LOCUS       Exported     6074 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..6074
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..6040
                     /label="Mfl275"
                     /note=""
                     /translation="MNNKKASFKKIAQSLCLVLIVFSLIISIVFSSFSIANKTNLNTRYSGGYDALVEVYDEKQNATQGTSTPNGDARLAAESLQTKLSPFSDNTIDVKVVGQHRVSIRATKEQYQNNPRLFINAIEQDGGLMAFTQTNNIYTDILFDDTAVKKITASENGIYDSNNEIANKLAISEIFGSVKYTPDRSESGSGQQNSPFLTFEEGSNGSYLKNLTAATTATENAPATPANLTVISSFETILNNLREYFLQAPNENSLDTYLENYYRGVIQPILSFYSSTDTTAQQRAVIDDFFSVSYMTSSGQMQSASLISGSFNKWWTDSASNEQMLFNNSNNIELIVNDLKALLYGTFEGRTAPTNYTYNFVNNVNKYVIDPNSKTEDFKKEPENGNPAGRYSENIILGAQSMRIDEVAKVINDVMLSKVLFAQKSSTSIFSQYLDQSIFEKNFIMINDSVTQTGVASASETMSRATFTPSVVYNGATSQLRVKTRSATIARTIEASISQTTSGFSFKVIKLTEFNPEITLYMLLASIIFLLILAIFTMVFLVIAYRLLGIYTLIIAVTSVFITLFTPTLFGIAIGIELYTLIFIMLGLVLESCILTIEAFKKHLNKEKRSITESFKLSYKENLGIILDSFILILIPNLIIFWIGTGSLKNFATVATVSTAIILFLVIVVFRLMIYLTVKIQIFKNHPLWLPIDTKDIKSGVAIRDKINLSKYEYQLNILTNKEKVSSKELLKIKRINDLIETLKIKIENKEKDYNQKLINKNKEKTIKLNQKIESLEKTEKKIRWYKKDWISFLKVNRDVAKAVSTNAEGKVIEEVKTKRNQNWIFNINRIIIVLLLVFSVIGGVVAATVGPNYSNLFGKDNTYIVYGEYLYELPGNNFDNVVTKIAENQSEEQANAFQSEALRMANEKGHSDIEAGANDNEFVAWLSEYTVQYVFDNNLLKAFYSGYKGTTSLNSIESGTTYGDQTENSSAENLMPYIQIELSNQTNITRTKRLLNSIFTGRTRLGSNYTNESRGILGMYQIPFTAYGQINQILISFAILILILIVYILIRYKWTYYVALALALVVVVLITTSLVIMFRVPISTEILVTMISIAAFTIMSVIFILGKTKSLISIKNKNELRFEFDKEANAQAIIKHKVLEAINNKKALNKANKKRIKDIRRKIVDIKIAKKSKFYTWDAFKAIFKSNRIPKIAELRKEIKAIRKENKTDLKPLRKAIKAAKKAGRKEISTILKDNQFAKSLFVGSLRFGINRLVYISGFYLLFAIILAVTIPSLAGVGITLLIGVFVANIVILTMSLPLLIWLEKRRIVSNYGRKEFISQTNVSQEEQIVKDIND"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCAAA TGAACAACAA
     2041 AAAAGCATCG TTTAAGAAAA TCGCACAATC GCTGTGTTTG GTTCTGATCG TTTTCAGCCT
     2101 GATTATCTCC ATTGTTTTCT CCAGCTTTTC TATCGCTAAT AAGACGAACC TGAACACCCG
     2161 TTATAGCGGC GGTTACGATG CGCTGGTTGA AGTTTATGAC GAAAAACAAA ACGCGACCCA
     2221 AGGTACCTCT ACCCCGAACG GTGATGCGCG TCTGGCCGCT GAGAGCTTGC AGACGAAGCT
     2281 GTCGCCGTTC AGCGACAACA CGATCGATGT TAAGGTTGTC GGTCAGCACC GCGTTTCCAT
     2341 TCGCGCAACC AAGGAGCAAT ATCAGAACAA TCCGCGTCTG TTCATTAATG CCATCGAACA
     2401 GGATGGCGGT CTGATGGCGT TCACCCAGAC CAACAATATT TACACCGATA TTCTGTTTGA
     2461 CGACACTGCA GTTAAGAAGA TTACCGCGAG CGAAAATGGC ATCTACGATT CTAACAACGA
