LOCUS       Exported     3953 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3953
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3919
                     /label="infB"
                     /note="One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex."
                     /translation="MAKNIKTNKKPQQVNKKEMSKQHAKQIKQQLNETVATGIIDGVFVYTEALSIADFANQIGKSVAEILKYFFAQGLMLNQNVVLSEEQMAELALEFGFDFRKEESLTKENFFEALDASEEDKPEDLEHRAPIVTIMGHVDHGKTTLLDSIKNTNVVGGEAGGITQAIGAYQVKNKDGKKITFIDTPGHEAFSEMRSRGANVTDIVILIVAADDGVMPQTEEAIDHAKLANVPIIVFINKCDKPGADPERVKAELMKYEIVAEEYGGDIPFVQGSAKQKIGLDQLEETILLIAEMQDYKANPNKLAKGVVIEAHLDKAKGPVASILVKEGTLDIRDMIIAGTTYGNIKHMEDETNKKVLKAGPSKPVVVYGLNEVPSAGDKFIVMNDEKMARTIAEAQAEKKLAAERQSNQIFSLDSIKKHIDDGELKAINLIVKADTQGSVEALKGSLTKIDIPGVKLNIIRASVGTITLSDVTLASTVTDGIVLIYGFNVRPDAVVRKKAEEEGIEIRLHNIIYKVIEELEDAAKGMLDPEYKEVVTGSAEIRATFKHSDIGTIGGFHITDGSIERKSKVRIIRNGIVIYTGELATLKHLKDDIKEAKINSEGGLTIKNFNDIKEGDIVEGYKEEEVKK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAACGATGAT GCTCACTCTC GGGTCTCAAA TGGCTAAGAA
     2041 TATCAAGACC AACAAGAAGC CGCAACAGGT GAACAAGAAA GAAATGAGCA AGCAGCACGC
     2101 AAAGCAGATC AAACAGCAGC TGAATGAAAC GGTGGCAACC GGCATCATCG ACGGCGTTTT
     2161 CGTCTATACG GAGGCACTGT CTATCGCGGA CTTCGCTAAC CAAATCGGCA AGAGCGTAGC
     2221 TGAGATCCTG AAGTACTTTT TCGCACAAGG TCTGATGCTC AACCAGAACG TCGTGCTGTC
     2281 CGAGGAGCAA ATGGCCGAGC TGGCGCTGGA ATTCGGCTTC GATTTCCGTA AGGAGGAAAG
     2341 CCTGACCAAG GAGAACTTTT TCGAGGCACT GGACGCTAGC GAGGAAGATA AGCCGGAGGA
     2401 CCTCGAGCAT CGTGCGCCAA TTGTGACGAT TATGGGTCAC GTTGACCACG GCAAAACGAC
     2461 GCTGCTGGAC AGCATTAAGA ATACCAACGT CGTCGGCGGT GAAGCGGGTG GTATCACCCA
     2521 AGCCATTGGT GCTTACCAGG TTAAAAACAA GGACGGTAAG AAAATCACGT TCATCGACAC
     2581 GCCGGGCCAT GAGGCCTTCA GCGAGATGCG TAGCCGTGGT GCGAACGTTA CGGACATTGT
     2641 TATCCTGATT GTCGCAGCAG ACGATGGCGT CATGCCGCAG ACCGAGGAGG CCATCGATCA
     2701 TGCCAAGCTG GCGAATGTCC CGATCATCGT TTTTATCAAC AAGTGTGACA AGCCGGGTGC
     2761 CGATCCGGAG CGTGTCAAGG CAGAGCTGAT GAAATATGAA ATTGTAGCAG AAGAGTATGG
     2821 TGGTGATATC CCGTTTGTGC AAGGTAGCGC AAAACAGAAG ATCGGCCTGG ATCAACTTGA
     2881 AGAAACCATT CTGCTGATCG CGGAGATGCA AGACTACAAA GCGAACCCGA ATAAACTTGC
     2941 TAAAGGTGTG GTGATTGAAG CGCATCTGGA TAAAGCTAAG GGTCCGGTTG CATCCATCCT
     3001 GGTCAAAGAG GGCACGTTGG ATATCCGTGA TATGATTATT GCCGGTACCA CCTACGGTAA
     3061 CATCAAGCAC ATGGAAGATG AAACCAACAA GAAAGTGTTG AAGGCCGGTC CGAGCAAACC
     3121 GGTTGTGGTG TACGGTCTGA ATGAGGTTCC GAGCGCCGGT GACAAATTTA TCGTGATGAA
     3181 CGATGAGAAG ATGGCGCGTA CGATCGCGGA AGCCCAGGCT GAGAAAAAGC TGGCGGCCGA
     3241 GCGTCAGAGC AACCAAATCT TCTCGTTGGA CAGCATTAAG AAACATATTG ACGACGGTGA
     3301 ACTGAAAGCA ATTAACCTCA TCGTTAAGGC AGATACCCAG GGTTCGGTGG AAGCACTGAA
     3361 AGGCAGCCTG ACGAAAATTG ACATTCCGGG TGTTAAACTG AACATCATCC GTGCCAGCGT
     3421 GGGTACCATT ACTCTGTCCG ACGTGACCCT GGCCAGCACG GTGACTGACG GCATCGTCCT
     3481 GATTTATGGC TTTAATGTTC GTCCGGACGC CGTGGTGCGC AAGAAAGCGG AAGAAGAAGG
     3541 TATTGAGATC CGTTTGCACA ACATCATCTA CAAAGTAATT GAAGAACTGG AGGATGCGGC
     3601 GAAGGGTATG CTGGACCCGG AATATAAGGA GGTTGTTACT GGCAGCGCAG AGATTCGCGC
     3661 CACGTTCAAA CACAGCGACA TTGGTACCAT TGGTGGTTTC CATATTACCG ATGGCTCCAT
     3721 TGAACGCAAA AGCAAAGTTC GCATTATCCG TAACGGTATC GTCATCTACA CCGGTGAACT
     3781 GGCTACGCTG AAGCATCTGA AGGACGATAT TAAGGAGGCC AAAATCAATA GCGAGGGCGG
     3841 TCTGACGATT AAGAACTTCA ATGATATTAA AGAAGGTGAC ATCGTGGAAG GTTATAAAGA
     3901 AGAGGAGGTC AAAAAGTGAA GAGCTTAGAG ACCCGTGAAT CTTGACCTGT GGT
//