LOCUS Exported 2761 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2761 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 799..846 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2694..2710 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2711..2742 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..727 /label="Mfl316" /note="" /translation="MILEMRFFQMDHGYIYSYIENKILSNEFSADQKMLSENQICIKFNVSRTLARDVYKELVVDGYLYSIHGKGYFVNDKKYWMKNIGLDKKYPHSEYKNEILDFKIELPNWFVENYKLNKNDYNCFTKARYLNNKQRHLSYVFLNNKLILNMDLKEIKKSVVNYLKKDFEFKKSYNFIKIEKATKFDADFFSINENDFIVCQYSVIYHNDEIVLQCSLDKRPIEYLNFGYIANV" primer_bind complement(851..867) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(934..1522) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1713..2573) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2574..2678) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAAGGGGCCG TCAATATCAG GGTCTCAAAT GATTCTGGAA ATGCGCTTTT TTCAGATGGA 61 TCACGGTTAC ATTTACTCCT ATATTGAGAA CAAAATCCTG TCGAACGAGT TCTCTGCCGA 121 CCAGAAGATG CTGTCCGAAA ACCAAATTTG TATCAAGTTT AACGTTAGCC GCACCCTGGC 181 GCGTGACGTT TATAAGGAAC TGGTTGTTGA TGGTTACCTG TACAGCATCC ACGGTAAGGG 241 TTACTTCGTC AACGACAAGA AGTACTGGAT GAAAAATATC GGCCTGGACA AGAAGTACCC 301 GCACAGCGAG TACAAGAATG AAATCCTGGA CTTTAAGATT GAGCTGCCGA ACTGGTTCGT 361 CGAGAATTAT AAGTTGAACA AGAATGACTA CAATTGCTTT ACCAAGGCAC GTTATCTGAA 421 CAATAAACAG CGCCATCTGA GCTACGTTTT TCTGAATAAT AAGCTGATTC TGAACATGGA 481 TTTGAAGGAA ATCAAGAAAA GCGTGGTTAA CTACCTGAAA AAAGACTTCG AGTTCAAGAA 541 GTCCTACAAT TTTATCAAGA TTGAGAAAGC TACCAAATTT GACGCCGACT TTTTTAGCAT 601 TAATGAGAAC GACTTCATTG TGTGCCAGTA CAGCGTTATC TATCATAACG ATGAAATTGT 661 CCTGCAGTGT AGCTTGGATA AACGTCCGAT TGAGTACCTG AACTTTGGTT ATATCGCGAA 721 CGTGTGAAGA GCTTAGAGAC CCAGCCTCTG TGTTGTCTGA TCGCTGAGGT GTCAATCGTC 781 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT 841 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA 901 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA 961 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 1021 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 1081 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 1141 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 1201 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 1261 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 1321 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 1381 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 1441 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 1501 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT 1561 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 1621 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 1681 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT 1741 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA 1801 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA 1861 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA 1921 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA 1981 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG 2041 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 2101 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 2161 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 2221 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 2281 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 2341 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 2401 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 2461 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 2521 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 2581 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 2641 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC 2701 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA 2761 G //