LOCUS       Exported     3607 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3607
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1645..1692
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3540..3556
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3557..3588
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1573
                     /label="Mfl274"
                     /note=""
                     /translation="MARKKRIKIYSSNGKKALLLSPEEITIVKNDPSYEKNYEIEFNKGKQAFEVRNYYAIGFWKDLLFVLIGAFLSTIAIDYFISITGNAGLFPGGLGAIARFLSIIGANNITISASTLYFIVYFIMNIPLVIFGFIKIGWKFSTLTLIYSIISIFFDLILQNIPYINPNEFSLLIDYNLISSLPGAWGAIIWIFAFAIFGGMINGFSYSITYKGNASTGGSDWVTYYYSKKLNKDIGSLNIKINIFILFIVICLNTIILKTQHIDQTIKLSAIYNEYNSWNELINSDLGKALDPILSAEDKVSIDLFNSWKNLKSGNWNNEDWFNISKYISSSSEYDSYYTSTLVWKMKFKWIIGPSLFASLTLIITQGLTVNRLYPKNKILNLFISTNKINEVQQYLFSVGYTNNIFIWRTVASKKNAWNDQEQDLIMISMPLLYFNKIEDYLIKIDEDMIINIIGSKAVKGKNFSYTFDNELREKALTEEFMNNGKLMKKIESNTIIKTHKKMKKNGVSIQNEV"
     primer_bind     complement(1697..1713)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1780..2368)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2559..3419)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3420..3524)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GGCACGTAAG AAGCGTATCA AAATTTACAG
       61 CTCTAACGGT AAAAAGGCCC TGCTGCTGTC GCCGGAGGAA ATCACCATTG TTAAGAATGA
      121 CCCGAGCTAT GAAAAGAATT ACGAGATTGA GTTCAACAAA GGTAAACAAG CGTTCGAGGT
      181 TCGTAATTAC TACGCGATTG GTTTTTGGAA GGATCTGCTG TTCGTGCTGA TCGGCGCATT
      241 TCTGAGCACC ATTGCTATCG ATTACTTCAT TTCTATCACC GGTAATGCGG GTCTGTTCCC
      301 GGGTGGTCTG GGTGCAATTG CACGCTTTCT GAGCATTATC GGCGCAAACA ATATTACGAT
      361 CTCCGCAAGC ACCCTGTACT TCATCGTCTA CTTCATCATG AATATTCCGC TGGTGATTTT
      421 TGGCTTCATT AAGATCGGTT GGAAATTTAG CACGCTGACC CTGATCTATT CCATTATTAG
      481 CATTTTCTTT GACCTGATCC TGCAAAATAT CCCGTACATT AACCCTAATG AGTTCAGCCT
      541 GCTGATCGAT TATAATTTGA TCTCTAGCCT CCCGGGCGCG TGGGGTGCCA TTATCTGGAT
      601 CTTCGCGTTT GCCATCTTTG GTGGCATGAT CAATGGTTTT AGCTATTCTA TTACGTATAA
      661 AGGCAATGCG AGCACCGGCG GCTCTGACTG GGTGACGTAC TATTACAGCA AAAAGCTGAA
      721 TAAGGATATC GGTAGCCTGA ATATCAAAAT TAATATCTTC ATCCTGTTTA TTGTTATCTG
      781 CTTGAACACC ATTATCTTAA AGACCCAACA CATCGATCAA ACCATCAAAC TGAGCGCCAT
      841 TTATAACGAA TACAATTCGT GGAATGAGCT GATTAATAGC GATCTGGGCA AAGCGCTGGA
      901 TCCGATCCTG TCGGCAGAAG ATAAGGTTAG CATCGACCTG TTCAACAGCT GGAAGAACCT
      961 GAAAAGCGGC AACTGGAACA ACGAAGATTG GTTTAATATT AGCAAATATA TCAGCAGCAG
     1021 CTCCGAATAC GATAGCTATT ATACGAGCAC GCTGGTGTGG AAAATGAAGT TCAAATGGAT
     1081 TATTGGTCCG AGCCTGTTTG CGAGCTTGAC GCTGATTATT ACGCAGGGCC TGACCGTGAA
     1141 CCGTCTGTAT CCGAAAAACA AAATCCTGAA TCTGTTCATC TCTACGAATA AAATCAATGA
     1201 AGTCCAACAG TACCTGTTTA GCGTCGGTTA CACGAATAAT ATCTTTATCT GGCGCACCGT
     1261 CGCGTCCAAG AAGAATGCGT GGAACGATCA GGAGCAAGAC CTGATCATGA TCAGCATGCC
     1321 TTTGCTGTAC TTCAATAAAA TCGAGGACTA TCTGATTAAA ATCGACGAGG ACATGATTAT
     1381 CAATATCATT GGTAGCAAAG CTGTTAAAGG CAAAAACTTC AGCTATACCT TCGATAATGA
     1441 GCTGCGTGAG AAAGCTTTGA CGGAAGAGTT CATGAATAAC GGTAAGCTGA TGAAGAAGAT
     1501 TGAAAGCAAT ACGATCATTA AAACGCATAA AAAGATGAAG AAGAATGGTG TAAGCATCCA
     1561 GAACGAAGTG TGAAGAGCTT AGAGACCCGT GAATCTTGAC CTGTGGTCGC TGAGGTGTCA
     1621 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     1681 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     1741 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     1801 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1861 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1921 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1981 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     2041 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     2101 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     2161 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     2221 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     2281 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     2341 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG
     2401 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     2461 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     2521 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     2581 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     2641 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     2701 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     2761 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     2821 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     2881 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     2941 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     3001 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     3061 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     3121 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     3181 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     3241 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     3301 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     3361 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     3421 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     3481 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG
     3541 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA
     3601 GTGGGAG
//