LOCUS       Exported     4612 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4612
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2650..2697
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4545..4561
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4562..4593
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..2578
                     /label="Mfl312"
                     /note=""
                     /translation="MKNKKIIIYAPVNGVIGKIKDLKDGVFSEGMLGEGVYIIPESKEFYSPIDKGELKLVTATKHAYYFEHENGPNILMHISLDTEKHQGKPFKTLSNVGDKLTLATKIVSVDLQMCKKQDVNVATPILIDTKEFSEWKFKPLDLKGKVKRGQPIGEFVKVEEKKINEESNDLTTFEFKNKYEKSAESIYEYIGTESNYSKVYNCMTRLRFYVKDKQIVNTEAIKKMPIVKGVNWNGEEIQIIIGGEVQKVKDAFENYVSNKNISKTSKKQEGFEQKIKPKKPMKEAILGAISGIMVPTLPVFITVGMLMGLKAILVQAGAMPEIVTGIAKEGQVSLTDADVFSAIFFIITEIAFKFLGIFIGYNTMKYLGGNTIMSILIGLALANPYIWQGTSWPLFTIGSTTIKVQAFTSSIMPHIAANIIFFYLDKWIKTWMPSVVDIIFRPMIAFLTTMLLAFFIVGPSLFLVEQGLAVIVGWLGSIPFGIGVAIYAILWQPFVISGMHIAIAMPLSIGLMQGTPQVISPAIMYGCYGQLGAMIGVMIMTKNSQLKATCIANLTPGVIAITEPIIYGITLPKGRPFIAGCLGAGLAGLFSGILNVRLMHSGGYGIFSIVGYIAGGPMNVAMFVVSLLIAASSAAIICLFIYQERPSELKSIKKISRVIIKNYALKTGLKNNEVEKLLDEKLSLIQNFVKKDDIEKIKKVEDSYIKISKLEATIDLLNSKDEKAQQKLNEKMIRANEKNNLLNAKKIYDELVNFKQNSKIEKLINELNEVKKANVNLENWLKDHQDKFMELTNEILLKISKEIELNDINDLNSNYFNAIHSIDINYLITEKQETFFNQKNVKKNLLNNR"
     primer_bind     complement(2702..2718)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2785..3373)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3564..4424)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4425..4529)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GAAGAACAAG AAAATTATTA TCTATGCGCC
       61 GGTTAATGGT GTCATTGGTA AGATTAAAGA TCTGAAGGAT GGTGTCTTTT CCGAGGGCAT
      121 GCTGGGTGAA GGTGTCTATA TCATCCCGGA ATCGAAGGAA TTTTATAGCC CGATTGACAA
      181 AGGTGAACTC AAACTGGTGA CGGCGACCAA GCACGCCTAT TACTTTGAAC ACGAAAATGG
      241 CCCGAACATC CTGATGCACA TCAGCCTGGA CACGGAAAAA CATCAAGGCA AGCCTTTTAA
      301 AACCTTGTCA AACGTCGGTG ATAAACTGAC GCTGGCAACC AAGATTGTCA GCGTTGATTT
      361 ACAAATGTGC AAGAAGCAAG ATGTCAACGT TGCGACGCCG ATTCTGATCG ACACCAAGGA
      421 ATTCTCCGAG TGGAAGTTCA AGCCGCTGGA CCTGAAAGGT AAGGTCAAGC GTGGCCAGCC
      481 GATCGGTGAG TTTGTGAAGG TGGAAGAGAA GAAAATTAAT GAGGAATCTA ATGATCTGAC
      541 CACCTTCGAG TTTAAAAACA AGTACGAGAA AAGCGCGGAG AGCATTTATG AGTATATTGG
      601 TACCGAGAGC AACTATAGCA AAGTTTATAA CTGCATGACT CGCTTGCGTT TCTACGTGAA
      661 GGACAAGCAA ATCGTAAACA CCGAAGCAAT CAAGAAAATG CCGATTGTGA AGGGCGTCAA
      721 CTGGAATGGT GAGGAAATTC AGATCATCAT TGGCGGTGAG GTGCAGAAGG TTAAGGATGC
      781 GTTTGAGAAC TACGTCAGCA ACAAGAATAT TTCCAAGACG TCCAAAAAGC AGGAGGGCTT
      841 TGAACAGAAG ATCAAACCAA AGAAGCCGAT GAAAGAAGCA ATTCTGGGCG CAATTTCTGG
      901 TATCATGGTT CCGACGCTGC CTGTGTTTAT TACCGTCGGC ATGCTGATGG GTCTGAAAGC
      961 GATCCTGGTC CAGGCCGGTG CCATGCCGGA GATTGTTACC GGCATTGCGA AGGAGGGTCA
     1021 GGTTAGCCTG ACCGACGCGG ATGTTTTCAG CGCGATCTTT TTCATTATCA CTGAGATTGC
     1081 GTTCAAATTT CTGGGCATTT TCATCGGCTA CAATACGATG AAATATCTGG GTGGTAATAC
     1141 CATTATGTCC ATCCTGATTG GCTTGGCGCT GGCAAATCCG TACATTTGGC AGGGCACGAG
     1201 CTGGCCACTG TTTACCATTG GCTCCACGAC CATCAAGGTC CAGGCATTCA CGAGCAGCAT
     1261 CATGCCTCAC ATTGCGGCAA ACATCATTTT CTTCTATCTG GACAAGTGGA TCAAAACCTG
     1321 GATGCCGAGC GTCGTGGACA TCATCTTCCG CCCTATGATC GCGTTCCTTA CCACCATGTT
     1381 GCTGGCCTTC TTCATTGTTG GTCCGAGCCT GTTTCTGGTG GAGCAGGGCC TGGCCGTCAT
     1441 CGTGGGTTGG CTGGGCTCGA TTCCGTTTGG TATTGGCGTT GCTATTTATG CGATCCTGTG
     1501 GCAACCGTTC GTTATCTCCG GTATGCATAT CGCGATTGCA ATGCCGCTGA GCATCGGCCT
