LOCUS       Exported     3418 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3418
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1456..1503
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3351..3367
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3368..3399
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1384
                     /note="Catalyzes the attachment of glycine to tRNA(Gly)."
                     /translation="MEKLIAHLKSQGFIFQGSEIYGGLANSWDYGPLGVEVKNKLKQAWWNHFVRKNPYNIGLDSSIILNSSVWKASGHIDGFNDPLIDCKKCNSRWRADKLIEEFNSEINAGVMTENQMEEFIREQNIKCPKCQACDFTQIRKFALMFKTNQGVLEDESSSVYLRPETAQGIFINFKNAQRSLRKKLPFGIGQIGKSFRNEITPGNFIFRTREFEQMELEFFFNPSDEKDWFSYWLNEVETFLQDKIQINKENYRVRSHEKDELAHYSTATSDIEFKFPFGWGELWGVAHRGNFDLNAHQEASKQDLTYLDPTTNQKVLPHVIEPSVGVERMMLAILWQAYHEEDLGEGNSRIVMKLPYNLAPYQIAVMPLQKQQNDQAQALYSNLLNNFDVTYDETGNVGKRYRRQDAIGTPFVITVDFDTPETNSVTVRERDSMEQVRINLDELEAYLKAKF"
                     /label="Mfl268"
     primer_bind     complement(1508..1524)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1591..2179)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2370..3230)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3231..3335)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCAAAT GGAGAAATTA ATTGCACATC TCAAGAGCCA
       61 GGGCTTCATT TTCCAAGGTA GCGAAATTTA TGGTGGTCTG GCGAATAGCT GGGACTATGG
      121 CCCGCTGGGT GTTGAGGTGA AAAACAAATT GAAGCAAGCG TGGTGGAACC ACTTCGTGCG
      181 TAAGAACCCA TATAACATCG GTCTGGATAG CAGCATTATC CTGAATAGCA GCGTGTGGAA
      241 AGCGAGCGGC CATATTGACG GCTTTAATGA TCCGTTGATT GACTGTAAGA AATGCAACTC
      301 CCGTTGGCGT GCAGACAAGC TGATCGAGGA GTTCAATTCC GAAATTAATG CGGGTGTCAT
      361 GACCGAAAAC CAGATGGAAG AATTCATCCG TGAACAGAAT ATTAAATGCC CAAAATGCCA
      421 GGCATGTGAT TTCACCCAAA TTCGTAAGTT TGCGCTGATG TTTAAAACCA ACCAGGGCGT
      481 GCTGGAGGAT GAGAGCAGCA GCGTCTATCT GCGTCCAGAA ACCGCCCAAG GTATCTTCAT
      541 TAATTTTAAA AATGCACAGC GTTCCCTGCG CAAGAAGCTG CCGTTCGGTA TTGGCCAAAT
      601 CGGTAAGAGC TTCCGCAACG AAATTACGCC GGGTAACTTC ATCTTCCGCA CGCGCGAGTT
      661 TGAGCAGATG GAGCTCGAGT TCTTTTTTAA CCCGTCTGAT GAGAAAGATT GGTTCTCTTA
      721 TTGGCTGAAT GAAGTTGAAA CCTTTCTGCA AGATAAAATC CAAATTAACA AGGAGAATTA
      781 CCGCGTCCGT AGCCATGAGA AAGACGAGCT GGCGCACTAT AGCACCGCTA CCAGCGACAT
      841 TGAGTTTAAG TTCCCGTTTG GCTGGGGTGA GCTGTGGGGT GTTGCTCACC GCGGCAATTT
      901 TGACTTAAAC GCGCATCAGG AGGCTTCCAA GCAGGACTTG ACCTACCTGG ATCCGACGAC
      961 GAACCAAAAA GTTCTGCCGC ATGTCATTGA GCCATCCGTT GGTGTGGAGC GTATGATGCT
     1021 GGCTATCCTG TGGCAAGCGT ATCATGAAGA GGACTTGGGT GAGGGTAACT CTAGAATCGT
     1081 TATGAAGCTG CCGTACAACC TGGCCCCGTA TCAGATCGCG GTCATGCCGC TGCAGAAGCA
     1141 GCAGAACGAC CAGGCCCAAG CGCTCTACTC GAATCTGCTG AATAATTTCG ACGTTACCTA
     1201 CGACGAAACC GGCAACGTGG GTAAGCGTTA CCGTCGCCAG GACGCTATTG GTACCCCGTT
     1261 TGTTATTACG GTGGACTTCG ATACGCCGGA AACCAACTCC GTCACTGTGC GTGAGAGAGA
     1321 CAGCATGGAA CAAGTCCGTA TTAACCTGGA CGAGCTGGAG GCCTACCTGA AGGCGAAATT
     1381 TTGAAGAGCT TAGAGACCGG CATCGGACCC TTATAACTCG CTGAGGTGTC AATCGTCGGA
     1441 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1501 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1561 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1621 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1681 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1741 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1801 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1861 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1921 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1981 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     2041 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     2101 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     2161 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     2221 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     2281 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     2341 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     2401 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     2461 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2521 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2581 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2641 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2701 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2761 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2821 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2881 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2941 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     3001 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     3061 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     3121 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     3181 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     3241 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     3301 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     3361 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG
//