LOCUS       Exported     4322 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4322
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4288
                     /label="Mfl317"
                     /note=""
                     /translation="MSIRDNITKKQYAYNCVEHERIFSIESNYAIALRLSDVWEEGNKLYVEVTFNNFQKAYLSFAEYENGIVNIRYWQKQIPVNRYYDKNHENMVTTKIKYKNDKNEIVISLKNGENIIITKNKFQLALVDVNGEIKTKTSLRPGWDHLEGYCTPPLGFKIDDNIQKPFMTMQLASEELIYGLGEKFNNFVKNGVQSTIFNVDNEAVSNGDYAYSGVPLVYSSRNWGLLLNTAHKTEWEIGSPTTESLSFMSTEECLDIILFSGDSLKDLVKQYTLFTGRITGVPDEAYGLWLNRLYYHNKKELFTEIENSIKNEYPIDVFTLDPKWIKNRYTKSCNFEFNDDQFGDLETMLSEVHEKGFKMCFWINPYLQIDNSEMWNEVYEKGYHVKKADGSIAHIWSGFGNYQETASAIDLTNPDAVVYWKNKIKDLMYKGVDFIKTDYGDSVDEDAVMFNGEKGTNFKNAYAELYLRYVYEATQEVKGIDKGFCLSRPGYIGTQKYVGKWAGDSASSFNELKMQLLSGLSNSLCGTVMWGTDIGGFLDINANEEDLYARWSQFGLLTPFSRYHGVGAREPWYFGEKDLNISREAAKLKRQLLPYYKIYEKEAIESGLPIIRPLVLEFPNDTIAAKIDDQFMLGENIMVAPILSNKKYERQVYFPEGNWIDFSDKKIYEGNKKYNIDCPIEKILIFVKENSIIPMIKNPMYKFNNIDNEKIKLRIYGNVLNETLKFRINDKDYSVEVRDNKIVNENEKFEVE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCAAA TGTCCATTCG
     2041 TGACAACATT ACCAAGAAGC AATACGCGTA TAATTGCGTC GAGCATGAAC GCATCTTTTC
     2101 CATCGAGTCT AACTATGCCA TTGCTTTGCG CCTGAGCGAC GTTTGGGAGG AAGGTAATAA
     2161 ACTGTACGTC GAAGTGACCT TTAATAATTT TCAGAAGGCC TACCTGTCAT TCGCGGAGTA
     2221 TGAGAATGGC ATTGTTAACA TCCGTTATTG GCAAAAACAA ATCCCGGTGA ACCGTTATTA
     2281 CGATAAGAAT CACGAGAACA TGGTGACTAC CAAGATCAAG TATAAAAACG ATAAGAACGA
     2341 GATTGTGATT AGCCTGAAAA ATGGCGAGAA TATTATTATC ACCAAGAACA AGTTCCAGCT
     2401 GGCTTTAGTC GATGTAAATG GTGAGATTAA AACGAAAACC TCCCTTAGAC CGGGTTGGGA
     2461 TCACCTGGAG GGTTACTGTA CGCCGCCGCT CGGTTTCAAA ATTGATGACA ATATCCAAAA
     2521 GCCGTTCATG ACCATGCAAT TGGCGAGCGA AGAACTGATC TACGGTCTGG GTGAAAAATT
     2581 TAATAATTTC GTGAAAAACG GTGTTCAAAG CACGATCTTC AATGTGGATA ACGAGGCGGT
     2641 CAGCAATGGT GACTACGCAT ATAGCGGTGT TCCGCTGGTG TACAGCTCTC GCAATTGGGG
     2701 TCTGCTGCTG AACACTGCGC ATAAGACCGA GTGGGAGATC GGCAGCCCAA CGACCGAGAG
     2761 CCTGAGCTTC ATGTCTACCG AGGAATGTTT GGACATTATT TTGTTCTCTG GCGACAGCCT
     2821 GAAAGACCTG GTTAAGCAAT ACACCCTGTT TACCGGCCGT ATCACTGGTG TTCCGGACGA
     2881 GGCGTACGGT CTGTGGCTGA ACCGCCTGTA CTACCACAAT AAAAAAGAAC TGTTCACCGA
     2941 GATTGAGAAC AGCATCAAAA ATGAGTACCC GATTGATGTG TTCACCCTGG ACCCGAAATG
     3001 GATCAAGAAC CGTTACACCA AAAGCTGTAA TTTTGAGTTC AACGATGACC AGTTCGGTGA
     3061 TCTGGAAACC ATGCTGAGCG AAGTCCATGA AAAAGGCTTT AAGATGTGCT TTTGGATTAA
     3121 CCCTTATCTG CAAATTGACA ATAGCGAAAT GTGGAACGAA GTCTATGAAA AAGGTTACCA
     3181 TGTTAAGAAA GCAGACGGTT CGATCGCACA CATTTGGTCC GGTTTTGGTA ACTATCAAGA
     3241 AACCGCATCC GCCATTGATC TGACCAATCC GGACGCAGTT GTGTATTGGA AGAATAAGAT
     3301 TAAGGATCTG ATGTACAAAG GCGTCGACTT TATTAAGACC GATTATGGCG ACTCCGTGGA
     3361 TGAGGACGCT GTTATGTTTA ACGGCGAGAA GGGTACGAAT TTTAAGAATG CATACGCGGA
     3421 GCTGTATCTG CGTTACGTGT ATGAGGCGAC CCAGGAGGTG AAGGGCATCG ACAAAGGTTT
     3481 TTGTCTGAGC CGCCCGGGTT ACATCGGCAC CCAGAAATAT GTGGGTAAAT GGGCGGGCGA
     3541 CAGCGCGTCT AGCTTCAATG AGCTGAAGAT GCAACTGCTG AGCGGTCTGT CGAACAGCCT
     3601 GTGCGGTACG GTTATGTGGG GTACGGATAT TGGTGGCTTC TTGGACATCA ATGCGAACGA
     3661 GGAGGACCTG TATGCACGTT GGAGCCAATT CGGTTTGCTG ACGCCGTTCA GCCGTTATCA
     3721 CGGTGTCGGC GCGCGTGAGC CATGGTACTT TGGTGAGAAG GACCTGAATA TCAGCCGCGA
     3781 AGCGGCCAAG CTGAAGCGTC AACTGCTGCC GTACTACAAA ATTTATGAGA AAGAGGCCAT
     3841 CGAAAGCGGT CTGCCGATCA TCCGCCCGCT GGTTCTGGAG TTTCCGAACG ACACCATTGC
     3901 CGCCAAAATT GACGACCAGT TTATGCTGGG CGAGAACATT ATGGTCGCGC CGATTTTGTC
     3961 TAACAAAAAG TATGAGCGTC AGGTCTACTT TCCGGAAGGT AATTGGATCG ATTTCAGCGA
     4021 CAAGAAGATC TATGAGGGTA ACAAGAAATA CAACATTGAC TGCCCGATCG AGAAAATCCT
     4081 GATCTTCGTC AAAGAGAACA GCATTATTCC GATGATTAAG AACCCGATGT ATAAATTTAA
     4141 CAATATCGAC AACGAAAAAA TTAAACTGCG TATCTATGGC AATGTCCTCA ATGAGACTCT
     4201 TAAATTTCGT ATTAACGATA AAGACTATTC TGTTGAAGTG CGTGACAATA AGATTGTCAA
     4261 CGAAAATGAG AAATTTGAGG TTGAGTGAAG AGCTTAGAGA CCCGCATGTA GGCACCATCT
     4321 AT
//