LOCUS       Exported     4571 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4571
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4537
                     /note=""
                     /translation="MKKRIFNKFQPYLNEFEKEKIALVEEKITGDNERIANDVSVDLIIILESKMMSILEKYDIYCPLDERGAKNLFDKIRSLYIREKKLESEKFTNVNIPKIIYSTFEYIRNWRNNDGGHASEFVINRSFMDTIHLLKCFDIVLSFLINFFDDLDFEINEFDEKGLLSSWNKRGHFIDEILEEDKKLDTTSIKLGKINLSSFVLNSEISFFIPSYQRKYRWESETCLELIENIISKIDQIDDEYFGTIAVTIEESKHNEKIRTIRLIDGQQRVTTSLIIFRAIYDVWNDKKNNSYEETVMDTPLELEKTFKEIGCAEKYKNVTGVKEENEALNFILNSNVSYVERLKTINSFELHNKSLAAKNYNAIHDRIKELNQDELLSFYNRYAYKFLISCVDFNKTPAEEMEIFETLNSKGTELDSFDMIKNFLFNLVDKEIYINNELEITRIFNDYISFNDMKLDEAKTRKVQENFLFGFCEYKMLNFKASDNTLSKNKKSILKHFKKIYEGKQNLSLEEYKKIVSEIGKYVFITKSFISKSYENDTNDILYPIRYNVSNISHKEVSIFILYYFIDLYAKNNWDSYNKTLNYSEKVNLMKDTLFEFERWLIALLQVYGTGQSLTKPILRLFRFLNTFDIDNHSVQSEIPNMIRKWLNLEATDAFAFLNNDQRRLLLENNELKMPNKDLFFENLINKKVQDKNVAMVILKRLESFLINNWEIKRDKNSLEHIMPRTIKKTKWIEYLKENESLTEKDILEKHSVYLDKLGNYMILDKSKENSKISNNDFEEKRKQYILWSNPLAELIFDYNEKKNLNNINKFGFDEIQERTVALAKIISENIYYK"
                     /label="Mfl305"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GACAACGAGC AGACCGAATA GGGTCTCAAA TGAAAAAACG
     2041 TATTTTTAAC AAATTTCAAC CGTACCTGAA CGAATTCGAG AAAGAGAAGA TTGCGTTGGT
     2101 GGAAGAAAAA ATCACGGGCG ACAACGAGCG TATTGCAAAC GACGTGTCCG TTGACTTAAT
     2161 TATTATTCTG GAGAGCAAGA TGATGTCAAT CCTGGAAAAG TATGATATTT ATTGTCCGCT
     2221 GGACGAGCGT GGCGCCAAAA ACCTGTTTGA TAAAATCCGT AGCCTGTACA TCCGCGAAAA
     2281 GAAGCTGGAA TCCGAAAAGT TTACCAATGT TAACATCCCG AAGATTATCT ATTCTACGTT
     2341 CGAATATATT CGTAATTGGC GTAATAATGA CGGTGGTCAT GCGTCTGAGT TTGTGATCAA
     2401 CCGTTCCTTT ATGGATACGA TTCATCTGCT GAAGTGTTTT GACATTGTGC TGAGCTTTCT
     2461 GATCAACTTT TTTGATGACT TGGATTTTGA AATTAACGAA TTCGACGAGA AAGGCCTGCT
     2521 GAGCAGCTGG AATAAACGCG GCCACTTCAT TGACGAGATT CTCGAAGAGG ACAAAAAGCT
     2581 GGACACTACC AGCATCAAGC TGGGTAAAAT TAATCTGTCC AGCTTTGTTC TCAACAGCGA
     2641 AATCAGCTTC TTTATTCCGT CGTACCAACG CAAATACCGT TGGGAGAGCG AAACTTGCCT
     2701 GGAACTGATT GAAAATATCA TCTCGAAGAT CGATCAGATT GACGACGAAT ATTTCGGCAC
     2761 TATCGCGGTT ACCATTGAAG AAAGCAAGCA CAATGAGAAG ATCCGTACCA TCCGTCTGAT
