LOCUS       Exported     3890 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3890
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3856
                     /translation="MIFLKLVKQTSYQDCGVACIAMMINHFYKYEIDLQQVKNYLSIHDDELSFYDVIDLASNFYLKGDAFKVEQDFLELKNKVPFLAQVINSDGLLHFVIVESILQNNLIVFDPSKEKKIKQNISEFLKCFNSNVIIFKSNIKSFNKDFKFNFKKFLNIFNFDFILYLLINLVSTLLFILDTQFLKMFSNSLSEQTQDFLIYLFPLIILLFNMLFKNISINILNKNYKKRKHFLLKKFLNLIESTNSEDLFYLYQEIKWVCQYENYSLKSSISSFISEVVSLFFIYFIIKELFLIILIADVIYILINTFINNFVKKDSFNQDKEWFTFLSNIEHVKNICAEYDMLTELLKLEEKDKLASSTLNWIELWDKVGLILIYIVSWSLLKNGNLEFSLFFIILLFKNFSRINIFNLGQTLIWIKKYKISIIKFEKIFIEKNEVNFNEEISNILISNKQQKLELNKGLNIINFKIDLGNVNKTKNDIEVDVYINNKNISILKTSDLRKQIYYSKNFQIKFGTIFQNITSSNINSINIFNIKEINELLNKYSIKITKLVKPETNSQIEKEIIKLLNVFYINKKVILIKNDFQILTFDEIKSILTIFTELSDDKFLILS"
                     /label="Mfl264"
                     /note=""
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATCAGTTT CTTTGGCAGC CGGTCTCAAA TGATTTTTCT
     2041 GAAGCTGGTT AAACAAACCA GCTACCAAGA TTGTGGTGTG GCTTGTATCG CCATGATGAT
     2101 CAACCACTTT TATAAATACG AGATTGATTT GCAACAGGTT AAGAACTATC TGTCCATTCA
     2161 TGACGACGAA CTGAGCTTCT ACGATGTCAT TGATTTAGCG TCTAACTTTT ACCTGAAGGG
     2221 TGATGCGTTC AAAGTTGAGC AAGATTTCCT GGAGCTGAAG AATAAGGTTC CATTCCTGGC
     2281 TCAGGTGATC AATTCCGATG GCTTGCTGCA CTTCGTTATC GTCGAAAGCA TCCTGCAGAA
     2341 CAACTTGATT GTATTTGACC CTAGCAAGGA GAAGAAAATC AAACAAAACA TTAGCGAGTT
     2401 TCTGAAGTGC TTCAACAGCA ATGTTATTAT TTTCAAAAGC AACATTAAAT CCTTCAACAA
     2461 GGACTTCAAG TTCAATTTTA AAAAATTCTT AAATATCTTT AACTTCGACT TCATTCTGTA
     2521 TCTGCTGATT AACCTGGTTA GCACGCTGTT GTTCATCCTG GATACCCAGT TTCTGAAGAT
     2581 GTTTAGCAAT TCGTTATCAG AGCAGACCCA GGACTTCCTG ATTTACCTGT TCCCGCTGAT
     2641 CATCCTGCTG TTTAACATGT TGTTCAAGAA TATCAGCATT AATATTCTGA ATAAAAATTA
     2701 CAAGAAACGC AAACACTTTC TGCTGAAGAA GTTTCTGAAT TTGATTGAGA GCACGAACAG
     2761 CGAGGATTTG TTTTATCTCT ACCAGGAAAT TAAATGGGTA TGTCAGTATG AGAATTACTC
     2821 GCTGAAGTCC AGCATCAGCT CCTTCATCAG CGAGGTGGTC AGCTTGTTTT TCATTTACTT
     2881 TATCATCAAA GAACTGTTCT TGATCATCCT GATCGCCGAT GTTATTTATA TTCTGATTAA
     2941 TACGTTTATT AACAATTTCG TGAAGAAAGA TTCGTTCAAT CAGGACAAGG AATGGTTTAC
     3001 CTTCCTGAGC AACATTGAAC ACGTCAAAAA CATTTGCGCG GAATATGACA TGCTGACCGA
     3061 GCTGCTGAAA CTGGAAGAGA AAGACAAACT GGCAAGCAGC ACGTTAAACT GGATTGAACT
     3121 GTGGGACAAG GTGGGCCTGA TCCTGATTTA CATCGTGAGC TGGAGCCTGC TGAAAAACGG
     3181 TAATCTGGAA TTTAGCCTGT TTTTCATCAT TCTGCTGTTC AAGAACTTTA GCCGCATCAA
     3241 CATTTTCAAC CTGGGCCAAA CCTTGATCTG GATTAAAAAA TATAAAATCA GCATCATTAA
     3301 ATTCGAAAAA ATTTTCATCG AGAAGAACGA GGTGAACTTC AACGAGGAGA TTAGCAACAT
     3361 TCTGATCTCA AACAAGCAAC AGAAGCTGGA GCTGAATAAG GGCCTGAATA TCATCAACTT
     3421 CAAGATCGAT CTGGGCAACG TCAATAAGAC GAAGAATGAT ATTGAAGTGG ACGTCTACAT
     3481 CAATAACAAG AATATCTCTA TCCTGAAAAC CAGCGATCTG CGTAAGCAAA TTTACTACTC
     3541 TAAAAATTTC CAGATCAAAT TCGGTACCAT CTTCCAAAAC ATCACCAGCT CGAATATCAA
     3601 CAGCATTAAT ATTTTCAATA TTAAGGAGAT TAACGAACTG CTGAACAAGT ACAGCATCAA
     3661 GATCACCAAA TTGGTGAAGC CGGAAACTAA CAGCCAAATC GAAAAGGAAA TCATTAAGCT
     3721 GTTGAATGTG TTTTATATCA ATAAGAAAGT GATCCTGATC AAGAATGATT TCCAAATTCT
     3781 GACCTTCGAT GAAATCAAAA GCATCTTGAC TATCTTTACC GAGCTGTCCG ATGATAAGTT
     3841 TCTGATCCTG TCCTGAAGAG CTTAGAGACC CGAGTGTAGT TCCCGTCAAT
//