LOCUS       Exported     3962 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3962
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2030..3928
                     /label="Mfl241"
                     /note=""
                     /translation="MTKIRPQDNFYDSVNKEWIDNNELPDGYASWGSFEMLQKKSTDDIKAIINELVNATKLDRDSKMIANLRSNYLNDKARNKQGIKPIQPILNKISALTDKKELTSLFVDLFQEWGISFFHSKGVDSDFKDSNLRALMIDSMGLGMSDRDFYEETHPRHEEIKNAYKNYIENLVKLSGVRLNTKDIFNLIYSFEEKISKSMFKQEELREPENIYNVVTIKELNDICPIVDWTEYLNKTGYDKASKIILTEPKYFEKLNQMIEEISLDDLKDIMSYKVTSSYSRMLTIDLYENSFKYGSVFSGVKKMKPIEDRVVEFVDGTLGELISKEYVKRHFSEDAKKDVLKMVNDLLKVYENRINTLDWMSDETKTKAIEKLNSFTIKIGYPDKWEDLSDVEILSYEEGGSLFDNMQSLAKHYIKKELKEINLPVDKTKWYMDAQTVNAYYNPTSNEICFPAGILQKPFYDVNQSHAANLGGIGAVIGHEVSHGFDDEGSKFDKDGNFENWWTETDNEQYKLRTQRVVDQYNEYQINGSNVNGKLTLGENIGDLSGVAAALDICKAQSPNDLKDFFTNYALVWRRKATDEQKNTRLLVDPHSPEEFRCNGVLVNINEFHEVYETKPGDGMYKPEEERTKVW"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATCAGTTT CTTTGGCAGC CGGTCTCAAA TGACGAAAAT
     2041 TCGCCCGCAG GACAACTTCT ATGATAGCGT CAACAAGGAA TGGATCGATA ACAATGAACT
     2101 GCCTGACGGT TACGCTAGCT GGGGTAGCTT CGAAATGCTG CAGAAAAAGT CCACCGACGA
     2161 TATCAAGGCG ATCATTAATG AGCTGGTGAA TGCTACGAAA CTGGATCGTG ATAGCAAAAT
     2221 GATCGCTAAT CTGCGCAGCA ATTATCTGAA TGACAAAGCC CGTAACAAAC AGGGTATTAA
     2281 GCCAATTCAA CCGATTCTGA ACAAAATCAG CGCTCTGACC GACAAGAAGG AACTGACCTC
     2341 CCTGTTCGTG GACCTGTTCC AGGAGTGGGG TATTTCGTTT TTCCATAGCA AAGGTGTGGA
     2401 CAGCGACTTC AAAGACAGCA ATCTGCGTGC ACTGATGATC GATAGCATGG GTCTGGGCAT
     2461 GAGCGACCGT GATTTCTATG AGGAAACCCA CCCGCGTCAC GAGGAGATTA AGAACGCGTA
     2521 TAAAAATTAC ATTGAAAACC TGGTGAAGCT GTCCGGTGTC CGCCTGAACA CCAAGGACAT
     2581 TTTTAATCTG ATCTATAGCT TTGAAGAAAA AATTAGCAAA AGCATGTTTA AACAAGAGGA
     2641 GCTGCGTGAA CCGGAAAACA TCTACAATGT TGTTACTATT AAGGAACTGA ATGATATCTG
     2701 CCCGATTGTG GATTGGACGG AGTACCTGAA CAAGACGGGC TATGATAAGG CGTCCAAAAT
     2761 CATTCTGACC GAACCAAAGT ACTTTGAAAA GCTGAATCAA ATGATTGAAG AAATTAGCCT
     2821 GGACGATCTG AAAGATATCA TGAGCTATAA GGTGACCAGC TCTTATTCTC GTATGCTGAC
     2881 GATTGACCTG TACGAGAACA GCTTTAAGTA CGGTAGCGTT TTCAGCGGTG TCAAGAAAAT
     2941 GAAGCCGATT GAGGACCGCG TTGTCGAGTT CGTTGACGGT ACCCTGGGCG AGTTGATTTC
     3001 TAAGGAGTAC GTGAAACGTC ACTTTAGCGA GGATGCAAAG AAGGACGTGC TGAAGATGGT
     3061 TAACGATCTG CTGAAAGTAT ACGAAAATCG CATCAACACC CTGGATTGGA TGAGCGACGA
     3121 AACCAAGACG AAGGCGATCG AGAAACTGAA TAGCTTTACC ATTAAGATCG GCTACCCGGA
     3181 CAAATGGGAA GATTTGTCCG ACGTGGAGAT TCTGAGCTAC GAGGAGGGTG GTAGCCTGTT
     3241 CGACAATATG CAAAGCCTGG CCAAACATTA CATTAAGAAG GAGCTGAAAG AGATTAATCT
     3301 GCCGGTCGAC AAGACCAAGT GGTATATGGA TGCGCAAACC GTCAACGCAT ACTACAACCC
     3361 TACGTCTAAT GAGATCTGTT TCCCGGCGGG TATTCTGCAA AAACCGTTCT ATGACGTTAA
     3421 CCAAAGCCAC GCGGCCAACC TGGGTGGTAT TGGTGCAGTG ATTGGTCACG AGGTGAGCCA
     3481 TGGTTTTGAC GACGAAGGTT CCAAATTCGA TAAGGACGGT AATTTCGAGA ACTGGTGGAC
     3541 CGAAACTGAT AACGAGCAAT ACAAATTGCG CACGCAGCGC GTGGTTGATC AGTACAATGA
     3601 GTACCAAATC AATGGTAGCA ATGTGAACGG CAAACTGACG TTGGGCGAAA ATATTGGCGA
     3661 TCTGTCCGGT GTGGCCGCAG CTCTGGATAT CTGCAAGGCT CAGAGCCCGA ATGACCTGAA
     3721 GGATTTCTTT ACCAACTATG CGCTGGTTTG GCGTCGTAAA GCCACCGACG AGCAGAAAAA
     3781 CACCAGATTG CTGGTGGACC CGCACAGCCC GGAAGAATTC CGCTGTAATG GTGTGCTGGT
     3841 TAACATTAAC GAATTTCATG AGGTCTATGA AACGAAACCG GGTGATGGTA TGTATAAGCC
     3901 GGAGGAAGAA CGCACCAAAG TTTGGTGAAG AGCTTAGAGA CCGTATGTCC CCTAAGCTCT
     3961 GG
//