LOCUS       Exported     3277 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3277
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1315..1362
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3210..3226
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3227..3258
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1243
                     /translation="MSDKIKVVELFAGVGGFSLALKKSKGNYEVIFSNQWEPSTKNQFAFNALNKNFKKHILSNEDIQFAKEKLPNDFDLLVGGFPCQDYSVATTNSKGIEGKKGVLWWEISWILEKHKPNFVFLENVDRLLKSPSNKRGRDFAIILKDLDEKGYNVEWMNINAGDYGYVQRRRRVFILAWRKEKLNIQKELNISDAFHKGIFRKGFKVKKFILEKEIDLNIFSDKVDVTENYNYGKFLNFGVMIDGKIISGDFVPLFKGKQKKLKDILEDKVDDSYFLTEEQIQKISILKDSKKIPRTKPNGEKYFYSEGKMSILENINMPSRTMLTSEGTINRASHFIEDTNSKGIKGIRRITPLEAERINGFTDNWTSDVMTERQRYFCMGNALVVPLVAKIADCLSEKNNEIKK"
                     /label="Mfl308"
                     /note=""
     primer_bind     complement(1367..1383)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1450..2038)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2229..3089)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3090..3194)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AATGCAAAGC TATTAGCGCG GGTCTCAAAT GAGCGATAAA ATCAAGGTTG TGGAACTCTT
       61 TGCCGGCGTA GGTGGTTTTA GCCTGGCGCT GAAGAAGTCA AAAGGTAACT ACGAAGTCAT
      121 TTTCAGCAAC CAATGGGAAC CGAGCACTAA GAATCAATTC GCCTTCAACG CTCTGAATAA
      181 AAACTTCAAA AAACACATTT TGTCGAATGA GGACATCCAA TTCGCGAAAG AAAAGCTGCC
      241 GAACGATTTC GATCTGCTGG TTGGTGGTTT CCCGTGTCAA GATTATAGCG TAGCCACCAC
      301 CAACTCGAAA GGTATTGAGG GTAAAAAAGG TGTATTGTGG TGGGAAATCA GCTGGATTCT
      361 CGAAAAACAT AAGCCGAATT TTGTGTTTCT GGAGAATGTT GATCGCCTGC TGAAGTCTCC
      421 GAGCAACAAG CGTGGTCGTG ACTTTGCAAT CATTCTGAAG GACTTAGACG AGAAAGGTTA
      481 TAATGTCGAA TGGATGAATA TTAACGCCGG CGACTACGGC TATGTGCAAC GTCGTCGCCG
      541 TGTGTTTATC CTGGCATGGC GTAAGGAAAA GCTGAATATC CAGAAAGAGC TGAATATCAG
      601 CGACGCATTT CACAAGGGTA TTTTTAGAAA AGGCTTCAAA GTGAAGAAGT TCATCCTTGA
      661 GAAAGAAATT GACTTGAACA TCTTCAGCGA TAAAGTTGAC GTTACCGAGA ACTATAACTA
      721 CGGTAAGTTT CTGAACTTCG GCGTGATGAT CGACGGCAAA ATCATCAGCG GTGATTTTGT
      781 GCCGCTGTTC AAAGGTAAAC AGAAAAAGCT GAAAGACATT CTGGAAGATA AAGTTGATGA
      841 CTCCTATTTC CTGACGGAGG AGCAGATTCA GAAGATCTCT ATCCTGAAGG ACTCCAAGAA
      901 AATCCCGCGT ACGAAGCCGA ATGGTGAGAA GTACTTCTAT AGCGAGGGTA AGATGTCTAT
      961 TCTGGAAAAT ATTAACATGC CGAGCCGTAC GATGTTGACC AGCGAAGGTA CCATCAACCG
     1021 TGCAAGCCAC TTCATTGAGG ATACCAACTC GAAGGGTATT AAAGGTATTC GTCGTATCAC
     1081 CCCGCTCGAG GCTGAACGTA TTAATGGTTT CACGGATAAT TGGACGTCTG ATGTTATGAC
     1141 TGAGCGTCAG CGCTATTTCT GCATGGGCAA CGCCCTGGTG GTGCCACTGG TCGCGAAGAT
     1201 TGCGGATTGT TTGTCGGAGA AAAATAACGA GATCAAAAAG TGAAGAGCTT AGAGACCGGA
     1261 CCTTGACGCC TAATACTCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1321 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1381 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1441 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1501 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1561 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1621 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1681 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1741 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1801 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1861 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1921 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1981 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     2041 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     2101 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     2161 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     2221 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     2281 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2341 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     2401 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2461 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2521 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2581 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2641 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2701 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     2761 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     2821 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     2881 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     2941 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     3001 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     3061 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     3121 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     3181 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     3241 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG
//