LOCUS       Exported     4112 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4112
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4078
                     /label="Mfl246"
                     /note=""
                     /translation="MKKLLAILAAVGLTATTSSVVISCTTTVDRFGKLNFSDKQVYTSFILKMKDSGFISVSQAEKFLKISDTEIIADVLKILDKKIAEEEYKTTSSNIASTLKVKEKETTENITSNLLNDLATNKFFSEYTAKVISDKHGLVNDKQYSNNHSLNPFNLFEDDEKINYSIYYKEKSENETLTRWQVLGEFGENEWNIPSIEALNGGNNFYIIGSADNTKVTKLTDTKNTKVLNKDTKIDEVTGPFLVAKNEQEQGFSGNEIMKYRFQSYINAKIIPDLYTQLISLAYLDSNLYSTNLTTTNYTRSFVRLNTSNKLVSSVQNSLTSETKSSNVKLIWSFKAKVSTDATSWVKSYKQALGEPNSSGNIILDENSITTLKNNFSSNSLENNTKLGTDPFLGLVGYNGIAKNNDTGIEAISGSLSISTDAQTAAKSIDRPTLLTGPRDQGFAVGDNGESEIVLVLPIYLNDIYDNSNVTLNAVNQVATLSIPSDTWVPLGDKYSPYVDDMKTFTNETNVEIIKDNNGNLYVKALADKGSFTLGNQRTITVNVENANDTMLGIHETINKEDSAFTSGFDTEQQGKVKDTDKILYSVLWARNADPKSVYQLSEAWSNSVQSSSDIKNLSATNKQLLISEIENGLVAGDTDYTTEAKEELYTKYIMDGDNVLFQGLYDELAKYIKDEDGNTSD"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGCCCAG AAGGATACAA CGGTCTCAAA TGAAGAAGCT
     2041 GCTGGCGATT TTGGCCGCAG TTGGTCTGAC GGCGACGACC AGCTCCGTTG TAATCAGCTG
     2101 TACCACTACG GTTGACCGTT TTGGCAAGCT GAATTTCTCC GACAAACAGG TCTACACCTC
     2161 CTTCATTCTG AAAATGAAAG ACAGCGGTTT CATCTCCGTG AGCCAGGCAG AAAAGTTCCT
     2221 GAAGATTAGC GATACCGAGA TTATTGCTGA CGTTCTGAAA ATCCTGGACA AGAAGATCGC
     2281 AGAAGAGGAG TACAAGACCA CCAGCAGCAA CATTGCTAGC ACCCTGAAGG TTAAAGAAAA
     2341 GGAAACCACG GAGAACATTA CGTCTAATTT ATTGAACGAT CTGGCGACGA ATAAGTTTTT
     2401 CAGCGAGTAC ACTGCCAAAG TGATCAGCGA CAAGCATGGT TTGGTTAATG ATAAACAATA
     2461 CAGCAATAAC CATAGCCTGA ATCCTTTTAA TCTGTTCGAG GACGACGAGA AAATCAATTA
     2521 TAGCATTTAC TATAAAGAGA AAAGCGAGAA TGAAACCCTG ACCCGCTGGC AAGTGTTGGG
     2581 TGAATTTGGT GAAAACGAGT GGAACATTCC GAGCATCGAA GCGTTAAACG GTGGTAATAA
     2641 CTTCTATATT ATTGGTAGCG CCGACAATAC GAAAGTCACC AAACTGACCG ATACCAAGAA
     2701 TACGAAGGTG CTGAACAAAG ACACCAAAAT CGACGAAGTG ACTGGCCCGT TTCTGGTGGC
     2761 CAAGAATGAA CAAGAGCAGG GTTTTAGCGG TAATGAAATC ATGAAGTATC GTTTCCAGAG
     2821 CTATATCAAC GCGAAGATCA TTCCGGATCT GTACACCCAG CTGATCTCCC TGGCGTACCT
     2881 GGACAGCAAC CTGTATTCCA CGAACCTGAC CACGACTAAC TACACCAGAT CGTTCGTCCG
     2941 CCTGAATACC AGCAACAAAC TGGTCAGCTC CGTTCAGAAC AGCCTGACCA GCGAAACCAA
     3001 GAGCAGCAAC GTGAAACTGA TCTGGAGCTT CAAGGCCAAG GTTTCAACTG ATGCTACTAG
     3061 CTGGGTGAAA AGCTACAAAC AGGCTCTGGG CGAACCGAAC AGCAGCGGCA ACATTATCCT
     3121 GGATGAGAAT AGCATCACGA CGCTGAAGAA TAACTTCAGC AGCAATAGCC TGGAAAACAA
     3181 CACTAAGCTG GGTACCGACC CGTTTCTGGG CCTGGTTGGT TACAACGGTA TCGCGAAGAA
     3241 CAATGATACC GGTATCGAAG CGATCTCCGG CAGCCTGAGC ATCAGCACCG ACGCACAGAC
     3301 GGCGGCGAAA AGCATTGACC GCCCAACCCT GTTGACCGGT CCGCGTGATC AGGGCTTCGC
     3361 GGTGGGCGAT AATGGTGAGA GCGAAATCGT ACTGGTGCTG CCGATCTATC TGAACGACAT
     3421 CTACGACAAC AGCAACGTTA CCCTGAATGC TGTCAACCAA GTGGCGACGC TGTCGATTCC
     3481 GTCGGACACG TGGGTCCCAC TGGGTGACAA GTACAGCCCG TATGTTGACG ACATGAAAAC
     3541 CTTTACTAAT GAAACCAATG TCGAAATCAT TAAAGACAAC AATGGTAACC TGTATGTGAA
     3601 GGCGCTGGCA GACAAGGGCA GCTTCACCCT GGGCAACCAA CGTACCATTA CCGTGAATGT
     3661 CGAGAATGCA AACGACACCA TGCTGGGTAT TCATGAAACG ATTAATAAAG AGGATTCCGC
     3721 ATTCACCAGC GGTTTCGATA CCGAGCAGCA GGGTAAGGTT AAGGACACCG ATAAAATCCT
     3781 GTATAGCGTT CTTTGGGCCC GTAATGCCGA TCCAAAGAGC GTTTACCAGC TGAGCGAAGC
     3841 CTGGAGCAAT AGCGTTCAGA GCAGCAGCGA CATTAAAAAC CTGAGCGCCA CCAACAAGCA
     3901 GCTGCTGATC TCCGAGATTG AGAATGGTCT GGTAGCCGGT GACACCGATT ATACCACCGA
     3961 GGCCAAAGAG GAGCTGTACA CCAAGTATAT TATGGACGGT GACAATGTAT TGTTCCAAGG
     4021 TCTGTACGAT GAGCTGGCTA AGTACATTAA AGATGAGGAC GGCAATACCA GCGATTGAAG
     4081 AGCTTAGAGA CCGGTACGGT TTACAGACCA CT
//