     2521 AATCGCGAAT AAATTGGCCA TTAGCGAGAT TTTTGGTTCG GTGAAGTACA CGCCGGATCG
     2581 CAGCGAATCG GGTAGCGGCC AGCAAAACTC TCCGTTCCTG ACGTTTGAAG AAGGTAGCAA
     2641 TGGTTCCTAT CTGAAAAATC TCACCGCAGC GACGACTGCA ACCGAAAATG CTCCGGCGAC
     2701 CCCGGCGAAT CTGACGGTCA TCAGCAGCTT TGAAACGATC CTGAATAATC TGCGTGAGTA
     2761 TTTCCTGCAG GCCCCGAATG AAAACAGCTT GGACACCTAC CTGGAAAATT ACTATCGCGG
     2821 CGTCATTCAG CCGATCCTGA GCTTTTACAG CAGCACCGAC ACCACCGCCC AGCAACGTGC
     2881 GGTTATTGAT GATTTTTTCA GCGTTAGCTA TATGACCAGC TCCGGTCAAA TGCAGAGCGC
     2941 GTCTTTAATT AGCGGTTCTT TTAACAAATG GTGGACGGAT AGCGCTTCTA ACGAGCAAAT
     3001 GCTGTTTAAT AACTCAAACA ATATCGAGTT GATTGTCAAC GACTTGAAGG CATTGCTGTA
     3061 CGGTACGTTT GAGGGTCGCA CCGCCCCGAC TAACTACACC TATAACTTTG TCAACAATGT
     3121 GAACAAATAC GTTATTGATC CAAATTCGAA AACCGAAGAT TTTAAAAAAG AACCGGAAAA
     3181 CGGTAATCCG GCGGGTCGTT ACAGCGAGAA CATTATCCTG GGTGCGCAAA GCATGCGCAT
     3241 CGACGAGGTG GCTAAAGTGA TCAACGACGT CATGCTGTCC AAGGTTCTGT TTGCTCAGAA
     3301 GTCGTCGACG TCCATCTTTA GCCAGTACCT GGACCAGAGC ATCTTCGAAA AGAACTTTAT
     3361 TATGATTAAT GACTCTGTGA CCCAGACCGG TGTCGCTAGC GCAAGCGAAA CCATGAGCCG
     3421 TGCCACGTTT ACCCCGAGCG TTGTTTATAA CGGCGCGACC TCCCAACTGC GTGTCAAGAC
     3481 CCGTAGCGCG ACTATTGCCC GCACGATTGA GGCGAGCATT AGCCAGACGA CTAGCGGTTT
     3541 TAGCTTCAAA GTTATCAAAC TGACCGAGTT TAACCCGGAA ATTACCCTGT ACATGCTGTT
     3601 GGCGAGCATT ATTTTTCTGC TTATCCTGGC AATTTTTACC ATGGTGTTTC TGGTGATCGC
     3661 TTACCGTCTG TTGGGTATTT ACACCCTTAT TATCGCGGTT ACCAGCGTGT TTATTACCCT
     3721 GTTTACCCCG ACGCTGTTTG GTATCGCAAT TGGTATTGAA CTGTACACTC TGATTTTCAT
     3781 TATGTTGGGC CTGGTTCTGG AGAGCTGCAT TCTTACGATT GAGGCCTTTA AGAAGCATCT
     3841 GAACAAAGAA AAACGTAGCA TTACCGAATC CTTCAAGCTG TCTTATAAAG AAAACCTGGG
     3901 TATCATTCTG GATAGCTTCA TCCTGATTCT GATTCCGAAT CTGATCATTT TTTGGATTGG
     3961 TACCGGCTCG CTGAAGAACT TTGCGACGGT TGCGACGGTC AGCACCGCGA TTATCCTGTT
     4021 TCTGGTAATT GTTGTTTTCC GCCTGATGAT CTACTTGACG GTTAAGATTC AGATCTTTAA
     4081 GAACCACCCG CTGTGGCTGC CGATTGATAC CAAGGACATC AAAAGCGGCG TTGCGATCCG
     4141 TGACAAAATT AATCTGAGCA AGTATGAGTA TCAGCTGAAC ATTCTGACCA ATAAGGAGAA
     4201 AGTTAGCAGC AAAGAATTGC TGAAGATCAA ACGCATCAAT GATTTGATCG AAACCCTGAA
     4261 AATCAAGATC GAAAACAAGG AGAAAGATTA TAACCAGAAA CTGATTAACA AAAATAAGGA