     1561 GATGCAGGGC ACCCCACAGG TGATCTCTCC GGCCATCATG TATGGTTGCT ACGGCCAACT
     1621 GGGCGCTATG ATTGGTGTGA TGATTATGAC CAAGAACAGC CAACTGAAAG CGACGTGTAT
     1681 TGCCAATCTG ACGCCGGGCG TGATCGCCAT CACTGAACCG ATCATTTATG GCATTACCCT
     1741 GCCGAAAGGC CGTCCGTTCA TTGCCGGCTG CCTGGGCGCA GGCTTGGCAG GCCTGTTCAG
     1801 CGGTATTCTG AACGTGCGTC TGATGCACTC GGGTGGCTAC GGTATTTTCA GCATTGTCGG
     1861 TTATATCGCG GGTGGTCCGA TGAACGTAGC AATGTTCGTC GTTAGCCTGC TGATTGCAGC
     1921 TAGCTCCGCG GCAATCATCT GTCTGTTCAT CTACCAAGAG CGTCCAAGCG AACTGAAGTC
     1981 CATCAAAAAG ATCTCTCGTG TTATTATCAA GAATTATGCA CTGAAAACCG GTTTGAAGAA
     2041 CAACGAAGTT GAGAAGTTGT TGGACGAGAA ACTCTCTCTG ATCCAGAACT TCGTTAAGAA
     2101 GGATGACATT GAAAAGATCA AAAAGGTTGA GGATAGCTAT ATTAAAATTA GCAAACTGGA
     2161 GGCTACTATC GATCTGCTGA ATAGCAAAGA TGAGAAGGCT CAGCAGAAGC TCAACGAAAA
     2221 GATGATTCGC GCAAACGAGA AAAATAATCT GCTGAACGCC AAGAAGATCT ACGATGAGCT
     2281 GGTTAACTTC AAACAAAACA GCAAGATTGA GAAACTGATC AATGAGCTGA ACGAAGTCAA
     2341 AAAAGCCAAC GTTAATCTGG AGAATTGGCT GAAGGATCAC CAAGACAAAT TCATGGAGCT
     2401 GACGAATGAA ATCCTGCTGA AAATCAGCAA GGAGATCGAG CTGAATGACA TCAATGACCT
     2461 GAATAGCAAC TACTTCAACG CGATTCACAG CATCGACATC AACTACTTGA TCACCGAAAA
     2521 GCAAGAAACC TTCTTCAACC AGAAGAACGT CAAGAAGAAC CTGTTGAATA ACCGCTGAAG
     2581 AGCTTAGAGA CCCCATCTTG CGTCGTGAGA ATCGCTGAGG TGTCAATCGT CGGAGCCGCT
     2641 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     2701 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     2761 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     2821 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     2881 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     2941 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     3001 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     3061 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     3121 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     3181 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     3241 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     3301 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     3361 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     3421 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     3481 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     3541 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     3601 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     3661 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     3721 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     3781 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     3841 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     3901 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     3961 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     4021 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     4081 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     4141 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     4201 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     4261 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     4321 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     4381 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     4441 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     4501 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     4561 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG
//