LOCUS       Exported     4790 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4790
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4756
                     /translation="MAKKIDNSNIESEKGIISYALEDLMGERFGRYAKYIIQERALPDARDGLKPVQRRILYAMNELNLTYDKPYKKSARVVGEVIGKYHPHGDTSIYDAMVRMSQWWKLGMPLIDMQGNNGSIDGDNAAAMRYTETRLAKISDLMLDDLNKNTVKFAPNFDDSEKEPTVLPSYFPNILVNGSTGIAAGYATNMPPHNLGEIIDATIKLIRTPNTRIDTILEIVKGPDFPTGGTVQGRNGIKDAFTTGKGKVIVNSKWHEEDNNIIIDEIPYEVVKQDLVKKIGDVIDANPGLGILEVRDETDRNGLRIAIDLSDKANLDTVRKFLFKSTPLSISYNYNNVAIVDLQPKQLGIIELIHAYIAHYKEVFTFRTQFDLNKAEKRLEIILGLIKAMSILDQVISVIRSSTNRSDAIENLISKFVFSQPQAAAIVDMRLYRLTSTDVVKLQNEKEELDVNIAKLKAILNSEEVMDNEIINRLREVKKQFPTPRRSIVEDSIENLDVEQKEVLVEHEYNLWISKDGYLKAIESKLISKNDPAVFGRKPNDMWIAAGEVSNLQHLILVTNKGTYYSIPLYKLPMSKWRDMGVHVNTIATMDPSEHIVSAFVVKDFAEALQQILITSKNGNIKRIPVKDLETKIFTRAFRIMKLDAVDEIVSASLITSKTRTCGIITRNGYGVRYHIEDIPVQGTNSKGVKAANLKEDYIVAGLGLTSEDTIMYLTEKDGVKKFRNEDLPIYIRPKRGIRVLPERKRGTEYITFAFNFNANEENIIKAIDTQDMYQEVNVNKYRHIELTSVTGDFDIKDVAFASLSEINKVKPNDMPPGALNADDDAEGYVSKEEIRARQEANKGKVSAKVVVSKNIKEESEKRIQSLTGGLSDLLGDISSVLGTPSKSKPKKEVKDEFQLDLSDLLEE"
                     /label="Mfl310"
                     /note="Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule."
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACACGTG GCAAACATTC CGGTCTCAAA TGGCGAAAAA
     2041 GATCGACAAT TCCAATATCG AAAGCGAGAA GGGTATCATC TCTTACGCAC TGGAAGATTT
     2101 GATGGGCGAA CGCTTCGGTC GTTATGCCAA ATATATCATC CAAGAGCGCG CTCTGCCAGA
     2161 TGCACGTGAC GGTCTGAAAC CGGTCCAACG TCGTATCCTG TATGCTATGA ATGAACTGAA
     2221 CCTGACCTAT GACAAGCCGT ATAAGAAAAG CGCACGTGTT GTTGGTGAAG TTATCGGCAA
     2281 ATACCATCCG CATGGTGATA CGAGCATCTA TGATGCCATG GTTCGCATGT CCCAGTGGTG
     2341 GAAGCTGGGT ATGCCGCTGA TTGATATGCA AGGTAACAAC GGCAGCATTG ACGGTGACAA
     2401 CGCGGCAGCA ATGCGTTATA CCGAAACCCG TCTGGCGAAG ATTAGCGACC TGATGCTGGA
     2461 CGACCTGAAT AAAAATACCG TGAAGTTCGC TCCAAATTTT GATGATTCCG AGAAAGAGCC
     2521 GACCGTCCTG CCGAGCTACT TTCCGAATAT TCTGGTCAAT GGTAGCACGG GTATTGCCGC
     2581 GGGCTATGCG ACCAATATGC CGCCGCACAA CTTGGGTGAG ATTATCGATG CGACGATTAA
     2641 ACTGATCCGC ACGCCGAATA CCCGCATCGA CACCATTCTG GAGATTGTTA AAGGCCCGGA
     2701 CTTCCCGACG GGTGGTACGG TCCAGGGCCG CAACGGTATC AAAGACGCAT TCACTACCGG
     2761 CAAAGGTAAA GTGATCGTGA ATAGCAAATG GCATGAAGAG GATAACAACA TTATCATCGA
     2821 CGAAATCCCG TACGAGGTTG TCAAGCAGGA CCTGGTGAAG AAAATCGGTG ACGTTATCGA
