LOCUS       Exported     3478 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3478
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1516..1563
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3411..3427
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3428..3459
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1444
                     /translation="MKKYMDKKEIAKIKTIEDFYEATVEFAKQNNNEITSEEVQMSFSKIFANATDNEYEKLLEDLQAKGIQFTDLEDIDIDEEIDLDEEVDEDAEISDDDAYADELIGERKGPGRRPKDAGTTKYRVGSISNETKIQDLIKTYFSTIGQTKILTKDQEIVYAKLANSEDPEERKEGRDMLITSNLKLVISVARKHLNRGLDFADLIEEGNIGLIKAVDKFDYEKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLSRIERQLTQELGREPSSKEVAERMGGDMTAEKVVEIKKIAVEPVSLEKPFGDEDDTHFGDFVEDKDMISPTDFTEKEILREVIDKVFEDMPAREEKVIRMRYGIVPTKVRTLIRLAEECNDETAGELAKAINKLDIHLETPVEKIRTVDSKIIQDHLLKYEASKTLEEVGKELNVTRERIRQIEAKTIRKLKQPANANKSGKVLKEFYKG"
                     /label="Mfl270"
                     /note="Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released."
     primer_bind     complement(1568..1584)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1651..2239)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2430..3290)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3291..3395)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAACGAGCA GACCGAATAG GGTCTCAAAT GAAAAAGTAC ATGGATAAGA AAGAAATCGC
       61 GAAGATCAAG ACGATTGAGG ACTTTTATGA AGCCACCGTT GAGTTCGCTA AGCAGAATAA
      121 TAACGAGATC ACCAGCGAAG AGGTTCAGAT GAGCTTCTCT AAGATTTTTG CCAATGCAAC
      181 CGACAATGAA TACGAAAAGC TCCTGGAGGA CTTGCAGGCA AAGGGTATCC AGTTCACGGA
      241 CCTGGAGGAC ATCGACATCG ACGAAGAGAT TGATCTGGAC GAAGAAGTGG ACGAGGACGC
      301 GGAGATTTCC GACGACGACG CATATGCGGA CGAGCTGATT GGTGAGCGCA AAGGTCCGGG
      361 TCGTCGCCCG AAAGATGCGG GTACTACCAA ATACCGCGTC GGCAGCATTA GCAATGAAAC
      421 CAAAATCCAA GATTTGATCA AGACGTACTT CAGCACCATC GGCCAAACCA AGATTCTGAC
      481 GAAAGACCAG GAAATTGTGT ACGCTAAGCT GGCGAATAGC GAGGACCCGG AGGAGCGTAA
      541 GGAAGGCCGT GACATGCTGA TTACGTCCAA CCTGAAGCTG GTTATCAGCG TTGCGCGTAA
      601 ACACCTGAAT CGTGGTCTGG ATTTTGCGGA CCTGATTGAA GAGGGTAATA TCGGCCTCAT
      661 CAAGGCTGTC GACAAGTTCG ATTACGAAAA AGGCTTCAAA TTTTCCACCT ACGCTACCTG
      721 GTGGATCAGA CAGGCTATTA CCCGTGCTAT CGCGGACCAG GCGCGTACTA TCCGTATTCC
      781 TGTGCACATG GTGGAAACCA TTAACAAGCT CAGCCGCATT GAGCGTCAAC TGACGCAGGA
      841 GCTGGGCCGT GAGCCGAGCT CCAAAGAGGT TGCAGAACGT ATGGGCGGCG ACATGACGGC
      901 GGAAAAGGTG GTGGAGATCA AGAAAATCGC GGTCGAACCG GTGTCACTGG AGAAACCGTT
      961 CGGTGACGAG GATGATACCC ATTTCGGCGA CTTCGTGGAG GACAAGGATA TGATTAGCCC
     1021 GACGGATTTC ACTGAGAAAG AAATCCTGCG TGAGGTTATC GACAAGGTCT TTGAGGACAT
     1081 GCCTGCGCGT GAAGAGAAAG TCATTCGTAT GCGCTACGGT ATTGTTCCAA CCAAAGTACG
     1141 TACGCTGATC CGTCTGGCGG AGGAGTGCAA TGATGAAACC GCGGGCGAGC TGGCCAAAGC
     1201 CATCAACAAG CTGGATATTC ACTTAGAAAC CCCGGTCGAA AAAATTCGCA CGGTGGATAG
     1261 CAAGATCATC CAAGACCATC TGCTGAAGTA TGAAGCATCC AAAACCCTGG AAGAGGTTGG
     1321 CAAGGAGCTG AATGTGACCC GCGAACGTAT TCGCCAGATC GAGGCAAAAA CGATCCGCAA
     1381 ACTGAAGCAA CCGGCCAATG CGAACAAAAG CGGTAAGGTG CTGAAGGAGT TCTACAAGGG
     1441 CTGAAGAGCT TAGAGACCGG GAATCTCGTC GGAAATCTCG CTGAGGTGTC AATCGTCGGA
     1501 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1561 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1621 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1681 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1741 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1801 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1861 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1921 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1981 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     2041 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     2101 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     2161 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     2221 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     2281 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     2341 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     2401 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     2461 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     2521 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     2581 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     2641 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     2701 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2761 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2821 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2881 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2941 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     3001 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     3061 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     3121 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     3181 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     3241 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     3301 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     3361 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     3421 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG
//