translation="MNKKFFLVMVIVVGAIVGLWSWTLVAPNNSISIGGSASVQPLLKKLTDKYKTEDGKKFVYSATGSGAGVTNVKEGVYEIGFISKDIGDNDFTPLPINENKTLFKDLTEENINDKHWYSNTLASTQGTEETYRSIEFARDSIVFVYNDQGTGFDKFLKDSNLEFVFEVNDEGKFDQKDNAQGKNINRSYKILNEIYNNDSQNDLITWNRLAILIAENFSEEGTKEQNIKLAQQVSSTAKVTPYSSTSGSGTRTSFTNLTGINPGNAVKEYGANGTIYSQIEKSPGSIGFVSMLYGSANSSRVKSVKIKQGDTTWDPSKDDSEKLSSYPLTRPFIAIYKYDTNNKDLNSILDFLFWMAASPEVKNLYSSVGLTQLVQPTKK"
     primer_bind     complement(1292..1308)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1375..1963)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2154..3014)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3015..3119)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GAACAAAAAA TTCTTCCTGG TGATGGTGAT
       61 CGTCGTCGGT GCAATCGTCG GTTTATGGTC CTGGACGTTA GTCGCTCCGA ACAACAGCAT
      121 CAGCATTGGT GGTAGCGCCT CCGTTCAGCC GCTGTTGAAA AAATTGACGG ACAAGTATAA
      181 AACTGAGGAC GGTAAGAAGT TTGTCTACTC TGCGACGGGT AGCGGCGCGG GTGTTACTAA
      241 CGTGAAAGAG GGTGTTTATG AAATTGGCTT CATTTCGAAG GACATTGGCG ACAATGACTT
      301 CACGCCTCTG CCGATTAATG AAAATAAAAC TCTGTTTAAA GATCTGACGG AAGAGAACAT
      361 CAACGACAAA CATTGGTACT CTAATACCCT GGCGTCGACC CAAGGTACGG AGGAAACCTA
      421 CCGTAGCATT GAGTTTGCGC GCGATAGCAT TGTGTTCGTC TACAATGATC AAGGCACGGG
      481 TTTCGACAAA TTCCTGAAAG ATAGCAACCT GGAGTTCGTG TTTGAAGTCA ACGACGAAGG
      541 TAAGTTTGAC CAAAAAGATA ATGCACAAGG TAAAAACATC AATCGTAGCT ATAAGATCCT
      601 GAACGAAATT TATAATAACG ATAGCCAAAA TGACCTGATC ACCTGGAATC GTCTGGCGAT
      661 TCTGATCGCT GAAAACTTCT CCGAAGAGGG CACCAAGGAA CAAAACATTA AGCTGGCGCA
      721 ACAAGTGTCC TCTACCGCGA AGGTGACCCC GTACTCCAGC ACGAGCGGCA GCGGTACCCG
      781 TACCAGCTTT ACCAACCTGA CGGGTATCAA TCCTGGTAAT GCTGTGAAAG AATACGGTGC
      841 AAATGGCACC ATTTACAGCC AGATTGAAAA GAGCCCGGGT TCTATCGGTT TCGTGTCCAT
      901 GCTGTATGGT AGCGCAAATT CTAGCCGCGT TAAAAGCGTC AAGATTAAAC AGGGCGACAC
      961 CACCTGGGAC CCGAGCAAAG ACGATAGCGA GAAACTCTCT TCCTACCCGC TGACGCGTCC
     1021 GTTCATTGCG ATTTATAAAT ATGACACCAA CAACAAAGAT TTGAACAGCA TTCTGGACTT
     1081 TTTGTTCTGG ATGGCGGCAT CTCCGGAGGT TAAGAATCTG TACAGCTCTG TCGGTCTGAC
     1141 TCAATTAGTT CAACCTACCA AGAAGTGAAG AGCTTAGAGA CCGTATGTCC CCTAAGCTCT
     1201 GGCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
     1261 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
     1321 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
     1381 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     1441 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     1501 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1561 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1621 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     1681 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     1741 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     1801 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     1861 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     1921 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC
     1981 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     2041 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     2101 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     2161 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     2221 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     2281 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     2341 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     2401 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     2461 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     2521 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     2581 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     2641 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     2701 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     2761 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     2821 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     2881 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     2941 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     3001 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     3061 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     3121 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG
     3181 ACTTGGTCAG GTGGAGTGGG AG
//