LOCUS Exported 2969 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2969 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..2935 /label="era" /note="An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism." /translation="MNKIKSGFISIVGRPNVGKSTLLNKIIGHKISIVTNKAQTTRNNIRGILTEKEYQLIFVDTPGIHTSKNQIDRFMNSSAMRSMKEVDVVVFMAPADETIGKNDLFILNELSKKNDIKKILVISKADVVSKEKLFLKATEWNTYEQIFDEIIITSSTENINIDKLIETIVGFLPETGHYFYDEESITDQPNRFAIREIIRESVLLKAGQEVPHSVAILVDELEETEDEINIVASIIVERKSQKGIIIGHQGKKISDIKYKSRKQIRELFEKDVNLELFVKVQENWRNSASLIKKMGYDKDKY" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAGGATATG TAGACACCGC CGGTCTCAAA TGAATAAAAT 2041 CAAAAGCGGC TTCATTAGCA TTGTCGGTCG CCCGAATGTG GGTAAGAGCA CTCTGCTGAA 2101 CAAAATTATC GGCCATAAGA TTTCAATTGT CACGAACAAA GCTCAAACGA CGCGTAACAA 2161 CATTCGTGGC ATTCTGACCG AGAAAGAGTA CCAGCTGATT TTTGTTGACA CGCCGGGTAT 2221 TCACACCAGC AAAAACCAGA TTGACCGTTT CATGAATAGC AGCGCAATGC GCAGCATGAA 2281 AGAGGTGGAT GTGGTCGTTT TCATGGCTCC GGCGGACGAA ACGATTGGTA AGAACGATTT 2341 GTTCATCCTG AACGAGCTGA GCAAGAAGAA TGATATTAAG AAGATCCTGG TTATCAGCAA 2401 AGCAGACGTG GTTTCGAAGG AAAAGCTGTT TCTGAAAGCT ACGGAGTGGA ACACCTACGA 2461 ACAAATCTTT GACGAGATCA TCATTACGAG CAGCACCGAA AACATCAACA TCGACAAACT 2521 GATTGAAACG ATTGTCGGTT TTCTGCCAGA AACCGGCCAC TACTTTTATG ATGAAGAAAG 2581 CATCACGGAC CAACCTAATC GTTTCGCGAT CCGTGAGATT ATTCGCGAGA GCGTTCTGTT 2641 GAAGGCAGGC CAGGAAGTCC CGCACAGCGT TGCAATCCTG GTGGACGAGC TGGAGGAAAC 2701 GGAGGACGAA ATCAACATCG TGGCTAGCAT TATCGTTGAG CGTAAAAGCC AGAAAGGTAT 2761 CATTATCGGC CACCAAGGTA AGAAGATCAG CGATATTAAG TACAAGTCTC GTAAACAGAT 2821 TCGTGAACTT TTCGAAAAGG ATGTCAACCT GGAGTTGTTC GTAAAAGTCC AGGAAAACTG 2881 GCGTAACAGC GCCAGCCTGA TCAAGAAAAT GGGCTACGAT AAGGATAAGT ACTGAAGAGC 2941 TTAGAGACCG AGGTCAATCA TGCCTCTCT //