     2821 TGACGGCCAG CAGCGTGTCA CCACCAGCCT GATCATCTTT CGTGCGATCT ATGACGTCTG
     2881 GAATGATAAG AAGAACAATT CCTATGAAGA AACTGTGATG GACACCCCGC TGGAACTGGA
     2941 AAAGACGTTT AAGGAAATCG GTTGCGCGGA GAAGTACAAA AATGTTACCG GTGTCAAAGA
     3001 GGAAAATGAG GCTCTGAACT TTATCCTGAA TTCTAATGTC TCGTACGTTG AGCGTTTAAA
     3061 GACGATCAAC TCCTTTGAGC TTCACAACAA GAGCCTGGCG GCAAAAAACT ATAACGCAAT
     3121 TCATGACCGT ATTAAAGAAC TGAACCAAGA CGAACTGCTG TCCTTTTACA ACCGTTACGC
     3181 CTATAAGTTT CTGATCAGCT GTGTGGACTT CAACAAAACC CCGGCCGAAG AAATGGAAAT
     3241 CTTCGAAACG CTGAATTCCA AAGGCACCGA GCTCGACAGC TTTGATATGA TTAAAAATTT
     3301 TCTGTTCAAC CTGGTTGATA AGGAGATCTA TATTAACAAC GAACTGGAGA TCACGCGTAT
     3361 CTTCAACGAC TACATTAGCT TCAATGATAT GAAACTGGAT GAAGCGAAAA CCCGCAAAGT
     3421 CCAGGAGAAC TTCCTGTTCG GCTTCTGCGA GTATAAGATG CTCAATTTTA AAGCGAGCGA
     3481 TAATACGTTG TCCAAGAACA AGAAATCCAT CCTGAAGCAT TTCAAGAAGA TTTACGAAGG
     3541 CAAACAGAAC CTGAGCTTGG AGGAGTACAA GAAGATCGTG AGCGAGATTG GCAAATACGT
     3601 TTTCATTACG AAATCCTTCA TTAGCAAATC GTACGAGAAC GACACGAATG ATATCCTGTA
     3661 CCCGATCCGT TACAACGTCA GCAACATTAG CCACAAAGAG GTGTCCATCT TCATTCTGTA
     3721 TTACTTTATC GATCTGTACG CGAAAAATAA TTGGGATAGC TACAACAAGA CGCTGAATTA
     3781 TAGCGAGAAA GTGAATTTGA TGAAAGATAC GCTGTTTGAG TTTGAACGTT GGCTGATTGC
     3841 ACTGCTGCAA GTTTATGGCA CCGGTCAATC CCTGACCAAA CCGATTTTGA GACTCTTTCG
     3901 CTTCCTGAAT ACCTTCGACA TTGATAATCA CTCCGTACAG AGCGAAATTC CGAATATGAT
     3961 CCGTAAGTGG CTGAATCTGG AGGCGACGGA TGCTTTCGCG TTCCTGAACA ATGATCAACG
     4021 TCGTCTGCTG CTGGAGAACA ATGAACTGAA AATGCCGAAT AAAGATCTCT TCTTCGAAAA
     4081 CCTGATTAAT AAGAAAGTGC AGGATAAGAA CGTGGCTATG GTCATCCTGA AGCGTCTGGA
     4141 GAGCTTCCTG ATCAATAATT GGGAGATTAA ACGTGATAAG AACAGCCTGG AGCATATCAT
     4201 GCCGCGCACC ATTAAAAAGA CGAAATGGAT CGAGTACTTG AAAGAAAACG AGTCTTTGAC
     4261 TGAAAAAGAC ATCCTGGAGA AGCACTCCGT TTACTTGGAT AAACTGGGCA ATTACATGAT
     4321 TCTGGATAAG AGCAAAGAGA ACTCTAAGAT CAGCAATAAT GACTTTGAAG AAAAACGCAA
     4381 GCAGTACATT CTGTGGAGCA ACCCGCTGGC GGAGTTAATC TTTGACTATA ATGAGAAAAA
     4441 GAACTTAAAC AACATTAATA AGTTCGGTTT CGACGAAATC CAAGAGCGTA CGGTTGCACT
     4501 GGCCAAGATT ATCAGCGAGA ACATCTACTA CAAGTGAAGA GCTTAGAGAC CCTATCGGTG
     4561 CATCATCTCG T
//