     2881 CGCAAACCCG GGCCTGGGCA TTTTAGAGGT GCGTGATGAA ACGGACCGTA ATGGCCTGCG
     2941 TATTGCGATT GATCTGTCGG ATAAGGCCAA CCTGGACACG GTACGTAAGT TCCTGTTCAA
     3001 GAGCACGCCG CTGAGCATCA GCTATAATTA CAACAATGTC GCGATCGTGG ATCTGCAGCC
     3061 GAAACAACTG GGCATCATTG AGTTGATTCA CGCGTACATT GCGCATTATA AGGAGGTATT
     3121 TACCTTTCGT ACGCAGTTCG ACCTGAACAA GGCGGAAAAA CGCCTGGAAA TCATTCTGGG
     3181 TTTGATCAAG GCAATGAGCA TCCTGGATCA AGTGATCAGC GTTATCCGTA GCAGCACTAA
     3241 CCGCTCGGAT GCGATCGAGA ACCTGATTTC CAAATTCGTT TTCAGCCAAC CGCAGGCGGC
     3301 AGCGATTGTT GACATGCGTC TGTATCGTCT GACGAGCACC GACGTCGTTA AGCTGCAGAA
     3361 CGAAAAGGAG GAGCTGGATG TGAATATCGC CAAACTGAAG GCAATTCTGA ACAGCGAGGA
     3421 AGTGATGGAC AACGAGATTA TTAACCGTCT GCGTGAGGTT AAGAAGCAGT TTCCGACCCC
     3481 GCGTCGTAGC ATCGTGGAGG ATAGCATCGA GAACTTGGAT GTCGAACAGA AGGAAGTCCT
     3541 CGTGGAGCAT GAATACAACC TGTGGATTAG CAAAGATGGC TATTTGAAGG CGATCGAAAG
     3601 CAAGCTGATC AGCAAAAATG ATCCGGCGGT TTTCGGTCGC AAGCCGAATG ATATGTGGAT
     3661 CGCAGCGGGC GAAGTTTCCA ATCTGCAGCA CTTGATCTTG GTGACGAATA AAGGTACGTA
     3721 CTACTCCATC CCGCTGTATA AGCTGCCGAT GAGCAAATGG CGTGACATGG GTGTTCATGT
     3781 TAACACCATT GCAACCATGG ACCCTAGCGA ACATATCGTG TCCGCTTTTG TCGTCAAAGA
     3841 CTTTGCAGAA GCACTGCAAC AGATCCTGAT TACCTCGAAG AATGGCAATA TTAAGCGTAT
     3901 CCCGGTGAAA GACTTGGAAA CCAAGATTTT CACGCGTGCC TTTCGTATCA TGAAACTGGA
     3961 CGCGGTGGAT GAGATCGTGT CCGCCAGCCT GATTACCTCC AAAACGCGCA CCTGTGGCAT
     4021 TATTACTCGT AACGGCTATG GTGTCCGCTA CCACATTGAG GACATTCCGG TTCAGGGTAC
     4081 CAACAGCAAA GGTGTTAAAG CAGCAAATTT GAAAGAGGAT TACATTGTTG CAGGCCTGGG
     4141 CCTGACGAGC GAGGATACCA TCATGTACCT GACGGAAAAG GATGGCGTGA AGAAATTCCG
     4201 CAACGAAGAT CTGCCGATTT ATATTCGTCC GAAACGCGGC ATTCGTGTAC TGCCGGAGCG
     4261 CAAACGCGGT ACCGAGTATA TTACCTTCGC GTTCAATTTC AACGCAAACG AGGAGAATAT
     4321 TATCAAGGCG ATCGATACCC AGGATATGTA TCAGGAAGTT AACGTGAACA AGTACCGTCA
     4381 TATTGAGCTG ACGTCTGTGA CGGGTGATTT TGACATCAAG GACGTCGCAT TCGCGTCCCT
     4441 GAGCGAGATC AATAAAGTTA AACCGAACGA TATGCCGCCG GGTGCGCTGA ACGCGGATGA
     4501 CGATGCGGAG GGTTATGTTA GCAAGGAGGA GATCCGTGCG CGTCAAGAAG CGAACAAAGG
     4561 CAAAGTCTCC GCTAAGGTGG TGGTGAGCAA AAATATCAAA GAAGAGAGCG AGAAACGTAT
     4621 CCAGAGCCTG ACGGGCGGCC TGTCTGATCT GCTGGGTGAT ATTAGCAGCG TCCTGGGCAC
     4681 CCCGTCTAAG AGCAAGCCGA AAAAAGAGGT TAAAGACGAA TTTCAACTGG ATCTGTCCGA
     4741 TCTGCTGGAA GAGTGAAGAG CTTAGAGACC GCTCGATGCC ATAGTTCCTT
//