LOCUS Exported 4210 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4210 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 2248..2295 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 4143..4159 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 4160..4191 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..2176 /translation="MFLNKKSEDNGFKPKTFKPKQNFNAKFFKSLIYTLTFTVLAILFILVLFVILKSLHVFEEQGFWSFITGTNWKPGKDGEGQYGIGLIIIMTIVLLTISMLFAIPLTIFTTLFISEYLSVGMQKKVLTVIKLLASVPSVVFGLFARDQIGALFQLMGAPNNDNLMVASMTMTFMAAPTMISLSYNAVQSVPEGYRLGSLGLGISKEQTTFKIIRKSASAKIISAIILGMARVIGETMAIMMIAGNSTGGFITNSGISGFLFSSIRTLAATIGLEMTENSGSTHQSALYAIGLILFLLVFIINIVILFMSNVDNIKYSRRIKREEKLNSNSSRKIKTPKYVYDKKMLGMMVHNRTENKFFKKTYSAVMLFLMWISTAIIISFTFWILGDVIIKGLMGLSDPIAFIHMDTENRTGGGIFAALFTTILLVVCTLIFAIPFALGAAIYLNEYARQSSVLTKSFRFAINLLASTPSIVFGIFGLSIFIVLLGLPFSILAAGLTMTVVVLPMLISNFEDALQQVPHQYREAGSALGLTKIQTLFKIILPNAMEGIITGIILAMAKIIGESAPIYLTLGTDIQMPTQGFLSQGTTLTTGIYMMVAEGIPGHGQGTIYLMALITIILIIGLNFTSGRLSSLLVQKSKDLKAKSKIKRKEFNVKTKAKIKKMKPSLKFKWMKLQSKFRKGEIKITFFKEMASKNKLWVKRRKEYRKLKKGVIDHE" /label="Mfl234" /note="" primer_bind complement(2300..2316) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(2383..2971) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3162..4022) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(4023..4127) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGAATTACT GACCCCTCGG GGTCTCAAAT GTTTCTGAAT AAGAAAAGCG AAGATAACGG 61 TTTTAAGCCG AAAACCTTCA AACCTAAGCA GAATTTTAAC GCCAAGTTTT TTAAGAGCCT 121 GATTTACACG CTGACCTTCA CTGTACTGGC GATTCTGTTT ATCCTGGTCC TGTTTGTCAT 181 CCTGAAGTCT CTGCATGTTT TCGAGGAACA AGGTTTCTGG TCCTTCATTA CGGGCACGAA 241 CTGGAAACCG GGCAAAGATG GTGAGGGCCA GTATGGTATT GGCCTGATTA TCATTATGAC 301 GATTGTGCTG CTGACGATTT CTATGTTGTT TGCTATTCCG CTGACCATTT TTACCACTCT 361 CTTCATTAGC GAGTACCTGA GCGTCGGTAT GCAAAAGAAA GTTCTGACCG TCATTAAACT 421 GTTGGCCAGC GTCCCGAGCG TTGTGTTCGG CCTGTTTGCT CGCGATCAGA TTGGTGCACT 481 GTTCCAACTG ATGGGCGCGC CTAATAATGA CAATCTGATG GTGGCGAGCA TGACGATGAC 541 GTTCATGGCT GCACCGACCA TGATTTCGCT GTCTTATAAT GCGGTTCAGA GCGTGCCGGA 601 AGGTTATCGT CTTGGTTCCC TGGGTTTAGG CATCAGCAAA GAGCAAACCA CCTTTAAAAT 661 CATCCGCAAG TCCGCAAGCG CGAAGATCAT TAGCGCGATT ATTCTGGGCA TGGCACGTGT 721 TATCGGCGAA ACCATGGCGA TCATGATGAT TGCGGGTAAT TCTACGGGCG GTTTCATCAC 781 CAACTCCGGC ATTTCCGGCT TTCTGTTTTC TTCGATCCGT ACGCTGGCAG CAACGATTGG 841 CCTGGAAATG ACGGAGAACA GCGGTAGCAC CCATCAGTCT GCACTGTACG CAATTGGCCT 901 GATTCTGTTC CTGCTGGTTT TTATCATTAA TATCGTAATT CTGTTTATGA GCAACGTGGA 961 CAACATTAAG TACAGCCGCC GCATCAAGCG TGAGGAGAAA CTTAATAGCA ACAGCAGCCG 1021 TAAGATTAAG ACCCCAAAGT ATGTGTATGA CAAGAAAATG CTTGGTATGA TGGTGCACAA 1081 CCGTACGGAA AATAAATTTT TTAAAAAGAC CTATAGCGCC GTTATGCTGT TCCTGATGTG 1141 GATTAGCACC GCTATCATCA TTAGCTTCAC TTTCTGGATT CTGGGTGATG TCATCATTAA 1201 AGGTCTGATG GGTCTGAGCG ACCCGATTGC TTTCATTCAT ATGGACACGG AAAATCGTAC 1261 CGGTGGCGGT ATCTTTGCCG CGCTTTTCAC GACCATTCTG CTGGTCGTGT GTACGCTGAT 1321 CTTCGCCATC CCGTTTGCAC TGGGTGCGGC GATCTACCTG AATGAGTACG CCCGCCAGAG 