LOCUS       Exported     4295 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4295
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4261
                     /note=""
                     /translation="MNELLTFRGYVVKYGFSNDAGWGTGTFASEDNLKQHMSIKGSITSMDKKTLYEITGYFEEHPRYGKSFNVQTYRKAPIHSTKEQIKFLAGPAFPGVGEKTAEIIINHLGDKAITQLVKNIELLNEIPGLNAIYIPIIKKGIEENIRQEGQERLRYIFFENNLKTSILDWMDNHFDQDNDIIESIFSNSFLSFAKDKEIASFEELDKVAVHFGLELHSPERIAYWAWKLADDFLFASGDTYTNKDYLSRSLMRKLKIQDTELLFEGILYAKEKGILKLTKNRIYTEESWREEQIIADNIIKLNIPTKNANKEGISWEINQIEEEIAYENKIKDFKFDEKQREALELFANNKLSIITGGPGTGKTTLIKGIVKLFNRMSGTEDYAIATPTGRAAARIRETYKKSYATTIHKLLEAKELNKFQITQNNPLNQKLVILDECSMIDNKLFASFIQSCDRVRKVVLVGDANQLPSVGYGNTFADLLELDFLKTIKLDTVHRQKNGNGIVDLAYKVLNETLEISDLESMSNVEINFDYNNQNTMNKVTEIVGQHWDDLEKRANHLQVICPMYGGELGIDEINAQIQNEFNENVKDKKKVYDRGDYRFVVEDKIMFLKNDTDLDLANGDVGKIIKINYDAIGRLKDVLTEFNDKEILLQPKNFSDVKLSYATSVHKTQGSEYNNVLLVIDSPKFNSGFINKTLIYTAVTRAKDKIVILGDSDLFFNAIKITPPRRNTTLIETMLEKRETNG"
                     /label="Mfl322"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAACGATGAT GCTCACTCTC GGGTCTCAAA TGAACGAACT
     2041 GCTGACGTTC CGTGGCTATG TCGTTAAGTA TGGCTTCAGC AACGATGCGG GCTGGGGCAC
     2101 TGGTACCTTC GCTAGCGAGG ACAACCTGAA ACAACATATG AGCATTAAGG GCTCCATCAC
     2161 TTCTATGGAC AAAAAGACCC TGTACGAAAT TACCGGCTAC TTCGAGGAGC ATCCGCGTTA
     2221 TGGTAAGTCC TTTAACGTTC AGACCTACCG TAAGGCCCCG ATTCACTCTA CGAAAGAACA
     2281 GATCAAGTTT CTGGCGGGTC CGGCGTTCCC AGGTGTTGGT GAAAAAACCG CAGAGATCAT
     2341 TATCAACCAC CTGGGCGACA AAGCAATCAC CCAGCTGGTG AAAAACATCG AGCTGTTAAA
     2401 TGAAATTCCG GGTCTGAATG CGATCTACAT TCCGATTATT AAGAAAGGTA TTGAGGAAAA
     2461 TATCCGCCAG GAAGGCCAAG AACGTCTGCG CTACATTTTC TTCGAGAACA ACCTGAAAAC
     2521 GTCCATTCTG GATTGGATGG ACAACCACTT TGACCAAGAC AATGACATTA TCGAAAGCAT
     2581 TTTCAGCAAT AGCTTCCTGA GCTTCGCGAA AGACAAGGAG ATTGCATCGT TCGAAGAACT
     2641 GGACAAGGTT GCAGTGCACT TCGGTTTGGA GCTGCATAGC CCGGAACGTA TCGCATATTG
     2701 GGCTTGGAAG CTGGCAGATG ATTTCCTGTT TGCTAGCGGT GATACGTACA CCAATAAGGA
     2761 CTATCTGAGC CGCAGCCTGA TGCGCAAACT GAAAATCCAA GATACCGAGC TGCTGTTCGA
     2821 AGGTATCCTG TACGCCAAAG AGAAAGGTAT CCTGAAACTG ACGAAAAATC GTATCTACAC
     2881 CGAGGAAAGC TGGCGCGAGG AGCAAATTAT CGCCGATAAT ATTATTAAGC TGAACATTCC
     2941 GACCAAAAAC GCAAATAAAG AAGGTATCAG CTGGGAGATC AACCAGATTG AAGAGGAGAT
     3001 CGCGTACGAA AACAAAATTA AGGACTTCAA GTTTGACGAA AAACAACGTG AGGCGCTGGA
     3061 ACTGTTTGCA AACAATAAAC TGAGCATCAT CACGGGTGGC CCTGGTACCG GCAAAACTAC
     3121 CCTGATCAAA GGTATCGTTA AGCTGTTTAA TCGCATGTCG GGTACGGAGG ATTACGCCAT
     3181 TGCGACCCCA ACCGGCCGTG CGGCCGCCCG TATTCGCGAA ACTTACAAAA AGAGCTATGC
     3241 CACCACGATT CATAAATTGC TGGAGGCAAA AGAGTTGAAT AAATTTCAGA TTACCCAGAA
     3301 CAACCCGTTA AACCAGAAAC TGGTGATTCT TGACGAATGC TCGATGATCG ACAATAAACT
     3361 GTTCGCTAGC TTCATCCAGA GCTGCGACCG CGTGCGCAAA GTTGTGCTGG TCGGCGACGC
     3421 GAACCAGTTG CCGTCTGTTG GCTATGGTAA TACCTTCGCG GATCTGCTGG AATTGGATTT
     3481 CTTAAAGACG ATCAAACTGG ACACGGTTCA CCGTCAGAAG AATGGCAACG GTATCGTCGA
     3541 CCTGGCGTAC AAAGTCCTGA ATGAAACCCT GGAGATTAGC GATCTGGAAA GCATGTCCAA
     3601 CGTGGAAATT AACTTCGACT ACAACAATCA AAATACGATG AATAAGGTTA CCGAAATTGT
     3661 GGGTCAACAC TGGGACGACC TGGAAAAGCG TGCGAACCAT CTGCAAGTTA TCTGCCCAAT
     3721 GTACGGCGGC GAGCTGGGCA TTGACGAAAT TAACGCACAG ATCCAGAATG AGTTCAACGA
     3781 AAATGTCAAA GACAAGAAGA AGGTCTACGA TCGCGGTGAT TATCGCTTTG TCGTTGAAGA
     3841 TAAAATCATG TTCTTGAAGA ACGACACGGA CCTGGACTTG GCAAACGGCG ACGTTGGTAA
     3901 AATCATTAAG ATTAACTACG ATGCAATTGG TCGCCTGAAG GATGTTCTGA CGGAATTTAA
     3961 TGACAAAGAG ATTCTGCTGC AGCCGAAAAA CTTTTCGGAT GTCAAACTGA GCTACGCCAC
     4021 CAGCGTGCAC AAGACCCAGG GTTCCGAGTA CAACAACGTG CTGCTGGTGA TTGATAGCCC
     4081 AAAATTCAAC AGCGGCTTCA TCAATAAGAC CCTGATTTAT ACGGCTGTGA CGCGTGCGAA
     4141 AGACAAAATT GTCATTCTGG GTGATAGCGA TCTGTTTTTC AATGCAATTA AAATTACGCC
     4201 ACCGCGCCGC AACACCACCC TGATCGAAAC GATGCTGGAG AAGCGTGAAA CCAATGGCTG
     4261 AAGAGCTTAG AGACCGAGGT CAATCATGCC TCTCT
//