LOCUS       Exported     4007 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4007
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3973
                     /label="Mfl309"
                     /note=""
                     /translation="MAKEVKYDESAIQVLEGLEAVRKRPGMYIGSTDVRGLHHLVWEIVDNSIDEALAGYCTEINVTLEKNGSVTVADNGRGVPIGMHSTGRPTPEVIFSVLHAGGKFGGSGYKTSGGLHGVGSSVVNALSKKFNVTIYRDKKVNEIEFTNGGKLNIPLTEVGTTNKTGTVVNFLPDDSIFNTTKFNFTTISERLKESALLNSGLKITISDKVNDKFVEYQFENGLVEFVKELSSEFTHITDPVVITGESKRIASEICIQYTEDFNETILGFANNVKTGDGGTHITGFKTGLVRAINDYAKNNKILKDKDPRLDSNDLREGLVAIVTVKIPEDLIEYEGQTKGKLGTPDAKTAVEQVTYDFMNFWLIENKVPATKIIEKAMLARRAREEARKARQAIRDSKGKKTTRAMLGKLTPAQGRKKEINELYLVEGDSAGGSAKSGRDRTFQAILPLRGKVINSEKAKLTELMKNEEIQTIITAIGAGIGQDFDVSDINYGKVIIMTDADTDGAHIQTLLLTFFYRYMKDLIINKHVFIALPPLYKLTFSDRKFIYLWDEQELADFAKTATKKYEIQRYKGLGEMNADQLWETTMNPAQRKLIVVTIEDALMAEKSFRTLMGEDAEKRKVWIQENVKFTLEDNDDAIILNEKQENN"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGAATTAC TGACCCCTCG GGGTCTCAAA TGGCAAAAGA
     2041 GGTCAAATAC GACGAGTCGG CGATTCAGGT GCTGGAAGGC CTGGAGGCTG TCCGCAAACG
     2101 CCCTGGTATG TACATTGGTA GCACTGATGT CCGTGGTCTG CATCATCTTG TGTGGGAAAT
     2161 TGTTGACAAC AGCATTGATG AGGCATTGGC GGGTTACTGT ACCGAGATTA ACGTTACTCT
     2221 CGAGAAGAAC GGCAGCGTGA CCGTTGCGGA TAACGGCAGA GGCGTTCCAA TCGGTATGCA
     2281 CAGCACCGGC AGACCGACCC CGGAGGTTAT TTTCTCCGTG CTGCATGCTG GCGGCAAATT
     2341 CGGTGGTAGC GGTTACAAAA CCAGCGGTGG TCTGCATGGC GTGGGCTCCA GCGTCGTTAA
     2401 CGCCCTGAGC AAAAAATTCA ATGTGACGAT TTATCGTGAT AAAAAGGTGA ATGAAATCGA
     2461 ATTCACCAAT GGTGGCAAAC TGAATATCCC ACTCACCGAA GTCGGCACTA CGAACAAGAC
     2521 CGGCACGGTC GTAAATTTTC TCCCGGACGA TAGCATTTTC AACACCACGA AGTTCAATTT
     2581 CACCACCATT TCTGAACGCC TCAAGGAGTC TGCGCTGTTA AACTCCGGCT TGAAAATCAC
     2641 CATCAGCGAT AAAGTCAATG ATAAATTCGT GGAGTACCAA TTTGAAAACG GTCTGGTGGA
     2701 GTTCGTGAAA GAACTGTCCA GCGAGTTCAC CCACATCACC GACCCGGTGG TTATCACGGG
     2761 TGAGAGCAAG CGTATCGCGA GCGAGATTTG TATTCAATAT ACCGAGGACT TCAACGAAAC
     2821 GATTCTGGGC TTTGCAAATA ACGTGAAAAC CGGCGACGGC GGTACGCATA TTACCGGTTT
     2881 TAAGACGGGT CTGGTCCGCG CAATTAACGA TTATGCCAAA AACAACAAGA TCTTGAAGGA
     2941 CAAAGACCCG CGCCTGGACA GCAATGATCT GCGTGAGGGT CTGGTGGCAA TTGTCACCGT
     3001 TAAAATCCCA GAGGACTTGA TTGAATACGA AGGCCAGACT AAAGGTAAAC TGGGTACCCC
     3061 GGACGCGAAA ACCGCTGTTG AGCAAGTGAC CTATGATTTT ATGAATTTCT GGCTGATTGA
     3121 AAATAAAGTT CCTGCCACCA AGATTATTGA AAAGGCTATG TTGGCGCGTC GTGCGCGTGA
     3181 GGAGGCGCGT AAGGCTCGCC AAGCGATCCG TGACTCGAAA GGCAAAAAGA CTACCCGCGC
     3241 CATGTTGGGT AAACTGACCC CGGCGCAAGG TCGTAAGAAG GAAATTAATG AGCTGTATCT
     3301 GGTTGAGGGC GATTCGGCCG GTGGTAGCGC GAAAAGCGGT CGCGACCGCA CCTTTCAAGC
     3361 GATCCTGCCT CTGCGCGGTA AAGTGATCAA CTCTGAGAAA GCGAAGCTGA CGGAACTGAT
     3421 GAAGAATGAG GAGATCCAAA CGATTATTAC CGCGATTGGC GCCGGTATTG GCCAGGATTT
     3481 CGACGTTAGC GACATCAATT ACGGTAAAGT GATTATTATG ACCGACGCAG ACACCGACGG
     3541 TGCCCATATC CAAACCCTGC TGCTGACGTT CTTTTACCGT TATATGAAAG ACCTGATCAT
     3601 TAACAAGCAT GTTTTCATTG CGCTGCCGCC GCTGTATAAG CTGACCTTTT CGGACCGTAA
     3661 GTTCATTTAT CTGTGGGATG AACAAGAACT GGCGGATTTC GCCAAGACGG CAACCAAAAA
     3721 ATATGAGATT CAGCGTTACA AGGGTCTGGG TGAGATGAAT GCGGACCAGT TGTGGGAAAC
     3781 GACTATGAAC CCTGCCCAAC GTAAGCTGAT CGTTGTTACG ATTGAAGATG CACTGATGGC
     3841 AGAGAAAAGC TTCCGCACCC TGATGGGTGA GGACGCCGAG AAGCGTAAGG TGTGGATCCA
     3901 AGAGAATGTC AAGTTTACGC TGGAGGATAA CGATGATGCA ATCATCCTGA ACGAAAAACA
     3961 AGAAAACAAC TGAAGAGCTT AGAGACCGTG TAGTGTTCCC CATACGT
//