LOCUS       Exported     4361 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4361
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4327
                     /label="Mfl278"
                     /note="In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance."
                     /translation="MAGIILPRRTSKRASTNLNIVEIKDYITLENELKKYIKDRKALIKIKEAYLFAEKMHSEQKRKNGDPYIYHPLSTAYYLAQLQMGPKTIKAGLLHDVVEDTPVKIEEIEEKFGKEIASLVESVTKVSYFAEENREQIKSEYLRKLYISMAKDIRVIIIKIADRLHNMLTIENLPEEKQRVVAKETLTIYAAIAHRIGMKNAKSKLEDMAFKVLNPNDFTDIENLIEKGRESREDNIAKTIDDISFFLRKEKHIKIIDIFGREKTIYSIYRKMNVNGKQFEELHDLVAIRIIAKSTDDCYKILGYLHQKYLPLSGRFKDYIATPKNNVYQSLHTTLSNSKGMFFEVQIRTQQMDDVAETGAAAHWRYKEGEVVDIAKRQKQIDEQIDIFSRILDLTDQINEEENSKEKELENQLQKDVFGSMIYVLTPTQNVITLPYGATVLDFAYRIHTEIGEKTTGAKIDGIFSPINTVLESGQVVEIKTSSKQQPTHEWLKIATTSNAKNRIRKYLGNKLKEDNSFDEDRKELARKTENLINSYINQKEIKWKRKSQSEILEAVKKAGYANLEEFLINVGKGEISIVEATDKFFINHNFSKDEEALRSIQSKTINDRSLKNDIVIDGITNIKTSIASCCLPIPYEDVIGYVAKSGNGIKVHLKECYNLYTTEETKRLVQVQWNSAVAENSLYTTKLKYFATDRPNLLYDISRALSNLKATTINVKLGVDDKSLLVNGELTIKVKSSAQLNQIILTIKSIANIIDCERSVKSIK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGGGGCC GTCAATATCA GGGTCTCAAA TGGCGGGTAT
     2041 CATCCTGCCG CGTCGTACTA GCAAACGTGC AAGCACCAAT CTGAACATTG TGGAAATTAA
     2101 GGATTACATC ACGCTGGAAA ATGAGCTGAA AAAATATATC AAAGACCGCA AGGCGCTGAT
     2161 TAAAATTAAG GAAGCCTATC TGTTTGCTGA GAAAATGCAC AGCGAACAAA AGAGAAAAAA
     2221 TGGCGACCCG TATATTTATC ATCCGTTGTC GACGGCCTAC TACCTCGCGC AGCTGCAAAT
     2281 GGGTCCTAAG ACTATCAAGG CTGGTCTGCT GCACGACGTT GTTGAAGATA CCCCGGTTAA
     2341 AATTGAAGAA ATTGAGGAGA AATTTGGTAA GGAGATTGCG TCCCTGGTGG AGTCCGTGAC
     2401 GAAGGTGAGC TATTTCGCAG AGGAAAATCG CGAGCAGATC AAAAGCGAGT ATCTGCGCAA
     2461 GCTGTATATT AGCATGGCGA AAGACATTCG CGTCATCATT ATCAAGATTG CGGACCGCCT
     2521 GCATAACATG CTGACCATTG AAAATCTGCC TGAGGAGAAA CAACGCGTCG TTGCTAAAGA
     2581 AACGCTGACC ATTTATGCAG CAATCGCGCA TCGTATTGGC ATGAAAAACG CAAAGTCGAA
     2641 ACTGGAGGAT ATGGCATTTA AAGTTCTGAA TCCGAATGAT TTCACGGACA TCGAGAATCT
     2701 GATTGAGAAA GGCCGCGAAT CCCGTGAGGA CAATATCGCT AAAACTATCG ACGATATCAG