     4321 AAAAACGATT AAATTGAACC AAAAGATCGA ATCTCTGGAA AAAACGGAAA AGAAAATTCG
     4381 CTGGTATAAA AAAGACTGGA TTTCCTTCCT GAAGGTCAAC CGTGACGTGG CCAAAGCGGT
     4441 GTCCACCAAT GCCGAGGGCA AGGTTATTGA AGAAGTGAAA ACTAAGCGCA ACCAAAACTG
     4501 GATCTTCAAC ATCAATCGTA TCATTATCGT GCTCTTGCTT GTGTTTAGCG TTATCGGCGG
     4561 TGTCGTTGCC GCGACCGTGG GCCCGAACTA CAGCAATCTG TTCGGTAAAG ATAACACCTA
     4621 CATCGTGTAC GGCGAGTATC TGTATGAGCT GCCGGGTAAC AATTTTGATA ATGTGGTAAC
     4681 GAAGATTGCA GAGAACCAGA GCGAGGAGCA GGCGAATGCG TTCCAATCCG AAGCGTTGCG
     4741 CATGGCAAAC GAGAAGGGTC ATTCCGACAT CGAGGCGGGT GCAAACGACA ATGAGTTTGT
     4801 TGCATGGCTG AGCGAGTATA CCGTTCAGTA TGTGTTTGAC AACAATTTGC TGAAGGCCTT
     4861 TTACAGCGGT TACAAGGGCA CCACCAGCTT GAATAGCATC GAGTCTGGTA CTACCTACGG
     4921 TGACCAGACG GAAAACTCGA GCGCGGAGAA CCTGATGCCT TATATCCAAA TTGAACTGAG
     4981 CAATCAGACC AACATCACGC GTACGAAGCG CTTACTGAAC AGCATCTTCA CCGGCCGTAC
     5041 CCGTTTGGGC AGCAACTACA CCAATGAGTC CCGCGGCATC TTGGGTATGT ATCAAATCCC
     5101 GTTTACGGCC TACGGTCAAA TCAACCAAAT CCTGATCTCC TTTGCCATTC TGATCCTGAT
     5161 TCTGATCGTT TATATCCTGA TCCGTTACAA ATGGACGTAT TATGTGGCTC TGGCCCTGGC
     5221 GCTGGTTGTT GTTGTGCTGA TTACTACGAG CTTGGTTATT ATGTTCCGCG TACCGATTAG
     5281 CACGGAAATC CTTGTTACTA TGATTTCAAT CGCAGCTTTC ACTATTATGA GCGTGATTTT
     5341 CATTTTGGGC AAGACCAAAA GCCTGATTAG CATTAAGAAC AAAAACGAGC TGCGCTTTGA
     5401 ATTTGACAAG GAGGCCAACG CGCAAGCAAT CATTAAACAT AAGGTTCTGG AAGCCATTAA
     5461 CAATAAGAAG GCGTTGAATA AAGCAAATAA GAAACGCATC AAAGACATTC GTCGCAAAAT
     5521 CGTTGACATC AAGATCGCTA AGAAAAGCAA GTTTTATACC TGGGATGCTT TCAAGGCTAT
     5581 CTTTAAAAGC AACCGCATTC CGAAGATCGC AGAGTTGCGT AAAGAAATTA AAGCGATTCG
     5641 TAAAGAGAAC AAAACCGATT TGAAGCCGCT GCGTAAGGCC ATTAAGGCGG CGAAAAAGGC
     5701 TGGCCGCAAA GAAATCAGCA CCATTCTGAA GGACAACCAG TTCGCAAAGA GCCTGTTTGT
     5761 CGGTAGCCTG CGCTTTGGCA TCAACCGCCT GGTTTACATC AGCGGTTTTT ATCTGCTGTT
     5821 CGCGATCATT CTGGCTGTTA CGATCCCTTC CCTGGCGGGC GTCGGTATTA CCCTGCTGAT
     5881 CGGTGTTTTT GTGGCGAATA TTGTCATTTT AACGATGAGC CTGCCGCTGC TGATTTGGTT
     5941 GGAGAAACGC CGCATTGTGA GCAACTATGG TCGTAAAGAG TTCATTAGCC AAACGAATGT
     6001 CAGCCAGGAA GAACAAATCG TTAAAGACAT CAACGATTGA AGAGCTTAGA GACCCGCTGA
     6061 TGGTAATTAG CCCT
//