1381 CAGCGTCCTG ACCAAGAGCT TCCGCTTTGC GATCAATCTG CTGGCGAGCA CCCCGTCGAT 1441 TGTGTTTGGC ATCTTTGGTC TGAGCATTTT CATCGTTCTT CTGGGCTTGC CGTTTAGCAT 1501 TCTGGCGGCC GGCCTGACGA TGACCGTCGT GGTTCTGCCG ATGCTGATTA GCAACTTCGA 1561 GGACGCCCTG CAACAGGTCC CGCATCAATA CCGTGAGGCG GGCAGCGCAC TGGGTTTGAC 1621 CAAGATCCAG ACCCTGTTTA AGATTATTCT GCCAAATGCA ATGGAGGGCA TCATCACGGG 1681 TATTATTTTG GCCATGGCGA AAATCATCGG TGAAAGCGCG CCGATCTATC TGACGTTGGG 1741 CACCGATATC CAGATGCCGA CCCAGGGTTT CCTGTCCCAA GGTACCACCC TGACCACGGG 1801 CATTTACATG ATGGTTGCGG AAGGTATTCC GGGTCACGGC CAGGGTACGA TTTATCTGAT 1861 GGCATTGATC ACGATTATCC TGATCATTGG CCTGAACTTT ACCTCTGGTC GTCTGAGCAG 1921 CCTGCTGGTC CAAAAGAGCA AAGACCTGAA AGCAAAATCC AAAATTAAGC GTAAGGAATT 1981 TAATGTTAAA ACCAAGGCGA AAATCAAGAA GATGAAGCCA AGCCTGAAGT TTAAATGGAT 2041 GAAACTGCAG AGCAAATTTC GTAAAGGCGA AATCAAAATC ACGTTTTTTA AGGAGATGGC 2101 CTCCAAAAAT AAACTGTGGG TCAAACGTCG CAAAGAATAT CGCAAGCTGA AAAAGGGTGT 2161 GATTGACCAC GAGTGAAGAG CTTAGAGACC GCCTTCGCCT TTTCTAGGAT CGCTGAGGTG 2221 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC 2281 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC 2341 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC 2401 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 2461 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 2521 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 2581 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 2641 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 2701 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 2761 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 2821 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 2881 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 2941 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT 3001 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 3061 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT 3121 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT 3181 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC 3241 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG 3301 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC 3361 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG 3421 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA 3481 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA 3541 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT 3601 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG 3661 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA 3721 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA 3781 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT 3841 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT 3901 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA 3961 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC 4021 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 4081 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT 4141 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT 4201 GGAGTGGGAG //