     2761 CTTCTTTCTG CGTAAGGAGA AGCACATCAA GATCATTGAC ATCTTCGGTC GTGAGAAAAC
     2821 CATCTACAGC ATTTACCGTA AAATGAACGT GAACGGTAAG CAATTTGAAG AACTGCACGA
     2881 CCTGGTTGCA ATCCGTATTA TTGCGAAAAG CACCGACGAC TGTTATAAAA TCCTGGGCTA
     2941 TCTGCATCAG AAGTATCTGC CGCTGAGCGG TCGCTTTAAA GACTACATTG CTACCCCGAA
     3001 AAACAACGTC TATCAATCCC TGCACACTAC GCTGAGCAAT AGCAAAGGTA TGTTCTTCGA
     3061 AGTCCAGATT CGTACGCAAC AAATGGACGA CGTTGCCGAA ACCGGCGCGG CGGCCCATTG
     3121 GCGTTATAAG GAAGGCGAAG TTGTGGATAT CGCCAAACGT CAGAAGCAGA TTGATGAACA
     3181 GATTGATATT TTCAGCCGCA TTTTGGATTT GACCGATCAG ATCAACGAGG AGGAGAATAG
     3241 CAAGGAAAAG GAACTGGAGA ATCAATTGCA GAAGGACGTT TTCGGCAGCA TGATCTATGT
     3301 GCTGACCCCG ACCCAGAACG TTATTACCCT GCCGTATGGC GCCACGGTTC TGGACTTCGC
     3361 ATACCGTATT CACACGGAAA TTGGTGAAAA GACCACCGGT GCGAAGATCG ACGGCATCTT
     3421 TTCTCCGATT AACACCGTAT TGGAGAGCGG TCAGGTGGTT GAGATCAAAA CGAGCAGCAA
     3481 ACAGCAGCCG ACCCACGAGT GGTTAAAAAT TGCGACCACC TCCAACGCGA AAAATCGTAT
     3541 TCGCAAATAT CTGGGTAACA AACTGAAAGA GGACAACAGC TTTGACGAGG ACCGTAAAGA
     3601 ACTGGCGCGC AAGACGGAGA ACCTGATTAA CTCGTACATT AATCAAAAGG AAATTAAATG
     3661 GAAACGTAAG TCTCAGAGCG AGATTCTGGA AGCGGTCAAG AAAGCGGGCT ATGCGAACTT
     3721 GGAAGAGTTC CTGATCAACG TTGGCAAGGG TGAGATTTCC ATTGTTGAGG CGACGGACAA
     3781 ATTCTTTATC AACCACAACT TCAGCAAAGA TGAGGAGGCA CTGCGCAGCA TCCAGAGCAA
     3841 GACTATTAAC GATCGTAGCC TGAAAAACGA CATTGTCATC GACGGCATTA CCAATATTAA
     3901 AACTTCGATC GCCAGCTGTT GTTTGCCGAT CCCGTACGAG GATGTTATCG GTTACGTGGC
     3961 GAAGTCCGGT AACGGTATTA AGGTTCACCT GAAAGAGTGT TATAATCTGT ATACCACTGA
     4021 AGAAACGAAA CGTCTGGTGC AAGTTCAGTG GAACTCCGCG GTTGCAGAAA ATTCCCTGTA
     4081 TACTACGAAG CTGAAATACT TCGCAACCGA CCGTCCGAAT CTGCTGTACG ACATTAGCCG
     4141 CGCTTTGAGC AACCTGAAGG CGACCACCAT CAATGTTAAA CTGGGTGTTG ATGATAAAAG
     4201 CCTGCTGGTG AATGGTGAAC TGACGATCAA GGTTAAGAGC AGCGCCCAGC TGAACCAGAT
     4261 TATCCTGACC ATCAAGTCTA TCGCAAATAT CATTGACTGT GAACGTAGCG TGAAAAGCAT
     4321 CAAGTGAAGA GCTTAGAGAC CGACGACTGC CTAAGGACAT T
//